Effects of Kidney Bean Resistant and Susceptible Varieties on Growth and Development and Metabolic Enzymes of Aphis craccivora Koch.

doi:10.15933/j.cnki.1004-3268.2026.02.011

Abstract

Abstract: In order to explore the effects of kidney bean resistant and susceptible varieties on the growth and development of Aphis craccivora，the activities of digestive enzymes（lipase，protease，α‑amylase）and detoxification enzymes（glutathione‑S‑transferase，carboxylesterase，acetylcholinesterase）in the body of A.craccivora fed on kidney bean resistant and susceptible varieties were determined，and the life table of A.craccivora population continuously reproducing for three generations on kidney bean resistant and susceptible varieties was constructed.The results showed that the activities of three digestive enzymes，carboxylesterase and acetylcholinesterase in A.craccivora after feeding on susceptible kidney bean varieties were significantly higher than those of A. craccivora after feeding on resistant varieties，but the activity of glutathione‑S‑transferase in A.craccivora after feeding on resistant varieties was significantly higher than that on susceptible varieties，and the increase was 12.9%.The survival rate of F₂ and F₃ generations of A.craccivora parasitized on aphid‑resistant varieties decreased compared with F₁ generation，and the number of A.craccivora gradually decreased，while those on the susceptible varieties were the opposite.The net value‑added rate（R₀）of the F₃ generation on the susceptible variety was 28.60，while that on the resistant variety was only 11.13，and there was a significant difference.In conclusion，after feeding on the insect‑resistant kidney bean variety，the activities of digestive enzymes，carboxylesterase and acetylcholinesterase in the bodies of A.craccivora decreased，while the activity of glutathione‑S‑transferase increased，and the survival rate of their offspring and the number of aphids produced decreased.However，the opposite was true when they fed on the susceptible kidney bean varieties.

Key words: Kidney bean, Aphis craccivora, Life table, Digestive enzymes, Detoxifying enzymes

摘要： 为了探究芸豆抗、感品种对豆蚜生长发育的影响，测定了豆蚜取食芸豆抗、感品种后体内消化酶（脂肪酶、蛋白酶、α-淀粉酶）和解毒酶（谷胱甘肽-S-转移酶、羧酸酯酶、乙酰胆碱酯酶）活性的变化，并构建了芸豆抗虫、感虫品种连续繁衍3代的豆蚜种群生命表。结果表明，豆蚜取食感虫芸豆品种后体内3种消化酶、羧酸酯酶以及乙酰胆碱酯酶活性均极显著高于取食抗虫品种后的豆蚜个体，但豆蚜取食抗虫品种后体内的谷胱甘肽-S-转移酶活性极显著高于感虫品种，增幅为12.9%。寄生于抗蚜品种上的豆蚜F₂、F₃代存活率相比F₁代有所下降，产蚜量也逐渐降低，感虫品种则相反。感虫品种日本红上豆蚜F₃代的净增殖率（R₀）为28.60，而抗虫品种仅为11.13，存在极显著差异。综上，豆蚜取食芸豆抗虫品种后体内的消化酶、羧酸酯酶以及乙酰胆碱酯酶活性降低，谷胱甘肽-S-转移酶活性提高，后代存活率和产蚜量降低，而取食芸豆感虫品种后则相反。

关键词: 芸豆, 豆蚜, 生命表, 消化酶, 解毒酶

CLC Number:

S433.1

ZHU Yuxuan, QIU Yangyang, ZHANG Zhixuan, LI Baoying, LI Qiang, JIN Yongling, WANG Liyan. Effects of Kidney Bean Resistant and Susceptible Varieties on Growth and Development and Metabolic Enzymes of Aphis craccivora Koch.[J]. Journal of Henan Agricultural Sciences, 2026, 55(2): 98-105.

祝宇轩, 邱洋洋, 张志轩, 李宝莹, 李强, 金永玲, 王丽艳. 芸豆抗感品种对豆蚜生长发育及代谢酶的影响[J]. 河南农业科学, 2026, 55(2): 98-105.

Figures/Tables 6

References

［1］刘淑婷，王颖，王志辉，等. 不同品种芸豆淀粉及其抗性淀粉结构和物化特性比较［J］. 中国食品学报，2020，20（4）：49‑56.

LIU S T，WANG Y，WANG Z H，et al. Comparison of structure and physicochemical properties of kidney bean
starch and resistant starch from different varieties［J］.Journal of Chinese Institute of Food Science and Technology，2020，20（4）：49‑56.

［2］尹振功，孟宪欣，郭怡璠，等. 芸豆晕疫病抗性种质资源筛选及全基因组关联分析［J/OL］. 分子植物育种，2024：1‑9（2024‑05‑15）［2025‑03‑02］. https：//kns. cnki.net/kcms/detail/46. 1068. S. 20240514. 1113. 008.html.
YIN Z G，MENG X X，GUO Y F，et al. Screening of germplasm resources resistant to kidney bean halo blight and genome‑wide association study［J/OL］.Molecular Plant Breeding，2024：1‑9（2024‑05‑15）［2025‑03‑02］. https：//kns. cnki. net/kcms/detail/46. 1068. S. 20240514. 1113. 008. html.

［3］梁洪宇，张海燕，金永玲，等. 绿豆抗感虫品种对豆蚜种群生命参数的影响［J］. 中国植保导刊，2019，39（10）：5‑9.
LIANG H Y，ZHANG H Y，JIN Y L，et al. Effects of mung bean resistant and susceptible varieties on life parameters of Aphis craccivora Koch［J］.China Plant Protection，2019，39（10）：5‑9.

［4］康敬涛，高希武. 豆蚜对杀虫剂的敏感性及药剂对豆蚜羧酸酯酶和乙酰胆碱酯酶抑制作用的研究［J］.中国植保导刊，2009，29（9）：5‑7.
KANG J T，GAO X W. Study on the sensitivity of Aphis craccivora to insecticide and inhibiting effects of insecticide on Aphis craccivora CarE and AChE［J］.China Plant Protection，2009，29（9）：5‑7.

［5］梁洪宇，金永玲，张海燕，等. 豆蚜胁迫对绿豆抗、感品种营养物质和保护酶的影响［J］. 河南农业科学，2020，49（8）：72‑77.
LIANG H Y，JIN Y L，ZHANG H Y，et al. Effects of Aphis craccivora Koch infestation on the nutrient contents and protective enzyme activities in mung bean resistant and susceptible varieties［J］.Journal of Henan Agricultural Sciences，2020，49（8）：72‑77.

［6］孙峰，张如标，朱明，等. 不同药剂对小麦蚜虫的防治效果初探［J］. 上海农业科技，2024（6）：143‑144.
SUN F，ZHANG R B，ZHU M，et al. Preliminary study on the control effect of different chemicals on wheat aphids［J］.Shanghai Agricultural Science and Technology，2024（6）：143‑144.

［7］戴爱梅，丁志梅，张海军，等. 印楝素与脂肪酸甲酯联合使用对豆蚜的防治效果［J］. 环境昆虫学报，2022，44 （5）：1301‑1307.
DAI A M，DING Z M，ZHANG H J，et al. Evaluation of the control effect of azadirachtin and fatty acid methyl ester spray adjuvant on Aphis craccivora（Koch）［J］.Journal of Environmental Entomology，2022，44（5）：1301‑1307.

［8］刘锦，陈鹏，迟宝杰，等. 不同杀虫剂防治苜蓿蚜虫效果及对捕食性天敌影响［J］. 山东农业大学学报（自然科学版），2024，55（4）：587‑592.
LIU J，CHEN P，CHI B J，et al. Effect of different insecticides on alfalfa aphids and predatory natural enemies［J］.Journal of Shandong Agricultural University（Natural Science Edition），2024，55（4）：587‑592.
［9］刘晓蓓，张雨濛，FRANCIS F，等. 黄淮海麦区小麦品种苗期与成株期对蚜虫抗性综合鉴定［J］. 植物保护学报，2024，51（6）：1427‑1437.
LIU X B，ZHANG Y M，FRANCIS F，et al.Comprehensive aphid resistance evaluation of wheat varieties at seedling and adult stages in Huang‑Huai‑Hai region of China［J］. Journal of PlantProtection，2024，51（6）：1427‑1437.

［10］霍银亮，马银香，王新谱. 不同枸杞品种感蚜部位的物理性状及其抗蚜性［J］. 农业科学研究（中英文），2025，46（2）：54‑58.
HUO Y L，MA Y X，WANG X P. Study on the external traits of insect resistance of four kinds of wolfberry to cotton aphid［J］. Journal of Agricultural Sciences，2025，46（2）：54‑58.

［11］文婷婷，何玉梅，赵晓雁，等. 不同棉花品种对棉蚜的抗性评价研究［J］. 农业科技与信息，2024（6）：86‑89.
WEN T T，HE Y M，ZHAO X Y，et al. Evaluation of resistance of different cotton varieties to cotton aphid［J］. Agricultural Science‑Technology and Information，2024（6）：86‑89.

［12］史梦竹，傅建炜，李建宇，等. 运用年龄-龄期两性生命表模拟昆虫种群动态及其在害虫治理中的应用［J］. 昆虫学报，2023，66（2）：255‑266.
SHI M Z，FU J W，LI J Y，et al. Projection of insect population dynamics with age‑stage，two‑sex life table and its application in pest management［J］. Acta Entomologica Sinica，2023，66（2）：255‑266.

［13］吕军，唐桦，廖成家，等. 利用生命表评价天敌昆虫防治栗山天牛的效果［J］. 辽宁林业科技，2021（2）：6‑12.
LÜ J，TANG H，LIAO C J，et al. Evaluation of biocontrol effects against Massicus raddei by life table method［J］. Liaoning Forestry Science and Technology，2021（2）：6‑12.

［14］姜璠，邹航，徐震霆，等. 糖类对美国白蛾生长发育及消化酶活性的影响［J］. 福建农林大学学报（自然科学版），2025，54（3）：317‑324.
JIANG F，ZOU H，XU Z T，et al. Effect of carbohydrates feeding on development and digestive enzyme activity of Hyphantria cunea［J］. Journal of Fujian Agriculture and Forestry University（Natural Science Edition），2025，54（3）：317‑324.

［15］朱凯辉，尹仙枫，王芳，等. 寄主转换对棉蚜存活率与营养代谢的影响［J］. 植物保护学报，2024，51（2）：475‑483.
ZHU K H，YIN X F，WANG F，et al. Effect of host transformation on the survival rate and nutritional metabolism of cotton aphid，Aphis gossypii［J］. Journal of Plant Protection，2024，51（2）：475‑483.

［16］郝桂锋，刘迎，伍春玲，等. 二斑叶螨对阿维菌素不同抗性水平与解毒酶的关系分析［J］. 热带作物学报，2025，46（2）：389‑397.
HAO G F，LIU Y，WU C L，et al. Analysis of the relationship between abamectin resistance levels and detoxification enzymes of Tetranychus urticae［J］.Chinese Journal of Tropical Crops，2025，46（2）：389‑397.
［17］周昭旭，杨亚贤，钱秀娟，等. 取食2种寄主植物对番茄潜叶蛾生长发育及解毒酶活性的影响［J］. 甘肃农业大学学报，2024，59（4）：91‑96.
ZHOU Z X，YANG Y X，QIAN X J，et al. Effects of feeding two host plants on growth and development and detoxification enzyme activity of Tuta absoluta［J］.Journal of Gansu Agricultural University，2024，59（4）：91‑96.

［18］陈磊. 荞麦不同品种对西伯利亚龟象的抗性机理研究［D］. 呼和浩特：内蒙古农业大学，2022.
CHEN L.Study on resistance mechanism of different buckwheat varieties to Siberian turtle elephant［D］.Hohhot：Inner Mongolia Agricultural University，2022.

［19］沈海燕，梁运，陈正浩，等. 不同寄主植物对云南松毛虫生长发育、繁殖及生理酶活性的影响［J］.林业科学研究，2022，35（5）：63‑70.
SHEN H Y，LIANG Y，CHEN Z H，et al. Effects of different host plants on the growth，reproduction and physiological enzyme activity of Dendrolimus houi lajonquiere（Lepidoptera：Lasiocampidae）［J］. Forest Research，2022，35（5）：63‑70.

［20］王倩.飞蝗对四种不同植物的取食适应机制研究［D］. 泰安：山东农业大学，2023.
WANG Q. Study on feeding adaptation mechanism of migratory locust to four different plants［D］.Taian：Shandong Agricultural University，2023.

［21］张艳蕾. 草地贪夜蛾对玉米的选择性及不同玉米对其生长发育的影响［D］. 兰州：甘肃农业大学，2021.
ZHANG Y L. Selectivity of Spodoptera exigua to maize and the effects of different maize on its growth and development［D］. Lanzhou： Gansu Agricultural University，2021.

［22］DENG P，CHEN L J，ZHANG Z L，et al. Responses of detoxifying，antioxidant and digestive enzyme activities to host shift of Bemisia tabaci（Hemiptera：Aleyrodidae）［J］. Journal of Integrative Agriculture，2013，12（2）：296‑304.

［23］周琼，魏美才，欧晓明. 环氧苍耳素Ⅰ对菜青虫中肠消化酶和羧酸酯酶活性的影响［J］. 昆虫学报，2011，54（6）：729‑733.
ZHOU Q，WEI M C，OU X M. Effect of epoxyxanthatin Ⅰ from Xanthium sibiricum（Asteraceae）on the activity of midgut digestive enzymes and carboxylesterase in Pieris rapae（Lepidoptera：Pieridae）larvae［J］. Acta Entomologica Sinica，2011，54（6）：729‑733.

［24］吴国昭，胡林，叶茂，等. 大豆胰蛋白酶抑制剂和防御信号物质对斜纹夜蛾解毒酶的影响［J］.应用生态学报，2012，23（7）：1952‑1958.
WU G Z，HU L，YE M，et al. Effects of soybean trypsinase inhibitor and defense signaling compounds on detoxification enzymes in Spodoptera litura（F.）larvae［J］. Chinese Journal of Applied Ecology，2012，23（7）：1952‑1958.

［25］汤德良，王武刚. 棉花品种对棉铃虫体内酶活性的影响［J］. 棉花学报，1996，8（1）：36‑39.
TANG D L，WANG W G. Effects of cotton varieties on activities of enzymes in cotton bollworm［Helicoverpa armigera（hubner）］［J］. Cotton Science，1996，8（1）：36‑39.

［26］岳秋娟，姚淑敏，刘洋洋，等. 一个致倦库蚊杀虫剂敏感品系的筛选［J］. 昆虫学报，2013，56（4）：379‑384.
YUE Q J，YAO S M，LIU Y Y，et al. Screening of an insecticide‑susceptible strain of Culex pipiens quinquefasciatus（Diptera： Culicidae）［J］. Acta Entomologica Sinica，2013，56（4）：379‑384.

［27］朱诚棋，王忠，谢伟忠，等. 3种白木香叶片对黄野螟幼虫生长发育和保护酶活性的影响［J］. 中国植保导刊，2017，37（6）：19‑23.
ZHU C Q，WANG Z，XIE W Z，et al. Effects of Aquilaris sinensis leaves on development and protective enzymes activity of Heortia vitessoides Moore larval［J］. China Plant Protection，2017，37（6）：19‑23.

［28］赵士熙，吴中孚，吴刚，等. 水稻品种抗性对虫体酯酶的影响［J］. 植物保护学报，1993，20（3）：193‑198.
ZHAO S X，WU Z F，WU G，et al. The effects of antibiotic resistance of rice varieties on the esterase contents in brown planthopper［J］.Journal of Plant Protection，1993，20（3）：193‑198.

［29］SHEN L，LUO Q，SHEN Z P，et al.A new cytochalasin from endophytic Phomopsis sp.IFB‑E060［J］.Chinese Journal of Natural Medicines，2014，12（7）：512‑516.

［30］BEHRA M，COUSIN X，BERTRAND C，et al.Acetylcholinesterase is required for neuronal and muscular development in the zebrafish embryo［J］.Nature Neuroscience，2002，5（2）：111‑118.

[1]	CHAI Guanqun, ZHOU Lixing, QIN Song, FAN Chengwu, LIU Guihua, WANG Li, JIANG Ya. Screening of Kidney Bean Varieties for Safe Production in Cadmium Polluted Farmland of Northwest Guizhou [J]. Journal of Henan Agricultural Sciences, 2023, 52(6): 51-60.
[2]	LIANG Hongyu, JIN Yongling, ZHANG Haiyan, LIU Rui, SUN Yujia, GUO Simin, ZENG Yingting, WANG Liyan. Effects of Aphis craccivora Koch Infestation on the Nutrient Contents and Protective Enzyme Activities in Mung Bean Resistant and Susceptible Varieties [J]. Journal of Henan Agricultural Sciences, 2020, 49(8): 72-77.