Abstract: Using Australian four season large leaf coriander seeds as experimental materials，different mass fractions of colchicine（0.1%，0.2%，0.4%，0.6%，0.8%）and different time periods（2，4，6，8，10 h）were used for mutagenesis treatment to study the effect of colchicine on the induction of tetraploid coriander.The results showed that with the increase of colchicine concentration and the prolongation of treatment time，the germination rate and seedling rate of coriander seeds showed a significant downward trend.Among them，0.4% colchicine treatment for 2 h was the optimal induction condition，with the highest germination rate，seedling rate，and induction rate of 80%，60%，and 18.7%，respectively.The physiological indicators of coriander seedlings significantly improved，with chlorophyll a+b，soluble protein，and soluble sugar contents of 1.49，9.87，and 17.54 mg/g，respectively，which increased by 88.61%，58.97%，and 53.88% compared to the control.Through cytological identification，it was found that compared with diploid plants，tetraploid plants showed a significant reduction in the number of stomata per unit area of their leaves，an increase in stomata，and a doubling of the chromosome number in root tip cells（4n=44）.In addition，tetraploid coriander plants also exhibited excellent traits such as wide and thick green leaves，thicker and longer purple stems，strong aroma，and larger flowers and

Key words: Coriander, Colchicine, Chromosome doubling, Tetraploid induction

摘要： 以澳洲四季大叶香菜种子为试验材料，采用不同质量分数的秋水仙素（0.1%、0.2%、0.4%、0.6%、0.8%）及不同时间（2、4、6、8、10 h）进行诱变处理，研究秋水仙素对香菜四倍体诱导的影响。结果显示，随着秋水仙素质量分数的增加和处理时间的延长，香菜种子的发芽率和成苗率呈显著的下降趋势。其中，0.4%秋水仙素处理2 h为最佳诱导条件，发芽率、成苗率和诱导率最高，分别为80%、60%和18.7%。香菜幼苗生理指标显著提高，叶绿素a+b、可溶性蛋白和可溶性糖含量分别为1.49、9.87、17.54 mg/g，较蒸馏水对照提高88.61%、58.97%和53.88%。经细胞学鉴定发现，与二倍体植株相比，四倍体植株的叶片单位面积内气孔数目明显减少，气孔增大，根尖细胞染色体数目加倍（4n=44）。此外，香菜四倍体植株还表现出叶宽大浓绿、茎增粗长高呈紫色、气味浓郁、花及花序变大等优良性状。

关键词: 香菜, 秋水仙素, 染色体加倍, 四倍体诱导