Optimization of Wheat Breeding Technology System for Resistance to Fusarium Head Blight in South Huang‑Huai Wheat Region

doi:10.15933/j.cnki.1004-3268.2022.02.004

Abstract

Abstract: In order to further optimize the wheat breeding technology system for resistance to Fusarium head blight（Fhb）and the large‑scale resistance identification technology in the south of Huang‑Huai wheat zone，the wheat varieties with different resistance to Fhb（high resistance，moderate resistance，moderate susceptibility，high susceptibility）were used，the incidence and severity of disease by three frequently used inoculation methods with different inoculation amount were compared under the climatic conditions of the south of Huang‑Huai wheat zone，and limited backcross combined with marker‑assisted selection breeding and haploid breeding were used to select resistant cultivars.The results showed that the optimum inoculation amount for artificial soil surface inoculation method was 120 kg/ha，and the diseased grains should be scattered evenly between wheat rows twice in one month before heading.The optimum inoculation concentration for artificial spray inoculation method was 10⁴/mL，and the conidial suspension should be sprayed on the wheat spike with the amount of 100 mL/m² at the early flowering stage.The optimum inoculation amount for single spikelet inoculation method was 15—20 μL（10⁵/mL），and the conidial suspension should be injected to a spikelet in the middle of spikes at the early flowering stage.The Fhb‑resistant genes Fhb1 and Fhb7 were introduced into the high‑yield wheat cultivar Zhengmai 1860 by the method of limited backcross and marker‑assisted selection，and new wheat lines with better resistance than the recurrent parent were bred based on field resistance identification and agronomic traits selection.By comparing the comprehensive performance of haploid breeding progenies of different types of parents，we considered that it was more easily to breed cultivars with high yield and Fhb resistance using hybrid materials with balance between yield and Fhb resistance.

Key words: Wheat, Fhb（Fusarium head blight）?resistant breeding, Identification technology, Breeding system, South of Huang?Huai wheat zone

摘要： 为了进一步优化黄淮南部麦区小麦抗赤霉病育种技术体系及抗性育种所需的规模化抗性鉴定技术，选择不同赤霉病抗性品种（高抗、中抗、中感、高感品种），在黄淮南部麦区气候条件下对常用的3种人工接种赤霉病菌方法（病粒土表接种、孢子悬浮液喷雾接种、单小花滴注接种）不同接种量的发病效果进行比较，并采用有限回交结合分子标记辅助选择育种技术和单倍体育种技术进行抗赤霉病品种选育。结果表明，病粒土表接种适宜采用的接种量为120 kg/hm²，在小麦抽穗前1个月分2次均匀撒于小麦行间；孢子悬浮液喷雾接种适宜选择孢子悬浮液浓度为10⁴个/mL，在小麦扬花初期以100 mL/m²的喷施量喷于小麦穗部；单小花滴注接种适宜使用15~20 μL浓度为10⁵个/mL的孢子悬浮液，于小麦扬花初期注射到麦穗中部的小穗中。采用有限回交结合分子标记辅助选择育种技术将抗赤霉病基因Fhb1和Fhb7 导入高产小麦品种郑麦1860中，结合田间抗性鉴定和农艺性状选择创制出赤霉病抗性较轮回亲本（郑麦1860）明显提高的小麦新品系。通过比较不同类型亲本的单倍体育种后代，认为在丰产性和抗病性兼顾的材料之间组配杂交组合开展抗赤霉病单倍体育种较易选择出丰抗结合的抗赤霉病品种。

关键词: 小麦, 抗赤霉病育种, 鉴定技术, 育种体系, 黄淮南部麦区

CLC Number:

S512.1

LI Zhengling, ZHANG Yu, HAN Liupeng, WANG Yongxia, FANG Yuhui, HU Lin, XU Weigang. Optimization of Wheat Breeding Technology System for Resistance to Fusarium Head Blight in South Huang‑Huai Wheat Region[J]. Journal of Henan Agricultural Sciences, 2022, 51(2): 28-36.

李正玲, 张煜, 韩留鹏, 王永霞, 方宇辉, 胡琳, 许为钢. 黄淮南部麦区小麦抗赤霉病育种技术体系优化[J]. 河南农业科学, 2022, 51(2): 28-36.

Figures/Tables 8

References

［1］程顺和，张勇，别同德，等. 中国小麦赤霉病的危害及抗性遗传改良［J］.江苏农业学报，2012，28（5）：938‑942.

CHENG S H，ZHANG Y，BIE T D，et al.Damage of wheat Fusarium head blight（FHB） epidemics and genetic improvement of wheat for scab resistance in China［J］.Jiangsu Journal of Agricultural Sciences，2012，28（5）：938‑942.
［2］李韬，李媛媛，李磊.小麦赤霉病：从表型鉴定到抗性改良［J］.科技导报，2016，34（22）：75‑80.
LI T，LI Y Y，LI L.Fusarium head blight in wheat：From phenotyping to resistance improvement ［J］.Science &Technology Review，2016，34（22）：75‑80.
［3］金艳，刘付锁，朱统泉，等.河南省小麦赤霉病的发生情况分析与防治对策［J］.河南科技学院学报（自然科学版），2016，44（6）：1‑4.
JIN Y，LIU F S，ZHU T Q，et al.Occurrence analysis and control measures of wheat scab in Henan Province［J］.Journal of Henan Institute of Science and Technology（Natural Science Edition），2016，44（6）：1‑4.
［4］程顺和，杨士敏，张伯桥，等.小麦对赤霉病的抗扩展性鉴定方法的研究［J］.中国农业科学，1994，27（2）：45‑49.
CHENG S H，YANG S M，ZHANG B Q，et al.Study on the evaluation method of wheat expansion resistance to
Fusarium head blight［J］.Scientia Agricultura Sinica，1994，27（2）：45‑49.
［5］周朝飞，夏穗生，钱存鸣，等.关于小麦抗赤霉病育种问题的探讨［J］.中国农业科学，1987，20（2）：19‑25.
ZHOU C F，XIA S S，QIAN C M，et al.Discussion on breeding of wheat resistance to Fusarium head blight［J］.Scientia Agricultura Sinica，1987，20（2）：19‑25.
［6］余华.小麦抗赤霉病鉴定技术及其在育种中的应用［J］.麦类作物，1997，17（3）：22‑24.
YU H.Identification technology of wheat resistance to Fusarium head blight and its application in breeding［J］.Tritical Crops，1997，17（3）：22‑24.
［7］程顺和，张勇，张伯桥，等.小麦抗赤霉病育种2条技术路线的探讨［J］.扬州大学学报（农业与生命科学版），2003，24（1）：59‑62.
CHENG S H，ZHANG Y，ZHANG B Q，et al.Discussion of two ways of breeding scab resistance in wheat［J］.Journal of Yangzhou University（Agricultural and Life Science Edition），2003，24（1）：59‑62.
［8］许为钢，胡琳，何金江.小麦抗赤霉病育种的实践与探讨［J］.国外农学—麦类作物，1994（5）：33‑35.
XU W G，HU L，HE J J. Practice and discussion on wheat breeding for resistance to Fusarium head blight［J］.Guowai Nongxue：Mailei Zuowu，1994（5）：33‑35.
［9］罗明，蔡华，余飞宇，等.小麦骨干亲本生选6号的赤霉病抗性遗传分析［J］.麦类作物学报，2017，37（12）：1550‑1554.
LUO M，CAI H，YU F Y，et al.Genetic analysis of Fusarium head blight resistance on wheat core parent Shengxuan 6［J］.Journal of Triticeae Crops，2017，37（12）：1550‑1554.
［10］牛皓，姜玉梅，牛吉山.小麦抗赤霉病遗传育种研究进展［J］.农业生物技术学报，2020，28（3）：530‑542.
NIU H，JIANG Y M，NIU J S.Research advances in the genetics and breeding of wheat（Triticum aestivum）
resistance to Fusarium head blight ［J］.Journal of Agricultural Biotechnology，2020，28（3）：530‑542.
［11］张煜，李正玲，王震，等.黄淮南部麦区小麦赤霉病抗性鉴定及基因型分析［J］.麦类作物学报，2020，40（3）：270‑277.
ZHANG Y，LI Z L，WANG Z，et al.Identification the resistance to Fusarium head blight of wheat in the south of Huang‑Huai wheat zone and genotype analysis of resistant cultivars［J］.Journal of Triticeae Crops，2020，
40（3）：270‑277.
［12］顾坚，刘琨，李绍祥，等.昆明自然条件下利用小麦×玉米诱导小麦单倍体的研究初报［J］.麦类作物学报，2006，26（4）：23‑26.
GU J，LIU K，LI S X，et al.Preliminary study on using wheat×maize to induce wheat haploid plant in natural
condition of Kunming［J］.Journal of Triticeae Crops，2006，26（4）：23‑26.
［13］顾坚，杨木军，李绍祥，等.提高小麦×玉米中小麦单倍体苗加倍率的研究［J］.西南农业学报，2012，25（1）：19‑21.
GU J，YANG M J，LI S X，et al.Study on improving frequency of chromosome doubling in wheat haploid produced by wheat×maize system［J］.Southwest China Journal of Agricultural Sciences，2012，25（1）：19‑21.
［14］中华人民共和国农业农村部.小麦抗病虫性评价技术规范：第4部分小麦抗赤霉病评价技术规范：NY/T1443. 4—2007［S］.北京：中国农业出版社，2007.
Ministry Agriculture and Rural Affairs of the People’s Republic of China.Rules for resistance evaluation of wheat to disease and insect pests：Part 4 Rule for resistance evaluation of wheat to wheat scab｛Fusarium graminearum Schwabe ［Teleomorph Gibberella Zese（Schwein）Petch］｝：NY/T 1443. 4—2007［S］.Beijing：China Agricultural Press，2007.
［15］SU Z Q，BERNARDO A，TIAN B，et al.A deletion mutation in TaHRC confers Fhb1 resistance to Fusarium head blight in wheat［J］.Nature Genetics，2019，51（7）：1099‑1105.
［16］LI G Q，ZHOU J Y，JIA H Y，et al.Mutation of a histidine‑rich calcium‑binding‑protein gene in wheat confers resistance to Fusarium head blight［J］.Nature Genetics，2019，51（7）：1106‑1112.
［17］WANG H W，SUN S L，GE W Y，et al.Horizontal gene transfer of Fhb7 from fungus underlies Fusarium head
blight resistance in wheat［J］.Science，2020，368：eaba5435.
［18］SU Z Q，JIN S J，ZHANG D D，et al.Development and validation of diagnostic markers for Fhb1 region，a major QTL for Fusarium head blight resistance in wheat［J］.Theoretical and Applied Genetics，2018，131（11）：2371‑2380.
［19］GUO J，ZHANG X L，HOU Y L，et al.High‑density mapping of the major FHB resistance gene Fhb7 derived from Thinopyrum ponticum and its pyramiding with Fhb1 by marker‑assisted selection［J］.Theoretical and Applied Genetics，2015，128（11）：2301‑2316.

[1]	XU Chunyan, ZHANG Qian, WANG Yaxian, WANG Hongyu, SHENG Kai, WANG Yilun. Effects of Commercial Organic Fertilizers on Physical and Chemical Properties of Sandy Fluvo⁃Aquic Soil，Yield and Nutrient#br# Accumulation of Winter Wheat [J]. Journal of Henan Agricultural Sciences, 2024, 53(2): 56-64.
[2]	WANG Junmei, LI Yahong, XU Fei, YANG Gongqiang, FENG Chaohong, LI Haohai, SONG Yuli. Isolation，Identification of Golubevia albescens 17wy1 and Exploration of Its Control Effect on Wheat Powdery Mildew [J]. Journal of Henan Agricultural Sciences, 2024, 53(2): 101-107.
[3]	MA Zhonglian, GONG jianfu, MA Yongcui, TIAN Hong, LIU Defu, YANG Shunqiang. Effects of Exogenous Melatonin on Physiological Characters，Yield and Quality of Tartary Buckwheat [J]. Journal of Henan Agricultural Sciences, 2024, 53(1): 41-47.
[4]	LIAN Yanhao, DU Feibo, XUE Bo, WANG Zhiqiang, REN Yongzhe, LIN Tongbao, LI Sen. Effect of Bed Planting Combined with One‑time Nitrogen Fertilizer Application on Nitrogen Utilization and Economic Benefit of Winter Wheat（Triticum aestivum L.） [J]. Journal of Henan Agricultural Sciences, 2023, 52(9): 56-65.
[5]	ZHAO Lishang, WANG Xiangsheng, GAO Nanan, HAN Pengbin, LI Jie, REN Yongzhe, LIN Tongbao, WANG Zhiqiang, LIAN Yanhao. Effects of Intercropping of Two Genotypes of Wheat on Grain Filling Characteristics and Yield [J]. Journal of Henan Agricultural Sciences, 2023, 52(8): 18-25.
[6]	LI Haiyong, LI Mengyu, GAO Chuang, XU Hao, YIN Guihong, DONG Chunhao, NIU Jishan, LI Qiaoyun. Correlation Analysis between the Resisitance of Wheat to Black Point and Leaf Blight at Seedling Stage Caused by Bipolaris sorokiniana [J]. Journal of Henan Agricultural Sciences, 2023, 52(8): 96-104.
[7]	HAO Zirui, JIA Yuku, GE Zifei, BI Xintong, ZHANG Jian, XIE Yingxin, WANG Chenyang, MA Dongyun. Quality of Flour and Steamed Bread Made from Black Wheat [J]. Journal of Henan Agricultural Sciences, 2023, 52(8): 163-170.
[8]	ZHANG Keqian, CHENG Gang, WU Wei, SONG Xiangyang, ZHANG Ziqian, YAO Shun, WU Shuai. Extraction of Winter Wheat Planting Area Based on Fused Active and Passive Remote Sensing Images [J]. Journal of Henan Agricultural Sciences, 2023, 52(6): 160-171.
[9]	ZHANG Qiang, WU Limin, LI Pengyan, ZHAO Na, HU Shen’ao, LU Ninghai. Optimization of Fermentation Conditions and Stability Evaluation of Antagonistic Bacteria against Wheat Crown Rot [J]. Journal of Henan Agricultural Sciences, 2023, 52(5): 121-129.
[10]	GUO Fangfang, HAN Yaqian, ZHOU Sumei, DU Xihe, SONG Miao, ZHANG Jiemei, HUANG Yuan, HU Naiyue, ZANG Hecang, YANG Xiwen. Effect of Different N Application Rates on Absorption，Accumulation and Distribution of N and Zn in Different Organs in Wheat at Different Growth Stages [J]. Journal of Henan Agricultural Sciences, 2023, 52(3): 12-25.
[11]	MA Wei, WU Zhiming, YU Kesong. Study on Canopy Chlorophyll Estimation Model of Buckwheat with Different Selenium Levels Based on UAV Multispectrum [J]. Journal of Henan Agricultural Sciences, 2023, 52(3): 161-172.
[12]	QIN Maomao, LIU Yanxi, WANG Wenfei, CHANG Yang, ZHOU Zhengfu, LEI Zhensheng, WU Zhengqing. Quality and Its Stability of Wheat Variety Zhengmai 119 with High Quality [J]. Journal of Henan Agricultural Sciences, 2023, 52(2): 21-30.
[13]	XIN Yujie, LIU Jihong, FENG Dan, ZHAO Guanghua, PEI Jinhua, YIN Haiyan, WANG Yun, WANG Hongqi, LIU Dongmei, CAO Cheng, WANG Junyan, ZHENG Jia, CAO Yingni. Quality Character Analysis of Wheat Varieties（Lines）in Henan Regional Tests from 2017 to 2021 [J]. Journal of Henan Agricultural Sciences, 2023, 52(2): 31-37.
[14]	YANG Chunxia, ZHANG Yan, XIA Xuezhi. Effects of Micro‑fertilizer on Trace Element Content and Yield of Spring Wheat under Foliar Spraying and Drip Irrigation [J]. Journal of Henan Agricultural Sciences, 2023, 52(2): 38-46.
[15]	RONG Yasi, LI Guoqiang, ZHANG Jie, ZHANG Jiantao, WANG Meng, ZHENG Guoqing, FENG Wei. Irrigation Strategies Optimization for Winter Wheat in Henan Province Based on AquaCrop Model [J]. Journal of Henan Agricultural Sciences, 2023, 52(2): 151-161.