Abstract: Abscisic acid（ABA）⁃responsive element（ABRE）binding factor proteins（ABF）constitute a crucial subfamily of bZIP transcription factors that play pivotal roles in both ABA⁃dependent and ABA⁃independent signaling pathways.Through bioinformatics approaches，members of the VvABF family were identified from transcriptome sequencing data of Shine⁃Muscat grape，followed by bioinformatics analysis.Their expression patterns under different storage days and varying chlorine dioxide（ClO₂）concentration treatments were further investigated using qRT⁃PCR analysis.The results showed that the VvABF family comprised thirteen members dividing into three subfamilies and each member contained three to six exons，and the VvABF family members distributed across ten chromosomes with four gene pairs exhibiting collinear relationships.The VvABF family proteins were all hydrophilic and unstable.The entire VvABF family had seven conserved motifs and six conserved structural domains superfamily，which might interact with non⁃specific serine/threonine protein kinases such as D7TPP4_VITVI. Notably，8 g/kg ClO₂treatment significantly increased the expression levels of VvABF2，VvABF4，VvABF9，and VvABF13.ClO₂may mitigate post⁃harvest browning and aging of Shine⁃Muscat grape fruits by increasing the expression levels of VvABF2，VvABF4，VvABF9，and VvABF13genes.

Key words: Shine?Muscat grape, ABF transcription factor, Post?harvest storage quality, ClO₂preservation treatment, Transcriptome sequencing, Bioinformatics analysis

摘要： ABA响应元件结合因子（ABRE binding factor，ABF）是bZIP转录因子的一个重要亚家族，在脱落酸（Abscisic acid，ABA）依赖型和非依赖型信号通路中发挥关键作用。通过生物信息学方法，从阳光玫瑰葡萄转录组测序数据中鉴定VvABF家族成员，对其进行生物信息学分析，并结合qRT-PCR分析其在不同贮藏天数和不同二氧化氯（ClO₂）浓度处理下的表达模式。结果表明，阳光玫瑰葡萄VvABF家族共有13个成员，可分为3个亚家族，每个成员含有3~6个外显子，在10条染色体上均有分布，共4对基因存在共线性关系。VvABF家族蛋白均为亲水蛋白且均不稳定，整个VvABF家族共有7个保守基序和6个保守结构域超家族，可能与非特异性丝氨酸/苏氨酸蛋白激酶如D7TPP4_VITVI等相互作用。此外，8 g/kgClO₂处理下VvABF2、VvABF4、VvABF9和VvABF13表达量显著提高。ClO₂可能通过提高VvABF2、VvABF4、VvABF9和VvABF13基因表达量来减轻采后阳光玫瑰葡萄果实褐变与衰老。

关键词: 阳光玫瑰葡萄, ABF转录因子, 采后贮藏品质, ClO₂保鲜处理, 转录组测序, 生物信息学分析