Abstract: In order to investigate the effects of electron beam irradiation on degradation of 21 prohibited pesticide residues and active components of bupleurum，the content changes of 21 prohibited pesticides in bupleurum were analyzed by gas chromatography‑mass spectrometry（GC‑MS/MS），and the content changes of active components in bupleurum were determined by high performance liquid chromatography（HPLC）after electron beam treatment of 4，6，8，10 kGy.The results showed that the degradation rates of 21 prohibited pesticides in bupleurum increased with the increase of irradiation dose，and the maximal degradation rates ranged from 25.58% to 56.61%. The degradation rates of methyl parathion，fipronil sulfone，fipronil and monocrotophos in bupleurum after the 10 kGy irradiation treatment were significantly higher than those of 8 kGy，while the degradation rates of the other 17 pesticides did not show significant difference between 8 kGy and 10 kGy irradiation treatments. In addition，there was no significant change in the contents of saikosaponin a and d after different doses of irradiation treatments.Therefore，taking costs into account，it is concluded that 8 kGy electron beam irradiation can effectively degrade 21 prohibited pesticide residues in bupleurum，and has no significant effect on the content of active components in bupleurum，which can provide technical support for the degradation of 21 pesticides in bupleurum by irradiation.

Key words: Electron beam irradiation, Bupleurum, Pesticide degradation, Saikosaponin, GC?MS/MS, HPLC

摘要： 为探明电子束辐照对柴胡中21种禁用农药残留降解及柴胡有效成分的影响，采用4、6、8、10 kGy电子束处理含有21种禁用农药残留的柴胡样品，利用气相色谱-质谱联用（GC-MS/MS）技术分析不同辐照剂量处理后柴胡中21种禁用农药含量的变化，并利用高效液相色谱法（HPLC）测定柴胡有效成分含量的变化。结果表明，随辐照剂量的增加，柴胡中21种禁用农药的降解率升高，最高降解率在25.58%~56.61%。其中10 kGy辐照处理柴胡中甲基对硫磷、氟虫腈砜、氟虫腈、久效磷的降解率显著高于8 kGy辐照处理，而其他17种农药的降解率在8 kGy与10 kGy辐照处理间差异不显著。此外，不同剂量辐照处理后柴胡中柴胡皂苷a、d含量无明显变化。综合考虑成本因素认为，8 kGy电子束辐照能有效降解柴胡中21种禁用农药残留，对柴胡中有效成分含量无明显影响，可为辐照降解柴胡中的21种农药提供技术支持。

关键词: 电子束辐照, 柴胡, 农药降解, 柴胡皂苷, 气相色谱-质谱联用, 高效液相色谱法