Genetic Diversity Analysis of Local Cowpea Germplasm Resources in Henan Province

doi:10.15933/j.cnki.1004-3268.2025.11.014

Abstract

Abstract: To explore the genetic background and richness of genetic diversity of local cowpea germplasm resources in Henan Province，genetic diversity analysis was conducted on 58 local cowpea germplasm resources using 55 pairs of molecular markers.The results showed that 319 loci were amplified using 55 pairs of molecular markers，including 310 polymorphic loci，with a polymorphism rate of 97.2%.The mean number of observed alleles（Na）was 2.018 2；the mean number of effective alleles（Ne）was 1.763 8；the mean Nei’s gene diversity index（H）was 0.418 1；the mean of Shannon index（I）was 0.608 1；the polymorphic information content（PIC）value was 0. 327 8.The results indicated that the selected primers had good polymorphism and there was good genetic diversity among the local cowpea germplasm resources in Henan Province. Cluster analysis categorized the 58 local long cowpea germplasm resources from Henan Province into population 1 and population 2. All germplasm resources in population 1 originated from the high‑altitude regions of western Henan，while those in Cluster 2 were primarily distributed in low‑altitude areas such as the Nanyang Basin and North China Plain. Molecular markers successfully separated all 58 germplasm resources，but the genetic differentiation between regions was not significant，indicating that local varieties in Henan Province had little relationship with their source regions，and the genetic variation of materials was less affected by local environments.

Key words: Long cowpea, SSR, InDel, Genetic diversity, Cluster analysis

摘要： 为探究河南省地方长豇豆种质资源的遗传背景和遗传多样性丰富程度，使用55对分子标记对58份长豇豆种质资源进行遗传多样性分析。结果表明，使用55对分子标记共扩增出319个位点，多态性位点310个，多态性比率达97.2%；观测等位基因数（Na）均值为2.018 2；有效等位基因数（Ne）均值为1.763 8；Nei’s基因多样性指数（H）均值为0.418 1；香农指数（I）均值为0.608 1；多态信息含量（PIC）均值为0.327 8。说明所选引物具有较好的多态性，河南省地方长豇豆种质资源的遗传多样性较丰富。聚类分析将58份河南省地方长豇豆种质资源分为类群1和类群2，类群1的种质资源全部来自河南省西部高海拔地区；类群2的种质资源主要分布于南阳盆地和华北平原等低海拔地区。使用分子标记可将58份种质资源全部分开，但是各地区之间的遗传分化并不明显，说明河南省地方品种与来源地关系不大，材料的遗传变异受地方环境影响较小。

关键词: 长豇豆, SSR, InDel, 遗传多样性, 聚类分析

CLC Number:

S643.4

BO Xiaopei, LI Wu, WANG Xiaoqing, LÜ Shuping, LI Jingjing, XIAO Yachuan, HUANG Congcong, XU Mingyue, NIE Lihong, ZHAO Fuan, YANG Xiaojie. Genetic Diversity Analysis of Local Cowpea Germplasm Resources in Henan Province[J]. Journal of Henan Agricultural Sciences, 2025, 54(11): 134-143.

薄晓培, 李武, 王晓青, 吕淑平, 李晶晶, 肖亚川, 黄聪聪, 徐铭月, 聂利红, 赵付安, 杨晓杰. 河南省地方长豇豆种质资源的遗传多样性分析[J]. 河南农业科学, 2025, 54(11): 134-143.

Figures/Tables 3

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