Abstract: Virus-induced gene silencing (VIGS) technology,as a means of reverse genetics of plant gene function study,has been widely used in the field of plant functional genomics. VIGS technology is simple,efficient and high-throughput,which is expected to improve the slow state of research on the gene function of pepper,and provide new ideas for the research on gene function and variety improvement of pepper.This paper reviewed the application of VIGS as vector tool in studying gene function related with matter synthesis and accumulation regulation in growth and development,secondary metabolites insect-resistant defense,biotic stress and abiotic stress prevention in pepper. Furtherly,we prospected the development foreground using VIGS technique to explore gene function in pepper. 

Key words: VIGS, Pepper, Gene function, Virus vector, Resistance

摘要： 病毒诱导的基因沉默（Virus-induced gene silencing，VIGS）技术作为植物基因功能研究的反向遗传学手段已被广泛应用于植物功能基因组学研究领域。VIGS技术具有简便、高效和高通量等优点，有望改善辣椒基因功能研究的缓慢状态，为辣椒基因功能研究及品种改良提供新的思路。综述了VIGS载体及其在辣椒上的应用，主要从辣椒生长发育过程中物质合成积累的调控、次生代谢物防虫防病、生物胁迫和非生物胁迫防御等相关基因的功能研究方面进行分析，并对VIGS作为探究辣椒基因功能的有力工具的发展前景进行了展望。

关键词: 病毒诱导的基因沉默, 辣椒, 基因功能, 病毒载体, 抗性