Abstract: In order to investigate the correlation between ORAI1 gene and eggshell quality of Sansui duck,a DNA mixing pool was constructed with Sansui duck as the research object.The ORAI1 gene of Sansui duck was detected for single nucleotide polymorphism(SNP) using PCR amplification and direct sequencing,which had significant effect on eggshell quality of Sansui duck.At the same time,the function of ORAI1 protein in Sansui duck was predicted.The results showed that ORAI1 gene of Sansui duck encoded 263 amino acids,which constituted an unstable hydrophilic protein.The secondary structure of ORAI1 protein consisted mainly of α helix,random curl,extension chain and β trun.After PCR amplification,three SNPs were found in the amplified segment,namely T84C,C188T and A471G.Synonymous mutation did not cause the change of amino acid,but caused the change of mRNA secondary structure and free energy of the gene,and the allele frequencies of each mutation site were different before and after mutation.The results indicated that there were three SNPs in ORAI1 gene,which could be used as a reference for molecular marker sites to improve eggshell quality.

Key words: Sansui duck, ORAI1 gene, SNP, Protein function prediction, Eggshell quality

摘要： 为探讨钙释放酶激活钙调制器1（ORAI1）与三穗鸭蛋壳品质的相关性，构建三穗鸭DNA混合池，运用PCR扩增和直接测序法对三穗鸭ORAI1基因进行单核苷酸多态性（SNP）检测，进而筛选出对三穗鸭蛋壳品质有显著影响的SNPs，同时对三穗鸭ORAI1基因编码的蛋白质进行功能预测。结果显示，三穗鸭ORAI1基因编码263个氨基酸，组成一种不稳定的亲水性蛋白，蛋白质的二级结构主要由α螺旋、无规卷曲、延伸链和β转角构成；在扩增段共发现3个SNPs，分别是T84C、C188T、A471G，均为同义突变，未造成氨基酸的改变，但引起该基因mRNA二级结构和自由能的变化，并且各突变位点在突变前后等位基因频率存在差异。以上结果提示，ORAI1基因有3个SNPs，可作为改善蛋壳品质分子标记位点的参考。

关键词: 三穗鸭, ORAI1基因, SNP, 蛋白质功能预测, 蛋壳品质