Effects of Exogenous 6‐BA and GA3 on Ethylene Release Amount，Polyamine Content and Membrance Lipid Peroxidation of Melon Leaves

doi:10.15933/j.cnki.1004-3268.2026.01.012

Abstract

Abstract: The aims are to clarify the physiological mechanism of 6‐BA and GA₃regulating melon leaf senescence and to provide a theoretical basis for their application in delaying leaf senescence.Early‐maturing thick‐skinned variety‘Xuetong No.9’was used as the test materials，which were sprayed on the leaves by 30 mg/kg 6‐BA and 20 mg/kg GA₃ at flowering and fruit setting stage under protected cultivation conditions，with water as the control.The changes in relative chlorophyll content（SPAD value），ethylene release amount，polyamine content，catalase（CAT）activity，malondialdehyde（MDA）content，hydrogen peroxide（H_₂O_₂）content of leaves，and yield were investigated. The results showed that the SPAD values were increased by 1.78%—8.04% and 0.37%—6.08%，while ethylene release amount was reduced by 5.12%—28.46% and 2.34%—18.80% respectively with exogenous 6‐BA and GA₃treatments，and the contents of MDA，H₂O₂ and putrescine rescine in leaves were also decreased，while the contents of spermine，spermidine and CAT activity were increased，and the yields were also increased by 6.70% and 4.75% respectively.Overall，both exogenous hormone treatments showed more pronounced effects in the early stage，with their efficacy gradually diminishing over time.These findings indicate that 6‐BA and GA_₃ can enhance leaf anti‐aging capacity and delay senescence by regulating ethylene release，polyamine metabolism，and membrane lipid peroxidation.

Key words: Melon, 6‐BA, GA₃, Ethylene release, Polyamine, Membrance lipid peroxidation

摘要： 为明确6-苄基腺嘌呤（6-BA）和赤霉素（GA₃）调控甜瓜叶片衰老的生理机制并为其在延缓叶片衰老中的应用提供理论依据，以早熟厚皮甜瓜雪彤9号为试材，在设施栽培条件下于开花坐果期分别叶面喷施30 mg/kg 6-BA和20 mg/kg GA₃，以清水为对照，探讨叶片叶绿素相对含量（SPAD值）、乙烯释放量、多胺含量及过氧化氢酶（CAT）活性、丙二醛（MDA）含量、过氧化氢（H₂O₂）含量和产量的变化。结果表明，外源6-BA和GA₃处理后7~42 d，使甜瓜叶片SPAD值较对照提高1.78%~8.04%和0.37%~6.08%，乙烯释放量较对照降低5.12%~28.46%和2.34%~18.80%，同时叶片MDA、H₂O₂、腐胺（Put）含量也均有不同程度的降低，精胺（Spm）、亚精胺（Spd）含量和CAT活性有所升高，产量分别提高6.70%、4.75%；从整体上来看，2种外源激素处理前期效果较为明显，随时间的推移其作用效果逐渐减弱。综上所述，6-BA和GA₃可通过调控甜瓜叶片乙烯释放量、多胺含量和膜脂过氧化程度，提高叶片的抗衰老能力，延缓叶片衰老。

关键词: 甜瓜, 6-BA, GA3, 乙烯释放量, 多胺, 膜脂过氧化

CLC Number:

S652

ZHAO Weixing, LI Xiaohui, GAO Ningning, KANG Liyun, CHANG Gaozheng, LI Hailun, WANG Huiying. Effects of Exogenous 6‐BA and GA₃ on Ethylene Release Amount，Polyamine Content and Membrance Lipid Peroxidation of Melon Leaves[J]. Journal of Henan Agricultural Sciences, 2026, 55(1): 132-138.

赵卫星, 李晓慧, 高宁宁, 康利允, 常高正, 李海伦, 王慧颖. 外源6-BA 和GA3 对甜瓜叶片乙烯释放量、多胺含量及膜脂过氧化的影响[J]. 河南农业科学, 2026, 55(1): 132-138.

Figures/Tables 5

References

［1］刘文革，徐小利，潘秀清，等. 黄河故道地区西瓜甜瓜产业分析和建议［J］. 中国瓜菜，2022，35（8）：1‐11.
LIU W G，XU X L，PAN X Q，et al. Analysis and recommendation of commercial watermelon and melon production in the old course of the Yellow River［J］.China Cucurbits and Vegetables，2022，35（8）：1‐11.

［2］苗立祥，张豫超，杨肖芳，等. 厚皮甜瓜植株衰老进程相关生理指标变化初探［J］. 浙江农业学报，2011，23（5）：914‐919.

MIAO L X，ZHANG Y C，YANG X F，et al. Preliminary study on physiological indices related to natural senescence in muskmelon［J］. Acta Agriculturae Zhejiangensis，2011，23（5）：914‐919.

［3］吴冬云，朱碧岩，丁四兵，等. 6-BA和GA对水稻后期衰老的影响［J］.华南师范大学学报（自然科学版），2003，35（1）：119‐122.

WU D Y，ZHU B Y，DING S B，et al.The effect of 6‐BA and GA on senescence in the later stage of rice［J］.Journal of South China Normal University（Natural Science Edition），2003，35（1）：119‐122.

［4］张文学，彭春瑞，孙刚，等. 不同外源激素对二晚后期叶片衰老的影响［J］.江西农业学报，2007，19（2）：11‐13.

ZHANG W X，PENG C R，SUN G，et al. Effect of different external phytohormones on leaves senescence in late growth period of late‐season rice［J］.Acta Agriculturae Jiangxi，2007，19（2）：11‐13.

［5］李春喜，尚玉磊，姜丽娜，等. 不同植物生长调节剂对小麦衰老及产量构成的调节效应［J］.西北植物学报，2001，21（5）：931‐936.

LI C X，SHANG Y L，JIANG L N，et al. Regulation of plant growth regulator on leaves senescence and yield constitutions of wheat［J］.Acta Botanica Boreali‐Occidentalia Sinica，2001，21（5）：931‐936.

［6］张海娜，李存东，肖凯. 外源6-BA对棉花光合和叶片衰老特性的调控效应研究［J］.棉花学报，2007，19（6）：467‐471.

ZHANG H N，LI C D，XIAO K. Regulation effects of exogenous 6‐BA on photosynthesis and leaf senescence in cotton［J］. Cotton Science，2007，19（6）：467‐471.

［7］赵卫星，王文英，李晓慧，等. 外源6-BA和GA3对甜瓜叶片衰老过程中生理特性及产量影响［J］.中国农学通报，2016，32（31）：46‐50.

ZHAO W X，WANG W Y，LI X H，et al. Effects of exogenous 6‐BA and GA3 on physiological characteristics during leaf senescence and yield of melon ［J］. Chinese Agricultural Science Bulletin，2016，32（31）：46‐50.

［8］卢素萍，赵铭钦，刘鹏飞，等.光质对烤烟叶片衰老过程中内源激素含量和膜脂过氧化的影响［J］.中国农业科技导报，2016，18（6）：44‐51.

LU S P，ZHAO M Q，LIU P F，et al. Effect of light quality on endogenous hormone content and lipid peroxidation of membrane system during aging process of flue‐cured tobacco leaf［J］. Journal of Agricultural Science and Technology，2016，18（6）：44‐51.

［9］胡俊杰，张古文，胡齐赞，等. 低温胁迫对菜用大豆生长、叶片活性氧及多胺代谢的影响［J］.浙江农业学报，2011，23（6）：1113‐1118.

HU J J，ZHANG G W，HU Q Z，et al. Effects of chilling stress on growth，metabolism of reactive oxygen species and polyamines in vegetable soybean seedlings［J］.Acta Agriculturae Zhejiangensis，2011，23（6）：1113‐1118.

［10］刘建新，赵国林. 叶子花衰老过程中抗氧化酶、乙烯生成速率和多胺含量的变化［J］.园艺学报，2005，32（4）：722‐724.

LIU J X，ZHAO G L. Changes of antioxidant enzymes，ethylene production and polyamines in Bougainvillea glabra［J］.Acta Horticulturae Sinica，2005，32（4）：722‐724.

［11］刘俊，吉晓佳，刘友良. 检测植物组织中多胺含量的高效液相色谱法［J］.植物生理学通讯，2002，38（6）：596‐598.

LIU J，JI X J，LIU Y L. High performance liquid chromatography method for measuring polyamine content in plant tissue［J］.Plant Physiology Communications，2002，38（6）：596‐598.

［12］李合生.植物生理生化实验原理和技术［M］.北京：高等教育出版社，2000.

LI H S.Plant physiology biochemistry experiment principle and technology［M］.Beijing：Higher Education Press，2000.

［13］赵莉，潘远智，朱峤. GA₃、IBA和6-BA对香水百合开花特性及内源激素的影响［J］. 湖北农业科学，2012，51（8）：1385‐1389，1393.

ZHAO L，PAN Y Z，ZHU Q. Effects of 6‐BA，IBA and GA₃ on flowering and endogenous hormone content of Lilium casa Blanca［J］. Hubei Agricultural Sciences，2012，51（7）：1385‐1389，1393.

［14］李腾基，付志恵，陈鑫，等. 不同植物生长调节剂对墨兰成花生理和开花性状的影响［J］.分子植物育种，2024，22（17）：5777‐5786.

LI T J，FU Z H，CHEN X，et al. Effects of different plant growth regulators on flowering physiology and flowering characters of Cymbidium sinense［J］.Molecular Plant Breeding，2024，22（17）：5777‐5786.

［15］李文阳，马梦迪，郭红卫. 植物激素乙烯作用机制的最新进展［J］.中国科学：生命科学，2013，43（10）：854‐863.

LI W Y，MA M D，GUO H W. Advances in the action of plant hormone ethylene［J］.Scientia Sinica（Vitae），2013，43（10）：854‐863.

［16］FANG S，GAO K，HU W，et al. Foliar and seed application of plant growth regulators affects cotton yield by altering leaf physiology and floral bud carbohydrate accumulation［J］. Field Crops Research，2019，231：105‐114.

［17］任红旭，陈雄，王亚馥. 抗旱性不同的小麦幼苗在水分和盐胁迫下抗氧化酶和多胺的变化［J］. 植物生态学报，2001，25（6）：709‐715.

REN H X，CHEN X，WANG Y F. Changes in antioxidative enzymes and polyamines in wheat seedlings with different drought resistance under drought and salt stress［J］. Acta Phytoecologica Sinica，2001，25（6）：709‐715.

［18］曲亚英.几种外源激素防止甜椒早衰效应和生理机制研究［D］.兰州：甘肃农业大学，2005.

QU Y Y. Study on the effects and physiological mechanisms of several exogenous hormones in preventing premature senescence of sweet pepper［D］.Lanzhou：Gansu Agricultural University，2025.

［19］赵福庚，张国珍，张正钫，等. 花生叶片衰老中多胺代谢酶活性及游离多胺含量变化（简报）［J］.植物生理学通讯，1996，32（5）：351‐353.

ZHAO F G，ZHANG G Z，ZHANG Z F，et al.Changes of free polyamines levels and activities of some enzymes during senescence stage of peanut leaves （brief report）［J］. Plant Physiology Communications，1996，32（5）：351‐353.

［20］赵福庚，王晓云，王汉忠，等. 花生叶片生长发育过程中多胺代谢的变化［J］.作物学报，1999，25（2）：249‐253.

ZHAO F G，WANG X Y，WANG H Z，et al.The changes of polyamine metabolism in the process of growth and development of peanut leaves［J］.Acta Agronomica Sinica，1999，25（2）：249‐253.

［21］张青，叶茂炳，徐朗莱，等. 外源精胺、亚精胺作用下离体小麦叶片老化过程中蛋白水解酶活性和内源精胺、亚精胺含量的变化（简报）［J］. 植物生理学通讯，2000，36（6）：515‐516.

ZHANG Q，YE M B，XU L L，et al. Changes of proteolytic enzyme activity and endogenous spermine and spermidine content during aging of wheat leaves in vitro under the action of exogenous spermine and spermidine（brief report）［J］. Plant Physiology Communications，2000，36（6）：515‐516.

［22］张艳军，赵江哲，张可伟. 植物激素在叶片衰老中的作用机制研究进展［J］.植物生理学报，2014，50（9）：1305‐1309.

ZHANG Y J，ZHAO J Z，ZHANG K W. Research progress on mechanisms of phytohormones regulating leaf senescence［J］. Plant Physiology Journal，2014，50 （9）：1305‐1309.

［23］刘晓辉，张显，郑俊鶱，等. 激素预处理对低温胁迫下西瓜幼苗活性氧含量和抗氧化酶活性的影响［J］.西北植物学报，2014，34（4）：746‐752.

LIU X H，ZHANG X，ZHENG J X，et al. Effect of exogenous hormones on the content of active oxygen and activity of antioxidant enzymes of watermelon seedling under low temperature stress［J］.Acta Botanica Boreal‐Occidenttalia Sinica，2014，34（4）：746‐752.

［24］李金航，郭丽丽，孔祥生，等. 6-BA和GA₃对牡丹叶片衰老过程中生理特性的影响［J］. 植物生理学报，2014，50（8）：1243‐1247.

LI J H，GUO L L，KONG X S，et al. Effects of 6‐BA and GA₃ on physiological characteristics during leaf senescence of Paeonia suffruticosa［J］.Plant Physiology Journal，2014，50（8）：1243‐1247.

［25］LIAO Z G，ZHANG Y C，YU Q，et al. Coordination of growth and drought responses by GA‐ABA signaling in rice［J］.New Phytologist，2023，240（3）：1149‐1161.

［26］STOPARIĆ G，MAKSIMOVIĆ I.The effect of cytokinins on the concentration of hydroxyl radicals and the intensity of lipid peroxidation in nitrogen deficient wheat［J］.Cereal Research Communications，2008，36（4）：601‐609.

［27］王帅，王海波，王孝娣，等. 施硒和6-BA对葡萄叶片衰老与活性氧代谢的影响［J］.果树学报，2015，32（2）：206‐214.

WANG S，WANG H B，WANG X D，et al. Effects of selenium and 6‐BA on leaf senescence and active oxygen metabolism in grape［J］. Journal of Fruit Science，2015，32（2）：206‐214.

［28］郑莎莎，孙传范，孙红春，等.不同外源激素对花铃期棉花主茎叶生理特性的影响［J］. 中国农业科学，2009，42（12）：4383‐4389.

ZHENG S S，SUN C F，SUN H C，et al. Effects of different exogenous hormones on physiological characteristics of main stem leaves at flower and boll stage in cotton［J］. Scientia Agricultura Sinica，2009，42（12）：4383‐4389.

［29］杨卫兵，王振林，尹燕枰，等. 外源ABA和GA对小麦籽粒内源激素含量及其灌浆进程的影响［J］.中国农业科学，2011，44（13）：2673‐2682.

YANG W B，WANG Z L，YIN Y P，et al. Effects of spraying exogenous ABA or GA on the endogenous hormones concentration and filling of wheat grains［J］.Scientia Agricultura Sinica，2011，44（13）：2673‐2682.

［30］赵晓敏. 不同浓度GA3和6-BA处理对蝴蝶兰内源激素含量及开花品质的影响［D］. 银川：宁夏大学，2025.

ZHAO X M. Effects of different concentrarions of gibberellin and 6‐BA on endogenous hormone content and flowering quality of Phalaenopsis［D］.Yinchuan：Ningxia University，2025.

[1]	LI Hailun, GAO Ningning, GUO Yaomiao, SUN Dongling, WANG Yueling, XIE Kuixi, LI Pengfei, DUAN Shixiang, ZHAO Weixing, YANG Sen. Genome‑wide Identification and Expression Analysis of TCP Transcription Factor Family in Melon [J]. Journal of Henan Agricultural Sciences, 2025, 54(9): 127-140.
[2]	PAN Juxiu, SHEN Qi, WANG Xiaoting, KANG Qi, WUJISIGULENG, HE Weizhong, WANG Cheng. Analysis of Soil Heavy Metal Pollution Levels and Sources in Melon Fields in Two Typical Producing Areas of Xinjiang [J]. Journal of Henan Agricultural Sciences, 2025, 54(2): 88-98.
[3]	TANG Bei, LI Yuanyuan, YANG Qiaoyun, HONG Zehua. Effects of Nitrogen Reduction Combined with Spent Mushroom Substrates on the Yield of Oriental Melon and Soil Environment under Different Soil Moisture Levels [J]. Journal of Henan Agricultural Sciences, 2025, 54(11): 123-133.
[4]	LIU Xue, WANG Xina, LI Xuefang, HAO Wenyue, HUO Qingzhu, TAN Junli. Effects of Chloride Varieties and Concentration on Watermelon Growth and Root Vitality [J]. Journal of Henan Agricultural Sciences, 2023, 52(3): 118-126.
[5]	LI Xiangchun, SUI Yihu, TANG Jingwen, HU Nengbing. Effects of the Combination of Four Cultivation Factors on Agronomic，Fruit Traits and Yield of the Intercropping Systerm of Watermelon‐Pepper in Greenhouses [J]. Journal of Henan Agricultural Sciences, 2023, 52(12): 116-126.
[6]	JIA Licong, WANG Lianjun, YANG Zitong, SHANG Lili, ZHANG Lei, QIU Pengfei, WANG Cuijuan, HAN Junjie, XIN Guosheng. Effect of Combinations with Different Concentrations of IAA and 6‐BA on Shoot Tip Culture and Phenotypic Variation of Sweetpotato [J]. Journal of Henan Agricultural Sciences, 2023, 52(12): 42-48.
[7]	XIE Yutian, ZHANG Xinye, SU Yanping, WU Shuang, ZHANG Zeyin, CHU Zhuodong, FENG Xue, SUN Yanxiang. Identification and Expression Analysis of PAO Gene Family in Kiwifruit [J]. Journal of Henan Agricultural Sciences, 2023, 52(10): 109-119.
[8]	YANG Bo, DUAN Minglei, YANG Tong. Research on the Classification Method of Watermelon Seed Vigor Level Based on Hyperspectral Imaging Technology [J]. Journal of Henan Agricultural Sciences, 2022, 51(9): 151-158.
[9]	KANG Liyun, LI Xiaohui, GAO Ningning, LIANG Shen, LI Hailun, WANG Huiying, CHANG Gaozheng, XU Xiaoli, ZHAO Weixing. Analysis of Yield，Quality and Potassium Absorption and Utilization of Different Genotypes of Melon under Low Potassium Condition [J]. Journal of Henan Agricultural Sciences, 2022, 51(8): 99-106.
[10]	LIU Chenggong, LI He, HAN Zichao, SU Tingting, ZHANG Yueyang, ZHANG Yao, LI Zhongqing, WANG Aiying , ZHU Jianbo. Identification and Characterization of SnRK2 Gene Family in Melon [J]. Journal of Henan Agricultural Sciences, 2022, 51(5): 108-116.
[11]	QIN Tao. Effects of Pepper Straw Returning Amount on Rhizosphere Soil Microenvironment and Quality of Watermelon [J]. Journal of Henan Agricultural Sciences, 2021, 50(9): 79-86.
[12]	WANG Baoping, ZHOU Jing, SHI Xiangyuan, LI Xinxin, WANG Xiuhong, DU Huiping. Effects of Different Agricultural Waste Compound Substrates on Photosynthetic Characteristics,Yield and Quality of Watermelon [J]. Journal of Henan Agricultural Sciences, 2021, 50(6): 116-124.
[13]	ZHAO Weixing, KANG Liyun, GAO Ningning, LIANG Shen, CHANG Gaozheng, XU Xiaoli, LI Hailun, WANG Huiying, LI Xiaohui. Relationship between Variation of Leaf Defensive Enzyme Activities and Resistance of Watermelon Cultivar to Zucchin Yellow Mosaic Virus [J]. Journal of Henan Agricultural Sciences, 2021, 50(5): 92-98.
[14]	KANG Liyun, CHANG Gaozheng, MA Zhenghua, LI Xiaohui, GAO Ningning, LIANG Shen, XU Xiaoli, LI Hailun, WANG Huiying, ZHAO Weixing. Preliminary Screening of Low Potassium Tolerant Genotypes in Muskmelon Seedling Stage [J]. Journal of Henan Agricultural Sciences, 2020, 49(3): 113-120.
[15]	SHU Xiuyu. Effects of CO2 Enrichment on Growth,Physiological and Biochemical Characteristics of Watermelon Seedlings under Salt Stress [J]. Journal of Henan Agricultural Sciences, 2020, 49(12): 107-114.

Effects of Exogenous 6‐BA and GA₃ on Ethylene Release Amount，Polyamine Content and Membrance Lipid Peroxidation of Melon Leaves

外源6-BA 和GA3 对甜瓜叶片乙烯释放量、多胺含量及膜脂过氧化的影响

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 5

References

Related Articles 15

Recommended Articles

Metrics