Genome‐Wide Identification and Expression Analysis of ASR Gene Family in Main Gramineae Crops

doi:10.15933/j.cnki.1004-3268.2021.12.002

Abstract

Abstract: The abscisic acid‐，stress‐，ripening‐induced protein（ASR） family genes in millet，rice，sorghum，maize and wheat were identified，and their sequences，phylogeny，structural domains，promoters and expression patterns were analyzed，so as to provide references for further study of ASR protein function. The results showed that 7，6，8，9 and 29 ASR genes were identified in millet，rice，sorghum，maize and wheat respectively，which was unevenly distributed on the chromosomes. ASR family genes were clustered into 4 subgroups，and the gene structure was conserved in the same group，diverse among groups.ASR protein had conserved amino acid motifs，but subgroup Ⅱ‐2 differed significantly compared with the other subgroups，and all ASR family members contained only the ABA/WDS domain，which was located at the C‐terminal. As a whole，ASR genes were undergoing purifying selection during evolution.Subcellular localization results showed that most ASR proteins were localized in the nucleus，and a fraction was localized in the cytoplasm.The results of promoter analysis indicated that ASR family genes had a large number of tissue‐specific elements，stress‐responsive elements and hormone‐responsive elements.Expression analysis results showed that ASR genes responded extensively to abiotic stresses，had tissue‐specific expression characteristics，and the expression patterns differed among species and genes.

Key words: Gramineae crops, ASR gene family, Expression analysis, Phyletic evolution

摘要： 对谷子、水稻、高粱、玉米、小麦等主要禾本科作物ASR（Abscisic acid‐，stress‐，ripening‐induced protein）家族基因进行鉴定，并对其序列、系统进化、结构域、启动子及表达模式进行分析，为进一步研究ASR蛋白功能提供参考。结果表明，从谷子、水稻、高粱、玉米、小麦基因组中分别鉴定出7、6、8、9、29个ASR家族成员，不均匀分布在染色体上。ASR家族基因聚类为4个亚组，基因结构具有组内保守性和组间多样性。ASR蛋白具有保守的氨基酸基序，但Ⅱ-2亚组相较于其他亚组差异明显。ASR家族成员均只含有ABA/WDS结构域，位于C-末端。在进化过程中，ASR基因整体上正经历纯化选择。亚细胞定位预测结果表明，大部分ASR蛋白定位在细胞核，小部分定位在细胞质。启动子分析结果表明，ASR家族基因具有大量组织特异性元件、应激响应元件和激素响应元件。表达分析结果表明，ASR基因广泛响应非生物胁迫，其表达具有组织特异性，并且不同物种、不同基因间存在差异。

关键词: 禾本科作物, ASR基因家族, 表达分析, 系统进化

CLC Number:

MENG Yaxuan, SUN Yingqi, ZHAO Xinyue, WANG Fengxia, WENG Qiaoyun, LIU Yinghui. Genome‐Wide Identification and Expression Analysis of ASR Gene Family in Main Gramineae Crops[J]. Journal of Henan Agricultural Sciences, 2021, 50(12): 10-22.

孟亚轩, 孙颖琦, 赵心月, 王凤霞, 瓮巧云, 刘颖慧. 主要禾本科作物ASR 基因家族全基因组鉴定及表达分析[J]. 河南农业科学, 2021, 50(12): 10-22.

Figures/Tables 6

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