Study on the Differences in Growth and Metabolomics of Flavonoids in Tissue Culture Seedlings of Lilium pumilum DC. under Different Light Quality

doi:10.15933/j.cnki.1004-3268.2025.07.012

Abstract

Abstract: Under the different light qualities，the differences in growth and flavonoid metabolites of L.pumilum DC. tissue cultured seedlings were analyzed.During the proliferation and growth stage of L.pumilum DC.tissue cultured seedlings，five light quality treatments were set up：white light（W），pure red light（R），pure blue light（B），red blue 1∶1 composite light（R1B1），and red blue green 1∶1∶1 composite light（RGB）. We observed and compared the growth differences of each treatment，and detected flavonoids using UHPLC-MS/MS technology. Under W treatment，the proliferation coefficient of L.pumilum DC.was the highest，at 3.31，the overall growth was better，the leaves were more robust，and the cluster seedlings were more vigorous.Compared with white light quality，red light quality contained 5 differential flavonoids（R_W），2 differential flavonoids were screened out in the B_W，29 differential flavonoids were screened out in the R1B1_W，and 22 differential flavonoids were screened out in the RGB_W.The relative content of differential flavonoids was the highest under RGB treatment，and flavonoids with a higher proportion such as kaempferol and rutin were significantly upregulated. KEGG pathway analysis showed that these substances were mainly enriched in flavonoid and flavonol biosynthesis pathways and flavonoid biosynthesis pathways. What was more important，R1B1 indirectly inhibited the accumulation of kaempferol and downstream substances by regulating the precursor substance naringenin chalcone，while RGB directly positively regulated the accumulation of naringin and downstream flavonoids such as kaempferol.

Key words: Lilium pumilum DC., Light quality, Growth, Metabolomics, Flavonoids

摘要： 为探究不同光质影响下，细叶百合组培苗长势及黄酮类代谢物差异，在细叶百合组培苗增殖生长阶段设置白光（W）、纯红光（R）、纯蓝光（B）、红蓝1∶1复合光（R1B1）和红蓝绿1∶1∶1复合光（RGB）5种光质处理，观察比较长势差异，并利用超高效液相色谱-质谱（UHPLC-MS/MS）联用技术检测黄酮类物质。结果表明，较其他处理，W处理下细叶百合组培苗增殖系数最大，为3.31，且综合长势更好，叶片更加健壮，丛苗更旺盛。红色光质与白色光质相比（R_W，下同），有5种黄酮类差异物质，B_W中有2种黄酮类差异物质，R1B1_W中有29种黄酮类差异物质，RGB_W中有22种黄酮类差异物质；RGB处理下，黄酮类差异物质相对含量较高，且山奈酚、芦丁等占比较大的黄酮物质显著上调。通过KEGG注释，黄酮类差异物质主要富集到黄酮和黄酮醇生物合成途径、类黄酮类生物合成途径，且R1B1处理通过调控山奈酚前体物质柚皮素查尔酮来间接抑制山奈酚及下游物质的积累，RGB处理则直接正向调控柚皮素及下游山奈酚等黄酮类物质的积累。

关键词: 细叶百合, 光质, 长势, 代谢组学, 黄酮类物质

CLC Number:

S682.2⁺65

DENG Yu, HOU Jia, MA Zhengzheng, LI Zhengxiao, LU Songhui. Study on the Differences in Growth and Metabolomics of Flavonoids in Tissue Culture Seedlings of Lilium pumilum DC. under Different Light Quality[J]. Journal of Henan Agricultural Sciences, 2025, 54(7): 116-125.

邓煜, 侯佳, 马正政, 李正潇, 鲁松辉. 不同光质下细叶百合组培苗长势差异及黄酮类物质代谢组学研究[J]. 河南农业科学, 2025, 54(7): 116-125.

Figures/Tables 7

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