Abstract: To study the effects of Cu²⁺ enrichment on growth of Cordyceps militaris and human health,different concentration of Cu²⁺was added into culture medium to observe the effects of Cu²⁺on the growth of mycelia and fruiting bodies of C.militaris,the content of polysaccharides and Cu²⁺enrichment.The results showed that Cu²⁺inhibited the growth of C.militaris,because the addition of Cu²⁺at the minimum concentration(1.6 mg/L) led to degenerative changes in the fruiting bodies of C.militaris,such as dwarfing and bifurcation.The polysaccharide content of C.militaris increased with the increase of Cu²⁺addition. When the Cu²⁺addition was 3.2 mg/L,the Cu²⁺enrichment rate of mycelium and fruiting body was the highest,54.41% and 191.01% respectively.Therefore,by comparing the above factors,it is found that the optimum Cu²⁺addition is 1.6—3.2 mg/L.With the minimum copper ion addition of 1.6 mg/L, 29 g dry or 116 g fresh fruiting bodies are needed daily to supplement the amount of Cu²⁺needed for people.

Key words: Cordyceps militaris, Cu²⁺tolerance, Growth of mycelia, Growth of fruiting body, Content of polysaccharides, Enrichment characteristic

摘要： 为了解蛹虫草富集铜离子后对其自身生长和人体健康的影响，在培养基中添加不同浓度的铜离子，观察其对蛹虫草菌丝体及子实体生长、多糖含量以及铜离子富集量的影响。结果表明，铜离子对蛹虫草生长有一定抑制作用，最小铜离子添加量（1.6 mg/L）已经导致蛹虫草子实体出现矮小、分叉等退行性变化；蛹虫草的活性成分多糖含量随着铜离子添加量的增加而增加；铜离子添加量为3.2 mg/L时，菌丝体和子实体的铜离子富集率出现高峰值，分别为54.41%和191.01%。综上，最适宜的铜离子添加量为1.6~3.2 mg/L。在最小铜离子添加量1.6 mg/L下，每天食用29 g干蛹虫草或116 g鲜蛹虫草就能补足人体所需铜离子量。

关键词: 蛹虫草, 铜离子耐受性, 菌丝体生长, 子实体生长, 多糖含量, 富集特征