Journal of Henan Agricultural Sciences ›› 2021, Vol. 50 ›› Issue (4): 66-71.DOI: 10.15933/j.cnki.1004-3268.2021.04.009

• Crop Cultivation & Genetic Breeding • Previous Articles     Next Articles

Tolerance and Enrichment Characteristics of Cordyceps militaris to Cu2+

MENG Jiangling,HE De,LI Cuixin   

  1. (College of Life Science,Southwest Forestry University,Kunming 650224,China)
  • Received:2020-11-14 Published:2021-04-15 Online:2021-04-15

蛹虫草对铜离子的耐受性和富集特征

孟江玲,何德,李翠新   

  1. (西南林业大学 生命科学学院,云南 昆明 650224)
  • 通讯作者: 何德(1970-),男,湖南江华人,副教授,博士,主要从事分子细胞生物学研究。E-mail:hedexiner@163.com
  • 作者简介:孟江玲(1995-),女,云南大理人,在读硕士研究生,研究方向:资源微生物开发与利用。E-mail:635553131@qq.com
  • 基金资助:
    云南省优势特色学科生物学一级学科建设项目(50097505);云南省高校林木生物技术重点实验室项目(2018KF19)

Abstract: To study the effects of Cu2+ enrichment on growth of Cordyceps militaris and human health,different concentration of Cu2+was added into culture medium to observe the effects of Cu2+on the growth of mycelia and fruiting bodies of C.militaris,the content of polysaccharides and Cu2+enrichment.The results showed that Cu2+inhibited the growth of C.militaris,because the addition of Cu2+at the minimum concentration(1.6 mg/L) led to degenerative changes in the fruiting bodies of C.militaris,such as dwarfing and bifurcation.The polysaccharide content of C.militaris increased with the increase of Cu2+addition. When the Cu2+addition was 3.2 mg/L,the Cu2+enrichment rate of mycelium and fruiting body was the highest,54.41% and 191.01% respectively.Therefore,by comparing the above factors,it is found that the optimum Cu2+addition is 1.6—3.2 mg/L.With the minimum copper ion addition of 1.6 mg/L, 29 g dry or 116 g fresh fruiting bodies are needed daily to supplement the amount of Cu2+needed for people.

Key words: Cordyceps militaris, Cu2+tolerance, Growth of mycelia, Growth of fruiting body, Content of polysaccharides, Enrichment characteristic

摘要: 为了解蛹虫草富集铜离子后对其自身生长和人体健康的影响,在培养基中添加不同浓度的铜离子,观察其对蛹虫草菌丝体及子实体生长、多糖含量以及铜离子富集量的影响。结果表明,铜离子对蛹虫草生长有一定抑制作用,最小铜离子添加量(1.6 mg/L)已经导致蛹虫草子实体出现矮小、分叉等退行性变化;蛹虫草的活性成分多糖含量随着铜离子添加量的增加而增加;铜离子添加量为3.2 mg/L时,菌丝体和子实体的铜离子富集率出现高峰值,分别为54.41%和191.01%。综上,最适宜的铜离子添加量为1.6~3.2 mg/L。在最小铜离子添加量1.6 mg/L下,每天食用29 g干蛹虫草或116 g鲜蛹虫草就能补足人体所需铜离子量。

关键词: 蛹虫草, 铜离子耐受性, 菌丝体生长, 子实体生长, 多糖含量, 富集特征

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