Abstract: The homologous gene of Arabidopsis thaliana CER4 regulating wax synthesis and metabolism was cloned from South China cucumber 649 and named as CsCER4.The CsCER4 gene had a CDS region with 540 bp in length and encoded 179 amino acids.Promoter sequence analysis indicated that the CsCER4 promoter had regulatory elements related to various stresses such as drought,cold,and salt.Phylogenetic analysis showed that cucumber CsCER4 was closely related to CER4 of melon,bitter gourd,pumpkin and zucchini.The results of qRT-PCR showed that CsCER4 had high expression level in ovary and pericarp,and
its expression level was low in male flowers,stems and leaves.It was speculated that CsCER4 might directly participate in or affect the synthesis of wax by regulating the wax synthesis pathway.

Key words: Cucumber, Epidermis wax, CsCER4, Expression analysis

摘要： 以华南型黄瓜649为材料，克隆了拟南芥调控蜡质合成基因CER4在黄瓜中的同源基因，命名为CsCER4。CsCER4基因CDS全长540 bp，编码179个氨基酸。启动子序列分析表明，CsCER4 启动子中包括与干旱、冷、盐等多种逆境胁迫相关的调控元件。进化分析表明，黄瓜CsCER4与同为葫芦科的甜瓜、苦瓜、南瓜和西葫芦的CER4亲缘关系较近。qRT-PCR 结果表明，CsCER4 在黄瓜的子房和果皮中表达量较高，而在雄花、茎和叶片中表达量较低。推测CsCER4可能直接参与或通过调控蜡质合成途径影响蜡质的合成。

关键词: 黄瓜, 表皮蜡质, CsCER4, 表达分析