Journal of Henan Agricultural Sciences ›› 2022, Vol. 51 ›› Issue (3): 146-153.DOI: 10.15933/j.cnki.1004-3268.2022.03.017

• Animal Science Veterinary Medicine • Previous Articles     Next Articles

Molecular Epidemiological Investigation and Genetic Evolution Analysis of ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus in Henan Province in 2020

GUO Zhanda1,DU Jimei1,GUO Ziyi2,WANG Xingang3,ZHANG Yanhua4   

  1. (1.Henan Dahenong Animal Husbandry Co.Ltd. ,Zhengzhou 450000,China;2.College of Veterinary Medicine,Henan
    Agricultural University,Zhengzhou 450000,China;3.Shangqiu Meilan Bio⁃Engineering Co.Ltd. ,Zhecheng 476200,China;4.College of Animal Science and Technology,Henan Agricultural University,Zhengzhou 450000,China)
  • Received:2021-07-30 Published:2022-03-15 Online:2022-05-24

2020 年河南省猪繁殖与呼吸综合征病毒的分子流行病学调查及ORF5 基因变异分析

郭占达1,杜季梅1,郭子仪2,王新港3,张彦华4   

  1. (1.河南省大河农畜牧科技有限公司,河南 郑州 450000;2.河南农业大学动物医学院,河南 郑州 450000;3.商丘美兰生物工程有限公司,河南 柘城 476200;4.河南农业大学动物科学院,河南 郑州 450000)
  • 通讯作者: 张彦华(1989-),女,河南郑州人,讲师,主要从事动物遗传育种研究。E-mail:YHZhang@henau.edu.cn
  • 作者简介:郭占达(1989-),男,河南濮阳人,硕士,主要从事分子病原学研究。E-mail:401005049@qq.com
  • 基金资助:
    国家自然科学基金项目(31772781)

Abstract: In order to monitor the prevalence and variation of porcine reproductive and respiratory syndrome virus(PRRSV)in Henan Province,a total of 401 clinical samples of porcine reproductive and respiratory syndrome were collected in Henan Province in 2020 and analyzed by TaqMan real⁃time quantitative PCR(FQ⁃PCR).The results showed that a total of 100 positive samples were detected,with a positive rate of 24.9%. The ORF5 genes of 18 PRRSV epidemic strains were sequenced for homologous alignment and genetic evolution analysis. Nucleotide homology analysis showed that the ORF5 gene nucleotide homology among 18 strains was above 83.4%,and the nucleotide homology with the reference strain was 82.9%—99.7%.The results of genetic evolution analysis showed that all the 18 PRRSV strains belonged to American genotype,including lineage 1,lineage 5 and lineage 8.Two PRRSV strains belonged to lineage 8 represented by JXAl,one PRRSV strain belonged to lineage 5 represented by VR2332 and 15 PRRSV strains belonged to lineage 1 represented by NADC30. The results of amino acid sequence analysis showed that the 13th amino acid virulence related site of HN⁃TH1⁃2020,HY⁃PY1⁃2020,HN⁃XY1⁃2020 and the 151st amino acid virulence related site of HN⁃XC1⁃2020 had different degrees of mutation.

Key words: Henan Province, Porcine reproductive and respiratory syndrome virus, Epidemiology, ORF5 gene, Variation

摘要: 为了解河南省猪繁殖与呼吸综合征病毒(PRRSV)的流行及变异情况,对2020年采集于河南的401份PRRSV感染疑似病料进行TaqMan荧光定量PCR(FQ-PCR)检测。结果显示,共检测出100份阳性样品,阳性率为24.9%。对其中18株PRRSV流行毒株ORF5基因进行测序、同源性比对和遗传进化分析。同源性比对结果显示,18株PRRSV流行毒株ORF5基因核苷酸序列同源性在83.4%以上,与参考毒株同源性为82.9%~99.7%。遗传进化分析结果显示,18株PRRSV流行毒株序列均为美洲株,分属于1、5、8三个谱系,其中2株属于以JXAl为代表的谱系8,1株属于以VR2332为代表的谱系5,15株属于以NADC30为代表的谱系1。氨基酸比对结果显示,HN-TH1-2020、HY-PY1-2020、HN-XY1-2020毒株的13位以及HN-XC1-2020毒株的151位氨基酸毒力相关位点发生了不同程度的突变。

关键词: 河南省, 猪繁殖与呼吸综合征病毒, 分子流行病学, ORF5基因, 变异

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