Analysis on the Polymorphism and Tissue Expression of ANXA2 Gene in Pigs

doi:10.15933/j.cnki.1004-3268.2022.02.014

Abstract

Abstract: In order to explore the single nucleotide polymorphism（SNP）of Annexin A2（ANXA2）gene and its expression differences in tissues，180‑day‑old Tibetan pigs and Yorkshire pigs were used as experimental objects to detect the mRNA expression difference of ANXA2 gene in liver，abdominal fat and dorsal fat tissus，and the polymorphism of 5′flanking region of ANXA2 gene was analyzed. The results showed that four SNP sites were screened in the 5′flanking region of ANXA2 gene，g.111574291G>A，g.111574499C>T，g.111574522G>C and g.111574638A>G. There was a significant difference in allele frequency between Tibetan pigs and Yorkshire pigs（P<0. 05）.In liver tissue，the mRNA expression trend of ANXA2 gene in Tibetan pigs and Yorkshire pigs was basically the same；In abdominal fat and dorsal fat tissus，the mRNA expression of ANXA2 gene in Tibetan pigs was significantly lower than that in Yorkshire pigs（P<0. 01）.In conclusion，ANXA2 gene may be an important candidate gene for regulating fat deposition and fat metabolism.

Key words: ANXA2 gene, Tibetan pig, Yorkshire pig, Tissue expression, SNP

摘要： 为了探究膜联蛋白A2(ANXA2)基因单核苷酸多态性（SNP）及其在组织中的表达差异，以180日龄的藏猪、大约克猪作为试验对象，检测ANXA2 基因在肝脏、腹脂和背脂组织上mRNA表达差异，并对ANXA2基因的5′侧翼区进行多态性分析。结果显示，在ANXA2基因5′侧翼区筛选到4个SNP位点，g.111574291G> A、g.111574499C>T、g.111574522G>C和g.111574638A>G，且藏猪与大约克猪等位基因频率差异显著（P<0.05）。在肝脏中，藏猪和大约克猪ANXA2基因的mRNA表达趋势基本一致；在背脂和腹脂中，藏猪ANXA2基因的mRNA表达量极显著低于大约克猪（P<0.01）。综上，ANXA2基因可能是调控脂肪沉积和脂肪代谢的重要候选基因。

关键词: ANXA2 基因, 藏猪, 大约克猪, 组织表达, 单核苷酸多态性

CLC Number:

S828

LU Hong, LI Xindi, WANG Yu, LUOBU Baizhen, DUAN Mengqi, YE Yourong, ZHANG Jian, SHANG Peng. Analysis on the Polymorphism and Tissue Expression of ANXA2 Gene in Pigs[J]. Journal of Henan Agricultural Sciences, 2022, 51(2): 120-126.

卢鸿, 李新荻, 王宇, 罗布白珍, 段梦琪, 叶幼荣, 张健, 商鹏. 猪ANXA2 基因多态性及组织表达差异分析[J]. 河南农业科学, 2022, 51(2): 120-126.

Figures/Tables 6

References

［1］陈晨，胡雄贵，朱吉，等.猪脂肪发育相关miRNAs的功能研究进展［J］.畜牧兽医学报，2015，46（12）：2117‑2126.
CHEN C，HU X G，ZHU J，et al.Progress on the research of miRNAs associ‑ated with fat development in pigs［J］.Acta Veterinary Et Zootechnica Sinica，2015，46（12）：2117‑2126.
［2］杨永玉，胡长平.肥胖与脂肪组织重构［J］.中国药理学通报，2016，32（1）：9‑13.
YANG Y Y，HU C P.Obesity and adipose tissue remodeling［J］.Chinese Phar‑macological Bulletin，2016，32（1）：9‑13.
［3］GABEL M，ROYER C，THAHOULY T，et al.Annexin A2 egress during calcium‑regulated exocytosis in neuroendocrine cells［J］.Cells，2020，9（9）：2059.
［4］HAYES M J，SHAO D M，BAILLY M，et al.Regulation of actin dynamics by annexin 2［J］.The EMBO J，2006，25（9）：1816‑1826.
［5］SECKINGER A，MEIβNER T，MOREAUX J，et al.Clinical and prognostic role of annexin A2 in multiple myeloma［J］.Blood，2012，120（5）：1087‑1094.
［6］IACCARINO L，GHIRARDELLO A，CANOVA M，et al.Anti‑annexins autoantibodies：Their role as biomarkers of autoimmune diseases［J］.Autoimmun Rev，2011，10（9）：553‑558.
［7］GREWAL T，RENTERO C，ENRICH C，et al.Annexin animal models：From fundamental principles to translational research［J］.Int J Mol Sci，2021，22（7）：3439.
［8］MANUEL T J，LORENA R M，ESTELA M P. Annexin A2 and S100A10 in the mammalian oviduct［J］.Cell Tissue Res，2016，363（2）：567‑577.
［9］王言研，王洋洋，李佩玲.膜联蛋白A2在癌症发生和进展中的作用［J］.肿瘤学杂志，2014，20（11）：951‑954.
WANG Y Y，WANG Y Y，LI P L.The role of annexin A2 in carcinogenesis and progression of cancer［J］.Journal of
Chinese Oncology，2014，20（11）：951‑954.
［10］王增增，徐婷，吴敏.膜联蛋白A2在自身免疫性疾病中的研究进展［J］.中国全科医学，2018，21（36）：4537‑4540.
WANG Z Z，XU T，WU M.Developments of ANXA2 in autoimmune diseases［J］.Chinese General Practice，2018，21（36）：4537‑4540.
［11］李辰跃，顾操，赵世红.膜联蛋白A2磷酸化与功能调控研究进展［J］.第二军医大学学报，2019，40（2）：213‑218.
LI C Y，GU C，ZHAO S H.Advance on phosphorylation and function regulation of annexin A2［J］.Academic Journal of Second Military Medical University，2019，40（2）：213‑218.
［12］付睿琦.利用蛋白质组学技术研究北京油鸡肌肉发育和肌内脂肪沉积的分子机制［D］.北京：中国农业科学院，2013.
FU R Q.Study on the molecular mechanism of muscle development and intramuscular fat deposition in Beijing
you chicken by proteomics［D］.Beijing：Chinese Academy of Agricultural Sciences，2013.
［13］周扬.秦川牛脂肪沉积相关基因筛选及可变剪接对基因表达和细胞定位的影响研究［D]. 杨凌：西北农林科技大学，2017.
ZHOU Y.Screening of genes related to fat deposition and the effect of alternative splicing on gene expression and cell localization in Qinchuan cattle［D］.Yangling：Northwest A & F University，2017.
［14］LEGGATE M，CARTER W G，EVANS MATTHEW J C，et al.Determination of inflammatory and prominent proteomic changes in plasma and adipose tissue after high‑intensity intermittent training in overweight and obese males［J］.J Appl Physiol（1985），2012，112（8）：1353‑1360.
［15］SALAMEH A，DAQUINAG A C，STAQUICINI D I，et al.Prohibitin/annexin 2 interaction regulates fatty acid transport in adipose tissue［J］.JCI Insight，2016，1（10）：86351.
［16］HUANG J，HSIA S H，IMAMURA T，et al.Annexin Ⅱ is a thiazolidinedione‑responsive gene involved in insulin‑induced glucose transporter isoform4 translocation in 3T3‑L1 adipocytes［J］.Endocrinology，2004，145（4）：1579‑1586.
［17］TEIJEIRO J M，IGNOTZ G G，MARINI P E.Annexin A2 is involved in pig （Sus scrofa）sperm‑oviduct interaction［J］.Mol Reprod Dev，2009，76（4）：334‑341.
［18］LI J N，GUO D W，HUANG L，et al.The interaction between host annexin A2 and viral Nsp9 is beneficial for replication of porcine reproductive and respiratory syndrome virus［J］.Virus Research，2014，189（1）：106‑113.
［19］赵彦玲，李善政，王福成，等.藏猪甲基转移酶3a基因的SNP筛选及其在精子中的表达分析［J］.河南农业科学，2020，49（10）：156‑161.
ZHAO Y L，LI S Z，WANG F C，et al.SNP screening of DNA methyltransferase 3a gene in tibetan pig and its expression in spermatozoa ［J］.Journalof Henan Agricultural Sciences，2020，49（10）：156‑161.
［20］强巴央宗，张浩，纪素玲，等.藏猪屠宰性能和肉质测定与分析［J］.中国畜牧杂志，2008，44（21）：10‑11，48.
QIANG BA Y Z，ZHANG H，JI S L，et al.Analysis of slaughter performance and meat quality of Tibetan pigs［J］.Chinese Journal of Animal Science，2008，44（21）：10‑11，48.
［21] AZAIN M J.Role of fatty acids in adipocyte growth and development［J］.J Anim Sci，2004，82（3）：916‑924.
［22］WOOD J D，RICHARDSON R I，NUTE G R，et al.Effects of fatty acids on meat quality：A review［J］.Meat Sci，2004，66（1）：21‑32.
［23］TANG J，YANG L R，LI Y F，et al.ARID3A promotes the development of colorectal cancer by upregulating AURKA［J］.Carcinogenesis，2021，42（4）：578‑586.
［24］LAURA H，ALAIN N. CUX1 transcription factors：From biochemical activities and cell‑based assays to mouse
models and human diseases［J］.Gene，2012，497（1）：18‑26.
［25］BEATRIZ C，ALVARO S S，LEONARDO B，et al.Cux1 and Cux2 regulate dendritic branching，spine morphology，and synapses of the upper layer neurons of the cortex［J］.Neuron，2010，66（4）：523‑535.
［26］谢铖，徐安琪，韩磊，等.CUX1及其分子调控机制在人类恶性肿瘤中的研究进展［J］.分子影像学杂志，2019，42（3）：343‑348.
XIE C，XU A Q，HAN L，et al.Advances in the study of CUX1 and its molecular regulatory mechanisms in human malignant tumors［J］.Journal of Molecular Imaging，2019，42（3）：343‑348.
［27］STRATIGOPOULOS G，LEDUC C A，CREMONA M L，et al.Cut‑like homeobox 1 （CUX1） regulates expression of the fat mass and obesity‑associated and retinitis pigmentosa gTPase regulator‑interacting protein‑1‑like（RPGRIP1L） genes and coordinates leptin receptor signaling［J］.Journal of Biological Chemistry，2011，286（3）：2155‑2170.
［28］DONGKYOON K，CHRISTIAN S，MARK B，et al.Competitive promoter‑associated matrix attachment region binding of the Arid3a and Cux1 transcription factors ［J］.Multidisciplinary Digital Publishing Institute，2017，5（4）：34.
［29］岳海清，周后德.白色脂肪组织在不同类型肥胖和代谢状态人群中的差异及作用［J］.临床与病理杂志，2021，41（6）：1448‑1453.
YUE H Q，ZHOU H D.Difference and effect of white adipose tissue in different types of obesity and metabolic
status［J］.International Journal of Pathology and Clinical Medicine，2021，41（6）：1448‑1453.
［30］YONG M Z，URMILA B，MICHAEL V D，et al.Proteome differences associated with fat accumulation in bovine subcutaneous adipose tissues［J］.BioMed Central，2010，8（1）：14.
［31］PIRAS C，MORITTU V M，SPINA A A，et al.Unraveling the adipose tissue proteome of transition cows through severe negative energy balance［J］.Animals（Basel），2019，9（12）：1013.
［32］FILIPENKO N R，MACLEOD T J，YOON C S，et al.Annexin A2 is a novel RNA‑binding protein［J］.J Biol Chem，2004，279（10）：8723‑8731.

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