Abstract: EIL(ethylene‑insensitive 3‑like)genes play important roles in the transduction involved in the ethylene signaling pathway and development of plants.In order to understand the information of EIL gene family members in Capsicum annuum L.,this study analyzed the physicochemical properties,protein structure，phylogeny,gene architecture,conserved domains,promoter cis‑acting elements,the expression patterns of CaEILs genes in different tissue parts and under different abiotic stress conditions using bioinformatics.The expression pattern of CaEILs in pepper leaves under saline‑alkali stress was also investigated using real‑time fluorescence quantitative PCR(qRT‑PCR).The results showed that nine CaEILs of Capsicum annuum L.were distributed on six chromosomes.The number of amino acids,molecular weight and aliphatic index were from 209 to 677,23.77 to 76.07 ku，and 63.10 to 87.75,respectively and they were mainly acid,hydrophilic,and unstable nuclear proteins. Phylogenetic analysis showed that CaEILs were divided into four subfamily groups,nine CaEILs were expressed in different degrees in roots,stems,leaves,buds,and flowers,and low temperature stress,high temperature stress,high salt stress,and drought stress induced the expression of CaEILs,which responded to the above abiotic stresses to different degrees. In addition，qRT‑PCR was performed to detect CaEILs expression in pepper leaves under saline‑alkali stress，and it was found that with the extension of treatment time，the expression of CaEIL1,CaEIL2,CaEIL4,CaEIL5,and CaEIL8 showed an increasing trend，while the expression of CaEIL3,CaEIL6,CaEIL7,and CaEIL9 showed an decreasing trend.The above results lay a foundation for the study of the functions of CaEILs and their mechanisms of action in response to saline‑alkali stress.

Key words: Capsicum annuum L. , EIC gene family, Gene structure, Saline?alkali stress, Expression

摘要： EIL（Ethylene‑insensitive 3‑like）基因在参与植物乙烯信号通路的转导和生长发育过程中发挥着重要作用。为了解辣椒EIL基因家族成员信息，利用生物信息学手段对辣椒EIL基因家族成员的理化性质、蛋白质结构、系统进化、基因结构、保守域、启动子顺式作用元件及CaEILs基因在不同组织部位和不同非生物胁迫条件下的表达模式进行分析。同时使用实时荧光定量PCR（qRT-PCR）对辣椒叶片中CaEILs在盐碱胁迫下的表达模式进行研究。结果表明，辣椒9个CaEILs分布在6条染色体上，氨基酸数目、蛋白质理论分子质量和脂肪系数分别介于209~677、23.77~76.07 ku和63.10~87.75，且主要为酸性、亲水的不稳定性核蛋白。系统进化分析显示，CaEILs被分成了4个亚家组，9个CaEILs在根、茎、叶、花蕾、花中具有不同程度的表达，而低温、高温、高盐、干旱胁迫下均能诱导CaEILs的表达，其对以上非生物胁迫均具有不同程度的响应。此外，利用qRT-PCR对盐碱胁迫下辣椒叶片中CaEILs 进行了检测，结果发现，随着处理时间的延长，CaEIL1、CaEIL2、CaEIL4、CaEIL5、CaEIL8的表达量整体呈上升趋势，而CaEIL3、CaEIL6、CaEIL7、CaEIL9的表达量整体呈下降趋势。

关键词: 辣椒, EIL基因家族, 基因结构, 盐碱胁迫, 表达