Abstract: In order to investigate the effect of phoxim on alkaline phosphatase(ALP) activity in different tissues of silkworm,the spectrophotometry was used to detect the changes of ALP activities in fat body,hemolymph,midgut and silk gland of Bombyx mori after feeding phoxim(in the control group sterile water was fed).The results showed that the midgut ALP activity was gradually increased at 6 h after feeding phoxim and reached a maximum value at 15 h,but it decreased sharply and was significantly lower than that of the control group at 24 h.The ALP activities in hemolymph and fat body were increased at 12 h and 15 h respectively after feeding phoxim and both of them reached higher level at 18 h.However,the ALP activity was inhibited at 21 h and 24 h and significantly lower than that of the control group.In silk gland,the ALP activity began to decline at 9 h and continued to decrease with the extension of treatment time.The enzyme activity decreased to 13% of the control group at 24 h and the difference between phoxim treatment group and control group reached an extremely significant level. The above results indicated that the ALP activity was inhibited to a certain extent in the silk gland after treatment with phoxim,while it showed a trend of increasing first and then decreasing sharply in the fat body,hemolymph and midgut of silkworm.It suggests that the ALP in fat body,hemolymph and midgut of silkworm may play an important role in the metabolizing of phoxim.

Key words: Phoxim, Bombyx mori, Alkaline phosphatase, Tissues, Enzymatic activity

摘要： 为了探究辛硫磷对家蚕不同组织碱性磷酸酶（Alkaline phosphatase，ALP）活性的影响，经口喂食辛硫磷（对照组喂食无菌水），采用分光光度法检测家蚕脂肪体、血淋巴、中肠和丝腺ALP活性变化情况。结果显示，中肠组织ALP活性在喂食辛硫磷6 h开始逐渐增加，在15 h达到最高值，随后急剧减弱，在24 h极显著低于对照组。血淋巴ALP活性在12 h开始增加，而脂肪体ALP活性在15 h才开始增加，这2个组织的ALP活性在18 h均较高，在21 h和24 h酶活性被抑制，并且显著低于对照组。丝腺ALP活性在9 h开始下降，并随着处理时间的推移持续减弱，在24 h酶活性降低至对照组的13%，且差异达到极显著水平。综合分析可知，添食辛硫磷，家蚕丝腺ALP活性受到一定程度的抑制，而脂肪体、血淋巴和中肠ALP活性呈现先升高后急剧降低的变化趋势。提示家蚕脂肪体、血淋巴和中肠的ALP在机体代谢辛硫磷过程中发挥了一定的作用。

关键词: 辛硫磷, 家蚕, 碱性磷酸酶, 组织, 酶活性