Determination of Herbicide Atrazine Degradation Products by
 Strain Enterobacter sp.and Degradation Gene Cloning

doi:10.15933/j.cnki.1004-3268.2019.10.011

Abstract

Abstract: In order to analyze the biodegradation process of herbicide atrazine，liquid chromatography mass spectrometry was used to detect the degradation products of atrazine by functional strain Enterobacter sp.The degradation gene was cloned by the PCＲ amplification technology,and the degradation gene func tion was detected by using the GO function enrichment analysis.Hydroxyatrazine,cyanuric acid and urea were successively detected in the degradation products，and the degradation genes L465-ＲS09425,L465 ＲS10465,L465-ＲS12445,L465-ＲS09100,L465-ＲS12200 and UreA were cloned.It was speculated that strain Enterobacter sp.could mineralize atrazine gradually by dechlorination,dealkylation,deamination,ring cleavage,decarboxylation and hydrolysis of allophanate under the action of degrading genes．

Key words: Atrazine, Degradation product, Degrading gene, Gene function, Liquid chromatography mass spectrometry, Enterobacter sp．, Herbicide

摘要： 为解析除草剂阿特拉津的生物降解过程，采用液相色谱与质谱联用技术对菌株Enterobacter sp.降解阿特拉津的产物进行检测，通过PCR扩增技术克隆降解基因，并利用GO功能富集分析对降解基因功能进行检测。在降解产物中先后检测出羟基阿特拉津、氰尿酸和尿素组分，克隆出降解基因L465-RS09425、L465-RS10465、L465-RS12445、L465-RS09100、L465-RS12200和UreA。初步推测菌株Enterobacter sp.在降解基因的作用下，经脱氯、脱烷基、脱氨基、环裂解、脱羧和脲基甲酸水解等过程，将阿特拉津逐步矿化。

关键词: 阿特拉津；降解产物；降解基因；基因功能；液质联用；Enterobacter sp., 除草剂

CLC Number:

S182
S154.3

LIU Dandan, SUN Wanyu, LIU Chang, MA Haoke, WANG Ｒuijiao, SUI Yufan. Determination of Herbicide Atrazine Degradation Products by Strain Enterobacter sp.and Degradation Gene Cloning[J]. Journal of Henan Agricultural Sciences, 2019, 48(10): 70-76.

刘丹丹, 孙宛玉, 刘畅, 马浩珂, 王瑞娇, 隋宇凡. 菌株Enterobactersp.降解除草剂阿特拉津产物测定及降解基因克隆[J]. 河南农业科学, 2019, 48(10): 70-76.

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Determination of Herbicide Atrazine Degradation Products by Strain Enterobacter sp.and Degradation Gene Cloning

菌株Enterobactersp.降解除草剂阿特拉津产物测定及降解基因克隆

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