Expression Characteristics of AaHsp90 in Antheraea assama under Different Temperature and Starvation Stress

doi:10.15933/j.cnki.1004-3268.2022.02.016

Journal of Henan Agricultural Sciences ›› 2022, Vol. 51 ›› Issue (2): 133-138.DOI: 10.15933/j.cnki.1004-3268.2022.02.016

• Animal Science Veterinary Medicine • Previous Articles Next Articles

Expression Characteristics of AaHsp90 in Antheraea assama under Different Temperature and Starvation Stress

YANG Weike¹，LIU Zenghu¹，TANG Fenfen²，HU Changxiong¹，DONG Zhanpeng¹

（1.Sericulture and Apiculture Research Institute，Yunnan Academy of Agricultural Sciences，Mengzi 661101，China；2.College of Biological and Agricultural Sciences，Honghe University，Mengzi 661101，China）

Received:2021-08-19 Published:2022-02-15 Online:2022-04-18

不同温度和饥饿胁迫下琥珀蚕AaHsp90 基因的表达特征

杨伟克¹，刘增虎¹，唐芬芬²，胡昌雄¹，董占鹏¹

（1.云南省农业科学院蚕桑蜜蜂研究所，云南蒙自 661101；2.红河学院生物科学与农学学院，云南蒙自 661101）

通讯作者: 董占鹏（1970-），男，云南江川人，研究员，博士，主要从事家蚕种质资源收集、改良利用和品种选育。E-mail：scsdong@163.com
作者简介:杨伟克（1985-），男，河南许昌人，助理研究员，硕士，主要从事蚕资源生物学研究。E-mail：WKSUN1985@163.com
基金资助:
国家自然科学基金项目（31360586）；云南省基础研究计划面上项目（2022FB226）；云南省现代农业蚕桑产业技术体系建设
项目（2019KJTX006）；红河学院博士专项（BS2003）；云南省科技计划重点研发专项（202102AE090010）

Abstract

Abstract: In order to investigate the influences of different temperature and starvation stress on the expression of AaHsp90 and reveal the molecular mechanism of adaptation to environment in Antheraea assama，with the normal feeding group at 26 ℃ as the control，the expression change of AaHsp90 was detected by real‑time PCR in midgut，fat body and hemlymph after high temperature stress at 38℃，low temperature stress at 4℃ and starvation stress respectively for different time on the third day of the fifth larvae.Results showed that the expressions of AaHsp90 in midgut，fat body and hemlymph of Antheraea assama were increased obviously at first and then decreased sharply with the prolongation of treatment time at 38℃.There has a certain inhibitory effect on the expression of AaHsp90 in midgut，fat body and hemolymph after tretment with 4℃ for different time. After treatment with starvation，the AaHsp90 expressions were increased at 12 h and 18 h and decreased sharply at 24 h in midgut，fat body and hemolymph of Antheraea assama.Comprehensive analysis showed that high temperature and starvation stress could induce the expression of AaHsp90，while low temperature stress mainly suppressed its expression. The AaHsp90 protein in Antheraea assama may play an important role in the process of adaptation to high temperature and starvation stress.

Key words: Heat shock protein 90, Antheraea assama, Temperature stress, Starvation stress, Expressing characteristics

摘要： 为了探究高温、低温和饥饿胁迫对琥珀蚕热激蛋白AaHsp90 基因表达水平的影响，揭示琥珀蚕适应环境的分子机制，对5龄第3天琥珀蚕幼虫分别进行38℃高温、4℃低温和饥饿胁迫处理，以26 ℃正常饲养组为对照，采用实时荧光定量PCR检测分析中肠、脂肪体和血淋巴AaHsp90 基因的相对表达量变化情况。结果表明，在测定的时间范围内，38℃高温处理后，中肠、脂肪体和血淋AaHsp90 基因的表达量均呈现先明显增加，后随着处理时间的延长急剧减弱的趋势。4℃低温处理对琥珀蚕幼虫中肠、脂肪体和血淋巴Aahsp90 基因的表达有一定的抑制作用。饥饿处理组中，琥珀蚕血淋巴、脂肪体和中肠AaHsp90 基因在饥饿12 h和18 h呈现上调表达趋势，而在饥饿24 h时，AaHsp90 基因的表达量急剧降低。综上可知，高温和饥饿胁迫能诱导琥珀蚕Aahsp90 基因的表达，而低温胁迫主要抑制AaHsp90 基因的表达，提示AaHsp90蛋白在琥珀蚕幼虫适应高温和饥饿胁迫的过程中发挥了重要作用。

关键词: 琥珀蚕, 热激蛋白90, 温度胁迫, 饥饿胁迫, 表达特征

CLC Number:

S885.9
Q966

YANG Weike, LIU Zenghu, TANG Fenfen, HU Changxiong, DONG Zhanpeng. Expression Characteristics of AaHsp90 in Antheraea assama under Different Temperature and Starvation Stress[J]. Journal of Henan Agricultural Sciences, 2022, 51(2): 133-138.

杨伟克, 刘增虎, 唐芬芬, 胡昌雄, 董占鹏. 不同温度和饥饿胁迫下琥珀蚕AaHsp90 基因的表达特征[J]. 河南农业科学, 2022, 51(2): 133-138.

Figures/Tables 5

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Expression Characteristics of AaHsp90 in Antheraea assama under Different Temperature and Starvation Stress

不同温度和饥饿胁迫下琥珀蚕AaHsp90 基因的表达特征

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