Differences in Physiological Responses of Different Drought⁃tolerant Turnip Seedlings to Drought Stress and Rehydration

doi:10.15933/j.cnki.1004-3268.2025.02.013

Abstract

Abstract: In order to clarify the effects of drought stress and rehydration on the physiological characteristics of turnip seedlings ［Huangqiamagu（B1）：drought⁃resistant type；Qiamagu（B2）：drought⁃sensitive type）］，pot culture was used to control water. The relative conductivity，malondialdehyde（MDA），osmotic adjustment substances［soluble sugar（SS），soluble protein（SP），proline（Pro），reducing sugar］，antioxidant enzymes［superoxide dismutase（SOD），peroxidase（POD），catalase（CAT）］，functional substances（total phenols，flavonoids，anthocyanins）and endogenous hormones［auxin（IAA），abscisic acid（ABA），trans⁃zeatin nucleoside（ZR），gibberellin（GA₃）］ of leaves after mild drought stress and severe drought stress and rehydration were studied.The results showed that with the increase of drought degree，the relative conductivity and MDA content of leaves of two turnip seedlings increased gradually，while the increase of B1 was smaller. Under drought stress，the contents of Pro and SS in the leaves of B1 and B2 germplasm seedlings increased gradually，the content of reducing sugar increased first and then decreased，the content of SP in B1 germplasm increased first and then decreased，and B2 increased gradually. The activities of SOD and POD in leaves of B1 and B2 germplasm seedlings were in direct proportion to the degree of drought stress，with the maximum value of CAT in B1 under severe drought stress and the maximum value of CAT in B2 under mild drought stress.The contents of total phenols and flavonoids in leaves of B1 and B2 germplasm seedlings increased gradually.The anthocyanin content of B1 increased gradually，while B2 increased first and then decreased.And the change range of B2 indexes in each treatment was larger than that of B1. With the increase of drought degree，IAA，ABA and ZR increased first and then decreased，while GA₃ decreased gradually，and the contents of IAA，ZR and GA₃ in B1 were lower than those in B2.After rehydration，the contents of all physiological indexes recovered except POD of B2.To sum up，drought and rehydration have different effects on the physiological characteristics of leaves of different drought⁃resistant turnip germplasms.Under drought stress，drought⁃resistant germplasm is less affected and shows stronger drought adaptability and resilience.

Key words: Turnip seedlings, Drought stress, Rehydration, Physiological characteristics

摘要： 为明确干旱胁迫和复水对芜菁幼苗生理特性的影响，以芜菁幼苗［黄恰玛古（B1），抗旱型；恰玛古（B2），干旱敏感型］为研究对象，采用盆栽控水法，研究叶片在轻度干旱胁迫、重度干旱胁迫以及复水后的相对电导率、丙二醛（MDA）含量、渗透调节物质［可溶性糖、可溶性蛋白、脯氨酸、还原糖］含量、抗氧化物酶［超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）］活性、功能物质（总酚、类黄酮、花青素）含量及内源激素［生长素（IAA）、脱落酸（ABA）、反式玉米素核苷（ZR）、赤霉素（GA₃）］含量的变化。结果表明，随着干旱程度增加，2种芜菁幼苗叶片的相对电导率和MDA含量逐渐增加，B1增幅较小。干旱胁迫下，B1、B2种质幼苗叶片的脯氨酸、可溶性糖含量呈逐渐增加的趋势，还原糖含量先增加后降低，B1种质可溶性蛋白含量呈先增加后降低的趋势，B2呈逐渐增加的趋势；B1、B2种质幼苗叶片的SOD、POD活性与干旱胁迫程度呈正比，B1在重度干旱胁迫时CAT活性有最大值，B2在轻度干旱胁迫时有最大值；B1、B2种质幼苗叶片总酚、类黄酮含量逐渐增加，B1花青素含量逐渐增加，而B2先增加后降低；且各处理时期中B2种质指标变化幅度较B1大。随干旱程度增加，IAA、ABA、ZR含量呈先上升后下降的趋势，GA₃含量呈逐渐降低的趋势，且B1的IAA、ZR、GA3含量较B2低。复水后，除B2的POD活性外，各生理指标含量恢复。综上，干旱以及复水对不同抗旱型芜菁种质叶片生理特性的影响不同，干旱胁迫下，抗旱型种质受到的影响小且表现出更强的干旱适应能力和恢复能力。

关键词: 芜菁幼苗, 干旱胁迫, 复水, 生理特性

CLC Number:

S631.3

HE Miao, JIANG Yuanhao, MIN Haozhe, LIN Tao, WANG Duo, XUAN Zhengying. Differences in Physiological Responses of Different Drought⁃tolerant Turnip Seedlings to Drought Stress and Rehydration[J]. Journal of Henan Agricultural Sciences, 2025, 54(2): 116-123.

何苗, 姜元昊, 闵昊哲, 林涛, 王铎, 轩正英. 不同抗旱型芜菁幼苗对干旱胁迫及复水的生理响应差异[J]. 河南农业科学, 2025, 54(2): 116-123.

Figures/Tables 6

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