Analysis of Aroma Substances of Alcohols，Esters and Ketones in Aerosol of Heated Cigarette Based on Qualitative and Quantitative Techniques

doi:10.15933/j.cnki.1004-3268.2025.06.018

Abstract

Abstract: To provide a reference for the optimization of heating cigarettes，the design of cigarette cartridge and the development of new products，the heating cigarette aerosol was taken as the research object，the qualitative analysis was carried out by gas chromatography‐mass spectrometry（GC‐MS），and the accurate quantitative detection was realized by gas chromatography‐hydrogen flame ionization（GC‐FID）.The simultaneous determination method of 23 kinds of alcohols，esters and ketones in heating cigarette aerosol was established.By comparing the extraction effects of different ultrasonic extraction parameters（ultrasonic time，extraction solvent dosage，ultrasonic power）on the 23 aromatic substances，the optimal extraction method was determined；meanwhile，the established ultrasonic extraction gas chromatography hydrogen flame ionization（UE‐GC‐FID）method was used to detect the content changes of 23 aromatic substances in the mainstream smoke of heated cigarettes under different heating methods（resistance heating and infrared heating），different flavors［original flavor（OR）and menthol flavor（ME）］and different temperatures（150.00，200.00，250.00，300.00℃）.The results showed that the optimum extraction conditions were as follows：ultrasonic time was 30.00 min，extraction solvent dosage was 25.00 mL，ultrasonic power was 500.00 W.The linearity of the method was good in the range of 10.00—240.00 μg/mL（R²≥0. 991 4），and the limit of detection（LOD）and limit of quantitation（LOQ）of 23 aromatic substances were 0.075 5—1.285 8 μg/mL and 0.241 0—4.148 0 μg/mL，respectively.The intra‐day RSD and inter‐day RSD were 0.241 8%—2.128 7% and 1.556 6%—4.812 5%，respectively，and the average spiked recoveries of the three gradients were 94.380 3%—104.645 0%.The content of alcohols was the highest under the conditions of resistance heating，ME taste and 300.00 ℃，which was 16.318 0 μg/cig.The highest content of esters was 12.556 5 μg/cig under the conditions of infrared heating，OR taste and 300.00 ℃.The content of ketones was the highest under the conditions of infrared heating，OR taste and 300.00 ℃，which was 22.712 0 μg/cig.The total content of 23 aromatic substances was the highest under the conditions of infrared heating，OR taste and 300.00 ℃，which was 50.347 6 μg/cig.However，there were significant differences in the optimal release conditions of different components.In conclusion，the method is simple，rapid and can fully meet the needs of accurate quantitative analysis of 23 kinds of alcohols，esters and ketones in heated cigarette smoke.

Key words: Heating cigarette, Aroma substance, Detection method, Heating method, Temperature, Taste

摘要： 为了给加热卷烟烟具优化、烟弹设计及新产品开发提供参考依据，以加热卷烟气溶胶为研究对象，采用气相色谱-质谱（GC-MS）进行定性分析，结合气相色谱-氢火焰离子化法（GC-FID）实现准确定量检测，建立加热卷烟气溶胶中23种醇类、酯类、酮类香味物质的同时测定方法。通过对比不同超声提取参数（超声时间、萃取溶剂用量、超声功率）对23种香味物质的提取效果，确定最佳提取方案；同时利用建立的超声萃取气相色谱氢火焰离子化（UE-GC-FID）的方法，检测不同加热方式（电阻加热、红外加热）、不同口味［原味（OR）、薄荷味（ME）］、不同温度（150.00、200.00、250.00、300.00℃）条件下加热卷烟主流烟气中23种香味物质的含量变化。结果表明，样品最佳提取条件为超声时间30.00 min、萃取溶剂用量25.00 mL、超声功率500.00 W；该方法在10.00~240.00 μg/mL范围内线性良好（R²≥0.991 4），23种香味物质检出限（LOD）及定量限（LOQ）分别为0.075 5~1.285 8 μg/mL、0.241 0~4.148 0 μg/mL；日内精密度和日间精密度分别为0.241 8%~2.128 7%、1.556 6%~4.812 5%；3个梯度平均加标回收率为94.380 3%~104.645 0%。醇类物质在电阻加热、ME口味、300.00 ℃条件下含量最高，为16.318 0 μg/支；酯类物质在红外加热、OR 口味、300.00℃条件下含量最高，为12.556 5 μg/支；酮类物质在红外加热、OR 口味、300.00℃条件下含量最高，为22.712 0 μg/支；23种香味物质总量则在红外加热、OR口味、300.00℃条件下最高，为50.347 6 μg/支；但不同成分最佳释放条件存在显著差异。综上，该方法操作简单、快速且完全可以满足加热卷烟烟气中23种醇类、酯类、酮类香味物质准确定量分析的需要。

关键词: 加热卷烟, 香味物质, 检测方法, 加热方式, 温度, 口味

CLC Number:

S572

XUE Yun, LIU Qibin, WU Yan, LI Jingxin, HU Ziyun, LU Li, LI Dian, YAN Jun, HUANG Zhonghui, JING Yanqiu. Analysis of Aroma Substances of Alcohols，Esters and Ketones in Aerosol of Heated Cigarette Based on Qualitative and Quantitative Techniques[J]. Journal of Henan Agricultural Sciences, 2025, 54(6): 163-174.

薛云, 刘启斌, 吴彦, 李京鑫, 胡紫雲, 陆漓, 李典, 严俊, 黄忠辉, 景延秋. 基于定性定量联用技术的加热卷烟气溶胶中醇类、酯类、酮类香味物质分析[J]. 河南农业科学, 2025, 54(6): 163-174.

Figures/Tables 7

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[3]	LI Wan, CHENG Ruixing, DANG Xin. Identification and Expression Analysis of HD‑ZIP Family in Salvia miltiorrhiza [J]. Journal of Henan Agricultural Sciences, 2024, 53(7): 66-78.
[4]	SONG Zonghao, JIANG Chenguang, WU Yuexuan, WU Zhongxin, WANG Longfei, LU Xiaochong, TI Jinsong, LIU Jianjun. Analysis of Chemical Composition Change and Coupling with Temperature and Humidity in Box Cured Tobacco Leaves [J]. Journal of Henan Agricultural Sciences, 2024, 53(6): 162-172.
[5]	YE Zhihui, SHAO Xinguang, ZHAO Xiaozhong. Effects of Different Growth‑Promoting Fungal Fertilizers on Aroma Components，Sensory Quality and Soil Properties of Flue‑cured Tobacco [J]. Journal of Henan Agricultural Sciences, 2024, 53(6): 37-48.
[6]	QI Xianke, LI Miao, LI Caihong, QU Chenling. Effects of PAW and Its Combined Action with LTP on Seed Germination and Seedling Growth of Wheat [J]. Journal of Henan Agricultural Sciences, 2024, 53(4): 20-29.
[7]	ZHAO Xin, CHEN Dong, GUO Guanting, WU Jiaojiao, ZHANG Yuandong, LONG Linmei, LONG Mingzhong, LI Xiaona. Effects of Biocrusts on Soil Nitrogen Mineralization in the Rocky Desertification Area of Guizhou [J]. Journal of Henan Agricultural Sciences, 2024, 53(2): 75-84.
[8]	FAN Yifan, ZHANG Yanyan, WANG Yimei, LI Junzhou, DU Yanxiu, SUN Hongzheng, PENG Ting, ZHAO Quanzhi, ZHANG Jing. Effects of Temperature and Light Conditions on Yield and Quality of Indica Rice under Different Sowing Dates [J]. Journal of Henan Agricultural Sciences, 2024, 53(2): 17-27.
[9]	LI Chuan, ZHANG Panpan, ZHANG Meiwei, NIU Jun, MU Weilin, GUO Hanxiao, QIAO Jiangfang. Screening of miRNAs Related to High Temperature Stress in Maize Pollen and Analysis of Their Target Genes [J]. Journal of Henan Agricultural Sciences, 2024, 53(2): 1-16.
[10]	WEI Xin, WANG Sheng, WANG Xingdong, YANG Yuchun, LIU Youchun, LIU Cheng. Physiological Response and Cold Resistance Evaluation of Blueberry Varieties under Low Temperature Treatment [J]. Journal of Henan Agricultural Sciences, 2023, 52(8): 115-125.
[11]	CHEN Jiading, HE Rong, XIAO Qingli, YUAN Ming, TAN Qizhong, PENG Kui, WEI Shuo, LI Shengchun. Influence of Temperature and Moisture Content on Thermophysical Properties of Tobacco Leaves and Establishment of Prediction Models [J]. Journal of Henan Agricultural Sciences, 2023, 52(6): 172-180.
[12]	LEI Mengyao, GAO Xiaofeng, BAI Qingmin, DENG Ke, ZUO Weifang, LI Yuying. Evaluation of Cold Resistance of Pomegranate Branches from Different Varieties [J]. Journal of Henan Agricultural Sciences, 2023, 52(6): 120-130.
[13]	HUANG Jiaqing, LIU Cenwei, YE Jing, WANG Yixiang, REN Lihua. Physicochemical Properties and Microstructure Changes of Tomato Straw Hydrothermal Biochar under Different Reaction Conditions [J]. Journal of Henan Agricultural Sciences, 2023, 52(5): 170-180.
[14]	WANG Yakun, LI Hongping, XU Zhenzhen, LIU Tianxue. Effects of Combined Stress of High Temperature and Drought on Photosynthetic Physiology of Maize [J]. Journal of Henan Agricultural Sciences, 2023, 52(5): 17-23.
[15]	WANG Ran, LI Yuke, YI Yin, TANG Ming. Research Progress on Chemical Structure，Toxic Effects and Detection Methods of Alternaria Toxins [J]. Journal of Henan Agricultural Sciences, 2023, 52(3): 1-11.