Journal of Henan Agricultural Sciences ›› 2021, Vol. 50 ›› Issue (3): 91-96.DOI: 10.15933/j.cnki.1004-3268.2021.03.012

• Plant Protection • Previous Articles     Next Articles

Control Effect of Burkholderia pyrrocinia YZU-S377 on Cotton Verticillium Wilt and Its Growth Promotion Function

LIU Lu,WAN Weijie,ZHENG Tongwen,LONG Xinyu,SUN Zhengxiang,ZHOU Yi   

  1. (Agricultural College of Yangtze University/Hubei Provincial Agricultural and Forestry Pest and Disease Early Warning and Regulation Engineering Technology Research Center,Jingzhou 434025,China)
  • Received:2020-07-28 Published:2021-03-15 Online:2021-03-15

根际细菌吡咯伯克霍尔德氏菌YZU-S377对棉花黄萎病的防效及其促生作用研究

刘璐,万伟杰,郑通文,龙欣钰,孙正祥,周燚   

  1. (长江大学 农学院/湖北省农林病虫害预警与调控工程技术研究中心,湖北 荆州 434025)
  • 通讯作者: 孙正祥(1980-),男,湖北黄冈人,副教授,博士,主要从事植物病害生物防治研究。E-mail: sunzhengxiang9904@126.com 周燚(1972-),男,湖北钟祥人,教授,博士,主要从事分子植物病理学研究。E-mail:zhouyi@yangtzeu.edu.cn
  • 作者简介:刘璐(1994-),女,在读硕士研究生,研究方向:植物病理学。E-mail:13468786908@163.com
  • 基金资助:
    湖北省教育厅重点项目(D20181304)

Abstract: Burkholderia pyrrocinia YZU-S377(S377) is a rice rhizosphere bacterium obtained in the early stage,which has broad-spectrum inhibitory effect on plant pathogens.To verify the biocontrol potential of S377 against Verticillium dahliae,the control effect of S377 on cotton Verticillium wilt,the colonization dynamics and growth promoting effect of S377 on cotton were tested in pot. The results showed that the relative inhibition rate of the strain to V.dahliae was 89.22%,and the control effect of pot test was 62.53%.After treated with S377 sterile filtrate the germination rate of cotton increased by 22.22 percentage points,the root length of cotton seedlings increased by 27.78 mm,and the fresh weight of aboveground part increased by 0.73 g.The results of crystal violet staining showed that strain S377 had the ability to form biofilm.The biofilm formation intensity of strain S377 at OD600=0.15 and 0.25 was significantly more obvious than that under other concentrations.The colonization experiment showed that S377 could colonize cotton roots for 21 days,and the number increased first and then decreased. The
results indicated that S377 was a potential biocontrol strain against cotton Verticillium wilt.

Key words: Burkholderia pyrrocinia;Verticillium dahliae;Biological control;Pot experiment;Growth promotion, Colonization dynamics

摘要: 吡咯伯克霍尔德氏菌(Burkholderia pyrrocinia)YZU-S377(S377)是前期获得的1株水稻根际细菌,对植物病菌具有广谱抑制作用。为探究S377对棉花黄萎病的生防潜力,测定了菌株S377对棉花黄萎病的盆栽防效、在棉花植株内的定殖动态及其促生作用。结果表明,菌株S377对棉花黄萎病菌(Verticillium dahliae)的相对抑制率为89.22%,盆栽防效为62.53%;经S377无菌滤液处理后,棉花种子发芽率提高了22.22个百分点,棉花幼苗根长增加了27.78 mm,地上鲜质量增加了0.73 g;菌株S377可以形成生物被膜,在OD600等于0.15和0.25时形成生物被膜强度较显著,在棉花植株根部可以持续定殖21 d,其数量呈先增后减趋势。上述结果表明,菌株S377对棉花黄萎病具有良好的生防潜力。

关键词: 吡咯伯克霍尔德氏菌, 棉花黄萎病菌, 生物防治, 盆栽防效, 促生作用, 定殖动态

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