Abstract: The chromatin remodeling complex SWI/SNF plays an important role in plant growth and development and response to stresses. The characteristics of grape SWI3 gene family were systematically studied by bioinformatics analysis. In this study，four SWI3 gene family members，VvSWI3A，VvSWI3B，VvSWI3C，and VvSWI3D，were identified from Vitis vinifera，which were distributed on three chromosomes of grape.The results of physicochemical properties analysis showed that the four VvSWI3 proteins were acidic hydrophilic proteins with nuclear localization，and α‑helix and random coil accounted for a large proportion（86.59%—94.27%） in the secondary structure. VvSWI3 proteins were divided into four subfamilies based on phylogenetic analysis，each of which contained one member.All VvSWI3 proteins contained SWIRM，SANT，and SWIRM_assoc_1 domains.In addition，VvSWI3D also contained a Zinc finger‑ZZ type domain.The four VvSWI3 genes were similar in structure and contained 5—8 introns.The results of collinearity analysis showed that European grapes had experienced five gene replication events related to the VvSWI3 family during evolution. Different numbers of light，hormone，stress，growth，and development response elements existed in the promoters of the VvSWI3 gene family. It was speculated that the VvSWI3 genes might be involved in the regulation of grape growth and development，stress，and hormone response. It was found that VvSWI3 genes mainly expressed in grape leaves，could respond to drought，low temperature，and high temperature stresses，and showed different expression patterns after ABA，SA，MeJA，and ethephon treatment.

Key words: Grape, SWI3, Bioinformatics, Expression analysis, Stress response

摘要： 染色质重塑复合体SWI/SNF在植物生长发育和胁迫应答中起重要作用。采用生物信息学分析的方法，系统研究了葡萄SWI3基因家族的特性。结果表明，欧洲葡萄中鉴定出4个SWI3基因家族成员，分别为VvSWI3A、VvSWI3B、VvSWI3C 和VvSWI3D，分布在葡萄的3条染色体上。理化性质分析结果显示，4个VvSWI3均属于酸性的亲水蛋白，具有细胞核定位。α-螺旋和无规则卷曲占蛋白质二级结构组成的86.59%~94.27%，为主要的组成结构。系统发育分析将VvSWI3分为4个亚族，每个亚族各包含1个成员。所有VvSWI3均含有SWIRM、SANT和SWIRM_assoc_1结构域，此外，VvSWI3D还额外包含1个ZZ型的锌脂蛋白（Zinc finger-ZZ type）结构域。4个VvSWI3基因结构相似，均含有5~8个内含子。共线性分析结果表明，进化过程中欧洲葡萄共经历了5次与VvSWI3家族有关的基因复制事件。VvSWI3基因家族的启动子区域存在数量不等的光响应、激素响应、胁迫响应及生长发育相关的响应元件，由此推测该家族基因可能参与葡萄生长发育、胁迫及激素响应的调控过程。通过qRT-PCR技术进行的表达分析显示，VvSWI3基因主要在葡萄叶片中表达，且均能对干旱、低温与高温胁迫做出响应；同时，在脱落酸、水杨酸、茉莉酸甲酯及乙烯利处理后，4个VvSWI3基因的表达趋势存在明显差异。

关键词: 葡萄, SWI3, 生物信息学, 表达分析, 胁迫响应