Regulatory Mechanism of Carvone on Potato Pre‐elite Seed Sprouting via Reactive Oxygen Species and Energy Metabolism

doi:10.15933/j.cnki.1004-3268.2026.08.016

Abstract

Abstract: To clarify the regulatory effect and mechanism of carvone on the germination of potato seed tubers，two levels of reactive oxygen species and energy metabolism were analyzed with the Atlantic pre‐elite seed as experimental material. The experiment was carried out under dark conditions at room temperature（22±2）℃ and relative humidity of 15%—25%.The pre‐elite seed without treatment were used as the control group（CK），and the treatment with 0.3 mL/kg carvone was used as the experimental group（XQT）.The indicators related to antioxidant and energy metabolism of tuber samples from two groups were determined. Antioxidant enzymes included NADPH oxidase（NOX），catalase（CAT），peroxidase（POD），superoxide dismutase（SOD），glutathione reductase（GR）and ascorbate peroxidase（APX）；the contents of ascorbic acid（AsA），reduced glutathione（GSH） and superoxide anion（O₂^-·）were also measured.Energy metabolism‐related enzymes contained succinate dehydrogenase（SDH），cytochrome C oxidase （CCO），H⁺‐ATPase and Ca²⁺‐ATPase.Meanwhile，the contents of adenosine monophosphate（AMP），adenosine diphosphate（ADP）and adenosine triphosphate（ATP）were determined，and energy charge（EC）was calculated accordingly.The results showed that the regulation of carvone treatment on the sprouting of potato pre‐elite seeds exhibited stage‐specific differences.During the enforced dormancy period of tubers（experimental stage Ⅰ），both XQT group and CK group showed the effect of inhibiting potato sprouting. During the sprouting period of the control tubers（experimental stageⅡ），compared with the CK group，carvone treatment significantly inhibited the activities of NOX，CAT，POD，APX and GR（the declines were 12.12%，51.14%，37.12%，27.40% and 15.33%，respectively），and significantly reduced the contents of AsA，GSH and O₂^-·（the declines were 10.35%，20.15% and 14.63%，respectively）.At the same time，the activities of H⁺‐ATPase，Ca²⁺‐ATPase，SDH and CCO were significantly increased（the increases were 24.60%，60.74%，20.72% and 8.93%，respectively），and the contents of ATP，ADP and AMP were significantly increased（the increases were 7.59%，9.92% and 8.76%，respectively）.At the tuber sprouting stage of XQT（stage Ⅲ），the change trend of each index was opposite to that of stage Ⅱ.Compared with CK group，the activities of NOX，SOD，CAT，POD，APX and GR were significantly increased by 61.04%，13.68%，23.44%，19.92%，14.64% and 7.89%，respectively.The contents of AsA，GSH，O₂^-· and H₂O₂ were significantly increased by 8.38%，28.81%，37.85% and 67.37%，respectively.The activities of H⁺‐ATPase，Ca²⁺‐ATPase，SDH and CCO were significantly decreased by 22.26%，12.87%，14.13% and 9.47%，respectively，and the contents of ATP and ADP were significantly decreased by 8.61% and 13.49%，respectively.In summary，active oxygen species metabolism and energy metabolism in different storage stages interact with each other and jointly regulate the germination of potato pre‐elite seed.

Key words: Potato, Pre‐elite seed, Carvone, Sprouting regulation, Reactive oxygen species metabolism, Energy metabolism

摘要： 为明确香芹酮对马铃薯种薯块茎发芽的调控作用及其机制，着眼于活性氧和能量代谢2个层面展开分析。以大西洋原原种为试验材料，在室温（22±2）℃、相对湿度15%~25%的黑暗条件下进行试验。以不做处理的原原种为对照组（CK），以添加0.3 mL/kg香芹酮处理为试验组（XQT）。分别测定2组原原种抗氧化与能量代谢相关指标：抗氧化系统酶包括NADPH氧化酶（NOX）、过氧化氢酶（CAT）、过氧化物酶（POD）、超氧化物歧化酶（SOD）、谷胱甘肽还原酶（GR）、抗坏血酸过氧化物酶（APX），配套检测抗坏血酸（AsA）、还原型谷胱甘肽（GSH）、超氧阴离子（O₂^·-）含量；能量代谢相关酶为琥珀酸脱氢酶（SDH）、细胞色素C氧化酶（CCO）、H+-三磷酸腺苷酶（H⁺-ATP酶）、Ca2+-三磷酸腺苷酶（Ca²⁺-ATP酶），同时测定一磷酸腺苷（AMP）、二磷酸腺苷（ADP）、三磷酸腺苷（ATP）含量并计算能荷（EC）。结果表明，香芹酮处理对马铃薯原原种发芽的调控呈现阶段性差异。在块茎被迫休眠期（试验阶段Ⅰ），XQT组和CK组均表现出抑制马铃薯发芽的效果。在对照块茎发芽期（试验阶段Ⅱ），与CK组相比，香芹酮处理显著抑制了NOX、CAT、POD、APX和GR活性（降幅分别为12.12%、51.14%、37.12%、27.40%和15.33%），显著降低了AsA、GSH和O₂^- ·含量（降幅分别为10.35%、20.15%和14.63%），同时显著提高H⁺-ATPase、Ca^²⁺-ATPase、SDH和CCO活性（增幅分别为24.60%、60.74%、20.72%和8.93%），显著增加ATP、ADP和AMP含量（增幅分别为7.59%、9.92%和8.76%）。在处理块茎发芽期（试验阶段Ⅲ），各指标变化趋势与阶段Ⅱ相反，NOX、SOD、CAT、POD、APX 和GR 活性分别较CK 组显著增加61.04%、13.68%、23.44%、19.92%、14.64% 和7.89%，AsA、GSH、O₂^- ·和H₂O₂含量分别显著增加8.38%、28.81%、37.85%和67.37%，而H⁺-ATPase、Ca^²⁺-ATPase、SDH和CCO活性分别显著降低22.26%、12.87%、14.13%和9.47%，ATP和ADP含量分别显著降低8.61%和13.49%。综上，不同贮藏阶段活性氧代谢与能量代谢相互影响、共同调控马铃薯原原种的发芽。

关键词: 马铃薯, 原原种, 香芹酮, 发芽调控, 活性氧代谢, 能量代谢

CLC Number:

S532

Li Shouqiang, Ge Xia, Tian Jiachun, Li Mei, Cheng Jianxin, Li Yumei, Zhang Yaqian, Tian Shilong. Regulatory Mechanism of Carvone on Potato Pre‐elite Seed Sprouting via Reactive Oxygen Species and Energy Metabolism[J]. Journal of Henan Agricultural Sciences, 2026, 55(8): 162-171.

李守强, 葛霞, 田甲春, 李梅, 程建新, 李玉梅, 张亚倩, 田世龙. 基于活性氧与能量代谢探究香芹酮对马铃薯原原种发芽的调控机制[J]. 河南农业科学, 2026, 55(8): 162-171.

Figures/Tables 7

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