Cloning，Bioinformatics and Expression Analysis of TaHsc70‑2 Genes in Wheat

doi:10.15933/j.cnki.1004-3268.2026.08.004

Abstract

Abstract: The heat shock protein gene TaHsc70‑2 was cloned from the wheat cultivar Chinese Spring.Subsequently，bioinformatics analysis was performed，and its expression patterns under various hormone treatments［auxin（IAA），gibberellin（GA₃），zeatin（ZT），and abscisic acid（ABA）］and stress conditions（drought，salt，high temperature，and low temperature） were analyzed. The results showed that three TaHsc70‑2 genes，namely TaHsc70‑2‑5A，TaHsc70‑2‑5B，and TaHsc70‑2‑5D，were cloned，which encoded proteins of 648，647，and 648 amino acids，respectively. The gene structure，protein tertiary structure，and conserved motif arrangements and positions of these three genes were highly similar to those of Hsc70‑2 genes in Arabidopsis thaliana and Brachypodium distachyon.The TaHsc70‐2 protein sequences were highly conserved，and showed close phylogenetic relationships with orthologous proteins from Hordeum vulgare and Brachypodium distachyon. Protein‐protein interaction prediction analysis revealed that TaHsc70‐2 proteins had interaction relationships with proteins containing J‐domain and HATPase_c domain. The promoter regions of TaHsc70‑2 genes contained various cis‐acting elements associated with response to hormone，growth and development，and light signaling. TaHsc70‑2 genes were expressed in different tissues at various developmental stages of wheat，with relatively higher expression levels in roots.The expression of TaHsc70‑2 genes was differential under different hormone treatments，and was upregulated under ZT，high temperature，and drought treatments. It is hypothesized that TaHsc70‑2 genes play an important role in wheat growth，development，and responses to abiotic stress.

Key words: Wheat, TaHsc70?2, Cloning, Bioinformatics, Expression analysis, Abiotic stress

摘要： 从小麦品种中国春中克隆热激蛋白基因TaHsc70-2，对其进行生物信息学分析，并分析其在不同激素［生长素（IAA）、赤霉素（GA₃）、玉米素（ZT）和脱落酸（ABA）］和胁迫（干旱、盐、高温、低温）处理下的表达情况。结果表明，共克隆得到3个TaHsc70-2基因：TaHsc70-2-5A、TaHsc70-2-5B、TaHsc70-2-5D，分别编码648、647、648个氨基酸，与拟南芥和二穗短柄草Hsc70-2基因具有相似的基因结构、蛋白质三级结构以及相同的保守基序排列和位置。TaHsc70-2蛋白序列高度保守，与大麦、二穗短柄草同源蛋白亲缘关系较近。蛋白质互作预测分析结果表明，TaHsc70-2蛋白与含J结构域、HATPase_c结构域的蛋白质具有互作关系。TaHsc70-2基因启动子区域包含多种与胁迫、激素、生长发育和光信号响应相关的顺式作用元件。TaHsc70-2基因在小麦不同时期不同组织中均有表达，且在根中的表达量较高。TaHsc70-2基因在不同激素处理下具有不同程度的表达，在ZT、高温和干旱处理下均上调表达。推测TaHsc70-2基因在小麦生长发育及非生物胁迫的响应中起着重要作用.

关键词: 小麦, TaHsc70-2, 克隆, 生物信息学, 表达分析, 非生物胁迫

CLC Number:

S512

Zhang Jun, Wang Haijiang, Zhang Lu, Song Peng, Guo Binbin, Zhou Shuang, Ma Chao. Cloning，Bioinformatics and Expression Analysis of TaHsc70‑2 Genes in Wheat[J]. Journal of Henan Agricultural Sciences, 2026, 55(8): 32-41.

张均, 王海江, 张露, 宋鹏, 郭彬彬, 周爽, 马超. 小麦TaHsc70-2基因的克隆、生物信息学与表达分析[J]. 河南农业科学, 2026, 55(8): 32-41.

Figures/Tables 10

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