Journal of Henan Agricultural Sciences ›› 2022, Vol. 51 ›› Issue (6): 119-125.DOI: 10.15933/j.cnki.1004-3268.2022.06.013

• Horticulture • Previous Articles     Next Articles

Identification and Analysis of the ZF⁃HD Gene Family in Punica granatum

FU Hongbo,LI Jie,YANG Yongchao,YUAN Shengyong   

  1. (Key Laboratory for Research and Utilization of Characteristic Biological Resources in Southern Yunnan,College of Biological and Agricultural Sciences,Honghe University,Mengzi 661199,China)
  • Received:2021-10-18 Published:2022-06-15 Online:2022-08-16

石榴ZF-HD 基因家族鉴定与分析

付鸿博,李杰,杨永超,袁盛勇   

  1. (红河学院生物科学与农学学院,云南省高校滇南特色生物资源研究与利用重点实验室,云南 蒙自 661199)
  • 通讯作者: 李杰(1987-),男,甘肃天水人,副教授,博士,主要从事设施园艺作物栽培与环境调控工作。E-mail:gsau23@126.com
  • 作者简介:付鸿博(1987-),男,吉林通化人,讲师,博士,主要从事果树种质资源创新利用与分子育种工作。E-mail:113311168@qq.com
  • 基金资助:
    云南省中青年学术和技术带头人后备人才项目(2021HB075);红河学院科研基金博硕专项(XJ17B07)

Abstract: The gene family of ZF⁃HD was identified through a genome⁃wide study of Punica granatum to gain a deeper understanding of its function. Based on the systematic bioinformatics analyses,fifteen PgZF-HD genes were identified,the number of encoded amino acids ranged from 98 to 365,PgZF⁃HD03 and PgZF⁃HD04 were acidic proteins,and other family members were basic proteins,all family members were unstable hydrophilic proteins,more than 70% PgZF⁃HD proteins were located in the nuclear.In the secondary structure,the proportion of beta⁃turn was the lowest,and that of random coil was the highest.
Fifteen PgZF‑HD genes were classified into five categories by phylogenetic tree analysis,namely ZF⁃HDⅠ,ZF⁃HD Ⅱ ,ZF⁃HD Ⅲ ,ZF⁃HD Ⅴ and MIF. The ZF⁃HD Ⅲ category contained the most members of PgZF‑HD,no PgZF‑HD gene was classified in the ZF⁃HDⅣ category. It was found that both motif2 and motif4 were highly conserved and contained multiple highly conserved active sites.The largest amount of PgZFmotif was found in ZF⁃HDⅡ,and motif8 was identified only in ZF⁃HDⅠ.Most PgZF‑HD genes had only one exon,the gene structure was simple,and the same class of genes had similar conservative motif and gene structure.Chromosome mapping and gene duplication analysis showed that 15 PgZF‑HD genes were inhomogeneously distributed on eight chromosomes,three gene pairs were identified to have large fragment duplication events and no tandem duplication events occurred.Promoter analysis showed that the PgZF‑HD genes played an important role in plant hormone response,growth and development,and stress response.

Key words: Punica granatum, ZF?HD, Bioinformatics, Promoter analysis, Gene duplication event

摘要: 通过对石榴(Punica granatum)全基因组的研究,鉴定了ZF-HD基因家族,以深入了解其基因功能。基于系统的生物信息学分析,共鉴定15个PgZF-HD 基因,编码蛋白质的氨基酸数量在98~365个,PgZF-HD03和PgZF-HD04为酸性蛋白质,其余的家族成员均为碱性蛋白质,所有家族成员均为不稳定的亲水性蛋白质,超过70%的蛋白质定位在细胞核上。二级结构中,β-折叠所占比例最低,无规则卷曲所占比例最高。系统进化树分析将15个PgZF-HD 基因分成5类,分别为ZF-HDⅠ、ZF-HDⅡ、ZF-HDⅢ、ZF-HDⅤ和MIF,ZF-HDⅢ类别中包含了最多的PgZF-HD 成员,没有PgZF-HD基因被归到ZF-HDⅣ类别中。基因结构和保守基序分析发现,motif2和motif4高度保守且均含有多个高度保守的活性位点,ZF-HDⅡ类别中motif数量最多,motif8只在ZF-HDⅠ类别的基因中被鉴定到。大部分PgZF-HD基因只有1个外显子,基因结构简单,同一类别的基因具有相似的保守基序和基因结构。染色体定位和基因复制分析发现,15个PgZF-HD基因不均匀地分布在石榴的8条染色体上,鉴定到3对基因存在大片段复制事件,不存在串联重复事件。启动子分析表明,PgZF-HD基因在植物激素响应、生长发育和逆境胁迫响应等方面均发挥重要作用。

关键词: 石榴, ZF-HD, 生物信息学, 启动子分析, 基因复制事件

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