doi:10.3969/j.issn.1004-3268.2000.12.013

Abstract

摘要： 将鸡传染性支气管炎病毒Ｍ４１株尿囊液差速离心纯化，制备抗原；应用杂交瘤技术建立了３株分泌抗鸡传染性支气管炎病毒的杂交瘤细胞株，其免疫球蛋白亚类分别属于ＩｇＧ１、ＩｇＧ２ａ、ＩｇＧ２ｂ。３株单克隆抗体与鸡新城疫病毒、传染性法氏囊病病毒、产蛋下降综合征病毒及禽流感病毒均无交叉反应，ＹＮＺ－５５和ＹＮＺ－６２可识别多数鸡传染性支气管炎标准毒株及地方分离株，其腹水ＥＬＩＳＡ效价达１０＾－５，且识别的抗原位

关键词: 鸡, 传染性支气管炎病毒, 单克隆抗体, 早期诊断

CLC Number:

李青梅;张改平. 抗鸡传染性支气管炎病毒单克隆抗体的制备与应用[J]. 河南农业科学, 2000, 29(12): 26-28.

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抗鸡传染性支气管炎病毒单克隆抗体的制备与应用

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