Effect of Fermented Traditional Chinese Medicine on Intestinal Microflora of Broilers with Avian Colibacillosis

doi:10.15933/j.cnki.1004-3268.2022.07.015

Abstract

Abstract: This study aimed to evaluate the effect of fermented traditional Chinese medicine on intestinal microflora of broilers with avian colibacillosis.270 healthy 1‑day‑old AA white‑feathered broilers were divided into three groups，three replicates for each treatment and 30 broilers for each repetition.Broilers in the control group（D group）were routinely fed.The fermented traditional Chinese medicine group （PEG group）and the antibiotic group（K group）were modeled with E.coli O78 and treated.The samples were analyzed through 16S rRNA sequencing technology.The result showed that after treatment with the fermented traditional Chinese medicine composed of Andrographis herba，Pulsatillae Radix，Patrinia，Rhizoma Coptidis，etc，there was no significant difference in the richness and diversity of microflora with the control group，and the content of Akkermansia was significantly increased.The fermented traditional Chinese medicine can regulate intestinal microflora and substance transport and metabolism，increase the abundance of beneficial bacteria and inhibit harmful bacteria in broilers.

Key words: Fermented traditional Chinese medicine, Avian colibacillosis, Intestinal microflora, 16S rRNA, Broiler

摘要： 为研究发酵中药对感染禽大肠杆菌病肉仔鸡肠道微生物区系的影响，选择270只1日龄爱拔益加（AA）白羽肉鸡，随机分成3组，每组3个重复，每个重复30只。对照组（D组）常规饲养，发酵中药组（PEG组）和抗生素组（K组）分别用致病性大肠杆菌O78造模并治疗。采用16S rRNA测序技术对微生物菌群特征进行分析。结果显示，由穿心莲、白头翁、败酱草、黄连等中药制备的发酵中药，治愈禽大肠杆菌病后的肠道微生物区系在菌群丰富度和多样性上与对照组无显著差异，且能够显著提高Akkermansia在肠道菌群中的比例。综上，发酵中药可调节肉鸡肠道微生物群落结构，提高有益菌群丰度，降低有害菌群丰度，调节物质运输和代谢，进而改善肠道菌群结构。

关键词: 发酵中药, 禽大肠杆菌病, 肠道菌群, 16S rRNA, 肉鸡

CLC Number:

S859.3

GU Yanli, CAO Yanzi, WANG Xiaoyu, SHAN Chunqiao, LIU Yan, JIANG Guotuo. Effect of Fermented Traditional Chinese Medicine on Intestinal Microflora of Broilers with Avian Colibacillosis[J]. Journal of Henan Agricultural Sciences, 2022, 51(7): 145-153.

顾艳丽, 曹艳子, 王效禹, 单春乔, 刘艳, 江国托. 发酵中药对禽大肠杆菌病肉鸡肠道菌群的影响[J]. 河南农业科学, 2022, 51(7): 145-153.

References

［1］顾艳丽，刘秋晨，林洋，等.复方中药发酵液对肉鸡生长性能、肠道结构及免疫功能的影响［J］.中国畜牧杂志，2021，57（4）：211‑215.
GU Y L，LIU Q C，LIN Y，et al.Effects of compound Chinese medicine fermentation broth on growth performance intestinal structure and immune function of broilers［J］.Chinese Journal of Animal Science，2021，57（4）：211‑215.
［2］顾艳丽，单春乔，曹艳子，等.复方中药发酵液对肉鸡大肠杆菌病的防治效果及免疫调节作用［J］.中国畜牧兽医，2020，47（11）：3687‑3695.
GU Y L，CHAN C Q，CHAO Y Z，et al.Effect of fermented traditional Chinese medicine on preventing and treating colibacillosis and immune regulation in broilers［J］.China Animal Husbandry & Veterinary Medicine，2020，47（11）：3687‑3695.
［3］GEVA‑ZATORSKY N，SEFIK E，KUA L，et al.Mining the human gut microbiota for immunomodulatory organisms［J］.Cell，2017，168（5）：928‑943.
［4］GLENWRIGHT A J，POTHULA K R，BHAMIDMARRI S P，et al.Structural basis for nutrient acquisition by dominant members of the human gut microbiota［J］.Nature，2017，541：407‑411.
［5］BARKO P C，MCMICHAEL M A，SWANSON K S，et al.The gastrointestinal microbiome：A review［J］.Journal of Veterinary Internal Medicine，2018，32（1）：9‑25.
［6］THAISS C A，ZMORA N，LEVY M，et al.The microbiome and innate immunity［J］.Nature，2016，535：65‑74.
［7］李智杰，赵琦，吴华星.通过宏基因组学研究肠道微生物对结直肠癌患者影响的最新进展［J］.实用肿瘤学杂志，2020，34（1）：93‑96.
LI Z J，ZHAO Q，WU H X.Research progress in the effects of intestinal microbes on colorectal cancer patients by metagenomics［J］.Practical Oncology Journal，2020，34（1）：93‑96.
［8］许腾林，邢桂玲，姜成刚，等.猪、鸡与貂源肠外致病性大肠杆菌群型、耐药性与致病性分析［J］.中国预防兽医学报，2018，40（5）：386‑391.
XU T L，XING G L，JIANG C G，et al.Phylogenetic group，antimicrobial resistance and pathogenic analysis of
extraintestinal pathogenic Escherichia coli isolated from swine，chicken and mink［J］.Chinese Journal of Preventive Veterinary Medicine，2018，40（5）：386‑391.
［9］郭晓宇，韩俊英，闫素梅，等.维生素A对肉鸡生长性能、免疫及抗氧化功能的影响［J］.动物营养学报，2019，31（8）：3582‑3589.
GUO X Y，HAN J Y，YAN S M，et al.Effects of vitamin A on growth performance immunity and antioxidant function
of broilers［J］.Chinese Journal of Animal Nutrition，2019，31（8）：3582‑3589.
［10］朱见深，王进文，张庆，等.SPF鸡粪菌移植对雏鸡肠道菌群和大肠杆菌抗药性的影响［J］.山东农业科学，2018，50（7）：6‑12.
ZHU J S，WANG J W，ZHANG Q，et al.Effect of fecal microbiota transplantation from SPF chickens on intestinal flora and E. coli resistance to antibiotics in chicks［J］.Shandong Agricultural Sciences，2018，50（7）：6‑12.
［11］张红娜，周玉法，崔娜，等.基于高通量测序和Q-PCR芯片技术分析养殖环境中苍蝇携带细菌菌群结构和耐药基因特点［J］.东北农业大学学报，2020，51（11）：70‑79.
ZHAGN H N，ZHOU Y F，CUI N，et al.Study on bacterial community and antibiotic resistance genes of flies in breeding environments by high‑throughput sequencing and Q‑PCR array［J］.Journal of Northeast Agricultural University，2020，51（11）：70‑79.
［12］鹿欣雨，李正堃，李海燕.肠道细菌Akkermansia muciniphila在疾病调控中的作用研究进展［J］.医学研究生学报，2021，34（8）：856‑860.
LU X Y，LI Z K，LI H Y.Advances of Akkermansia muciniphila in disease regulation［J］.Journal of Medical Postgraduates，2021，34（8）：856‑860.
［13］郝贺，钟翠红，李欣泽，等.发酵中药对热应激肉鸡肠道菌群结构的影响［J］.河南农业科学，2021，50（9）：135‑142.
HAO H，ZHONG C H，LI X Z，et al.Effect of fermented traditional Chinese medicine on intestinal flora structure
of broilers under heat stress［J］.Journal of Henan Agricultural Sciences，2021，50（9）：135‑142.
［14］王其龙，杨景森，黄凯勇，等.小檗碱调控动物肠道菌群稳态的研究进展［J］.中国畜牧杂志，2020，58（2）：23‑26，31.
WANG Q L，YANG J S，HUANG K Y，et al.Research progress on berberine regulating homeostasis of gut microbiota in animals［J］.Chinese Journal of Animal Science，2020，58（2）：23‑26，31.
［15］王新红，张迟，刘琳，等.皂苷类成分与肠道菌群相互作用研究进展［J］.中成药，2021，43（7）：1834‑1839.
WANG X H，ZHANG C，LIU L，et al.Research progress on the interaction between saponins and intestinal flora
［J］.Chinese Traditional Patent Medicine，2021，43（7）：1834‑1839.
［16］NICOLAI K，ELEONORA N，MICHAL P，et al.Genomic diversity and ecology of human‑associated Akkermansia species in the gut microbiome revealed by extensive metagenomic assembly［J］.Genome biology，2021，22（1）：209.
［17］LIU C，HU B，CHENG Y，et al.In‑depth analysis of the mechanisms of aloe polysaccharides on mitigating subacute colitis in mice via microbiota informatics［J］.Carbohydrate Polymers，2021，265：118041.
［18］吴艳丽，刘朋，苏咏欣，等.嗜黏蛋白阿克曼氏菌ATCC BAA-835肠道益生作用的体外评价［J］.食品与发酵工业，2022，48（2）：156‑162.
WU Y L，LIU P，SU Y X，et al.Evaluation of the intestinal probiotic effect of Akkermansia muciniphila ATCC BAA‑835 in vitro［J］.Food and Fermentation Industries，2022，48（2）：156‑162.
［19］DONG C R，YU J Q，YANG Y N，et al.Berberine，a potential prebiotic to indirectly promote Akkermansia
growth through stimulating gut mucin secretion［J］.Biomedicine & Pharmacotherapy，2021，139：111595.
［20］杨光勇，涂小华，游丰锋，等.基于qPCR技术分析黄连对溃疡性结肠炎小鼠肠道内嗜黏蛋白阿克曼氏菌的影响［J］.中医药学报，2021，49（11）：18‑23.
YANG G Y，TU X H，YOU F F，et al.Effect of Coptis chinensis on intestinal Akkermansia muciniphila in mice with UC based on qPCR［J］.Acta Chinese Medicine and Pharmacology，2021，49（11）：18‑23.
［21］刘阳.Akkermansia muciniphila菌对肠道屏障的调节作用及相关基因研究［D］.无锡：江南大学，2021：4‑5.
LIU Y.The research on the regulation of Akkermansia muciniphila strains on intestinal barrier and related functional genes［D］.Wuxi：Jiangnan University，2021：4‑5.