doi:10.15933/j.cnki.1004-3268.2018.02.004

Abstract

摘要： 为探索适用于双向电泳分析的玉米组织全蛋白质提取的最佳方法，建立适用于玉米组织的全蛋白质双向电泳体系，以玉米叶片和花粉为材料，在相同条件下采用PDQuest分析，分别比较了丙酮预处理和TCA/丙酮预处理玉米组织对双向电泳图谱的影响。结果表明，丙酮预处理和TCA/丙酮2种预处理提取法在玉米叶片中分别检测到634个和540个清晰的蛋白质点，在玉米花粉中分别检测到410个和320个清晰的蛋白质点。丙酮预处理的玉米组织有少量拖尾，蛋白质点丰度高，适合做质谱分析；TCA/丙酮预处理的玉米组织拖尾较轻，蛋白质点丰度较低，适合做蛋白质差异分析。

关键词: 预处理, 玉米, 叶片, 花粉, 蛋白质, 丙酮, 三氯乙酸（TCA）/丙酮

CLC Number:

S513

安素妨,侯锦娜,王艳,鲁丹丹,李保全. 2种预处理对玉米组织全蛋白质双向电泳图谱的影响[J]. 河南农业科学, 2018, 47(2): 17-21.

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2种预处理对玉米组织全蛋白质双向电泳图谱的影响

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