Abstract: To establish an efficient and stable in vitro regeneration system for Aster tataricus L.F.，leaf and flower bud explants were utilized to evaluate the effects of hormone combinations ［6‑BA（6‑benzylaminopurine），NAA（1‑naphthaleneacetic acid），2，4‑D（2，4‑dichlorophenoxyacetic acid），KT（kinetin）］ and low‑temperature（4 ℃ ） pretreatment on callus induction and adventitious bud differentiation. Furthermore，the regulatory effects of sucrose concentration and moderate dehydration treatment on vitrified plantlets were systematically analyzed.The results showed that the optimal disinfection protocol for Aster tataricus L.F.explants was immersion in 0.1% HgCl_₂ for 8 minutes.Leaf explants could induce callus formation but failed to differentiate into adventitious buds，while flower buds were identified as suitable explants for constructing an efficient regeneration system.Among these，4 mm flower buds at the mid‑flowering stage were optimal for callus induction，and the optimal medium for both callus induction and adventitious bud differentiation was MS+6‑BA 1.5 mg/L.The optimal medium for adventitious bud proliferation was MS+6‑BA 1. 0 mg/L. Compared with ambient temperature culture，low‑temperature pretreatment at 4 ℃ for 7 days significantly enhanced the callus induction rate.Agar，sucrose，PEG‑6000，and dehydration treatments could all improve the status of vitrified plantlets to varying extents，with the best effect achieved by 50 g/L sucrose treatment，and the recovery rate of vitrified seedlings reaching 60.00%.In conclusion，this study clarified the suitable explant（4 mm flower buds at the mid‑flowering stage），optimal explant disinfection protocol（0.1% HgCl_₂ treatment for 8 minutes），and medium formulations for regeneration system establishment（callus induction and adventitious bud differentiation：MS+6‑BA 1.5 mg/L；adventitious bud proliferation：MS+6‑BA 1.0 mg/L），as well as effective regulatory measures（4 ℃ low‑temperature pretreatment for 7 days to promote callus induction，and 50 g/L sucrose to mitigate vitrification）.An efficient and stable in vitro regeneration system for Aster tataricus L.F.was successfully established.

Key words: Aster tataricus L.F., Flower bud, Plant hormone, Regeneration system, Vitrified seedling, Regulation technique

摘要： 为建立高效稳定的紫菀（Aster tataricus L.F.）离体再生体系，以紫菀叶片和花蕾为外植体，研究了激素［6-BA（6-苄氨基嘌呤）、NAA（萘乙酸）、2，4-D（2，4-二氯苯氧乙酸）、KT（激动素）］组合、低温（4 ℃）预处理对愈伤组织诱导和不定芽分化的影响，并分析蔗糖质量浓度及适度干燥（脱水）处理对玻璃化试管苗的调控效果。结果表明，紫菀外植体最佳消毒方案为0.1% HgCl2消毒8 min；叶片外植体可诱导形成愈伤组织，但无法分化不定芽，花蕾是构建紫菀高效再生体系的适宜外植体，其中开花中期4 mm的花蕾为愈伤组织诱导的最适外植体，其最适诱导培养基及不定芽分化培养基为MS+6-BA 1.5 mg/L，不定芽增殖最适培养基为MS+6-BA 1.0 mg/L；与常温培养相比，4 ℃低温预处理7 d 可显著提升愈伤组织诱导率；琼脂、蔗糖、PEG-6000及脱水处理均能不同程度改良玻璃化苗状态，以50 g/L 蔗糖处理效果最佳，玻璃化苗恢复率达60.00%。综上，明确了紫菀离体再生的适宜外植体（开花中期4 mm 花蕾）、最优外植体消毒方案（0.1% HgCl₂消毒8 min）、再生体系建立培养基配方（愈伤诱导及不定芽分化：MS+6-BA1.5 mg/L；不定芽增殖：MS+6-BA 1.0 mg/L）、有效调控措施（4 ℃低温预处理7 d 提升愈伤诱导率、50 g/L蔗糖处理调控玻璃化苗），构建了高效稳定的紫菀离体再生体系。

关键词: 紫菀, 花蕾, 植物激素, 再生体系, 玻璃化苗, 调控技术