Abstract: To investigate the metabolic properties and gene expression regulation of the parental and hybrid versions of Flammulina velutipes，the variety FY5 and the hybrid generation F₁ of its cross with the wild type as materials were used for transcriptomics and metabolomics analyses. The results showed that a total of 128 differential metabolites were identified，and those with large differences included organic acids，amino acids，ketones，unsaturated fatty acids and polysaccharides.Eighty‑seven of them were significantly up‑regulated and 41 were significantly down‑regulated in the hybrids.A total of 3 329 significantly differentially expressed genes，2 389 up‑regulated differentially expressed genes and 940 down‑regulated differentially expressed genes were detected.The joint analysis showed that the significantly differentially expressed genes and differential metabolites mainly existed in eight metabolic pathways，including glutathione metabolism and arginine synthesis.

Key words: Flammulina velutipes, Transcriptomics, Metabonomics, Hybrid advantage, Differential analysis

摘要： 为了探究金针菇亲本与杂交本代谢特性与基因表达调控，以金针菇品种FY5及其与野生型杂交的杂种一代F₁为材料，进行转录组学和代谢组学分析。结果表明，共鉴定差异代谢物128种，差异较大的包括有机酸类、氨基酸类、酮类、不饱和脂肪酸和糖类。有87种在杂交本中显著上调，41种显著下调。共检测到显著差异基因3 329个，上调差异基因2 389个，下调差异基因940个。联合分析表明，显著差异表达基因和差异代谢物主要存在于谷胱甘肽代谢、精氨酸合成等8条代谢途径。

关键词: 金针菇, 代谢组学, 转录组学, 杂种优势, 差异分析