Abstract: Morganella morganii is an opportunistic pathogen that can cause a variety of diseases and has become an important emerging pathogen worldwide.In order to explore the drug sensitivity and pathogenicity of Morganella morganii from swine,a suspected pathogenic strain was isolated from the intestines of dead pigs in Henan Province.Gram staining,matrix‑assisted laser desorption/ionization time of flight mass spectrometry and 16S rRNA gene homology analysis were performed.Then the minimum inhibitory concentration of 14 drugs against the strain was determined,and the infection experiment wa conducted in mice.The results showed that the bacterium was Gram‑negative brevibacterium，which was identified as Morganella morgenii and named A185.The results of drug susceptibility test showed that the strain was resistant to amoxicillin,meropenem,igacycline,polymyxin,doxycycline and cefoxitin,intermediate to ciprofloxacin and gentamicin,and sensitive to fosfomycin and ceftazidime.The results of infection experiment showed that the Kunming mice inoculated with Morganella morganii exhibited hemorrhage in liver,intestine and lung to varying degrees.The above results showed that the porcine Morganella morganii A185 had multi‑drug resistance,and exhibited strong pathogenicity.

Key words: Morganella morganii, Isolation and identification, Drug sensitive test, 16S rRNA, Pathogenicity

摘要： 摩氏摩根菌是一种可导致多种疾病的条件性病原菌，已成为全球范围内的一种重要新发病原菌。为了解猪源摩氏摩根菌的药物敏感性和致病性，从河南省某猪场病死猪肠道中分离得到一株疑似致病菌，对其进行革兰氏染色、基质辅助激光解吸/电离飞行时间质谱鉴定和16S rRNA基因同源性分析，然后测定14种药物对该菌株的最小抑菌浓度，并进行小鼠感染试验。结果显示，该菌为革兰氏阴性短杆菌，鉴定为摩氏摩根菌，命名为A185。药敏试验结果表明，该菌对阿莫西林、美罗培南、替加环素、多黏菌素、多西环素、头孢西丁耐药；对环丙沙星、庆大霉素中度敏感；对磷霉素、头孢他啶敏感。小鼠感染试验结果显示，感染摩氏摩根菌的昆明鼠表现为肝脏、肠道和肺脏不同程度出血。以上结果表明，猪源摩氏摩根菌A185具有多重耐药性和较强的致病性。

关键词: 猪源摩氏摩根菌, 分离鉴定, 药敏试验, 16S rRNA, 致病性