阳光玫瑰葡萄VvABF基因家族鉴定与采后ClO2处理的表达分析

doi:10.15933/j.cnki.1004-3268.2026.06.012

河南农业科学 ›› 2026, Vol. 55 ›› Issue (6): 120-131.DOI: 10.15933/j.cnki.1004-3268.2026.06.012

阳光玫瑰葡萄VvABF基因家族鉴定与采后ClO₂处理的表达分析

袁一林^1,2，岳红³，王良睿⁴，李宇轩⁴，郑璞帆¹，陈婷⁵，苗云刚⁶，李晓雪¹，谭丽平⁷

（1.天津市农业科学院农产品保鲜与加工技术研究所［国家农产品保鲜工程技术研究中心（天津）］/蔬菜生物育种国家重点实验室/农业农村部农产品贮藏保鲜重点实验室/天津市农产品采后生理与贮藏保鲜重点实验室，天津 300384；2.天津大学药物科学与技术学院，天津300072；3. 天津科技大学食品科学与工程学院，天津300457；4.天津大学生命科学学院，天津 300072；5.福建省农业科学院果树研究所，福建福州 350013；6.山东华旭包装制品有限公司，山东淄博 255130；7.北京金田麦国际食品有限公司，北京 101407）

收稿日期:2025-12-20 接受日期:2026-02-12 出版日期:2026-06-15 发布日期:2026-06-18
通讯作者: 李晓雪，助理研究员，博士，主要从事果蔬采后分子生物学机制研究。E-mail：m15822139395@163.com 谭丽平，高级工程师，硕士，主要从事农产品加工及贮藏工程研究。E-mail：tlp0913@126.com
作者简介:袁一林，在读本科生，研究方向：生物信息学。E-mail：739283740@qq.com
基金资助:
国家级十四五重点专项（2024YFD2100104）；天津市自然科学基金青年项目（24JCQNJC01120）；天津市科技帮扶项目（23ZYCGSN00920，24ZYCGSN00940）；福建省科技计划项目（2023R1086）

Identification of VvABF Gene Family and Expression Analysis under Post⁃harvest ClO₂Treatment in Shine⁃Muscat Grape

YUAN Yilin^1,2，YUE Hong³，WANG Liangrui⁴，LI Yuxuan⁴，ZHENG Pufan¹，CHEN Ting⁵，MIAO Yungang⁶，LI Xiaoxue¹，TAN Liping⁷

（1.Institute of Agricultural Product Preservation and Processing Technology，Tianjin Academy of Agricultural Sciences［National Agricultural Product Fresh⁃Keeping Engineering and Technology Research Center（Tianjin）］/National Key Laboratory of Vegetable Biological Breeding/Key Laboratory of Agricultural Product Storage and Preservation，Ministry of Agriculture and Rural Affairs/Tianjin Key Laboratory of Postharvest Physiology and Storage and Preservation of Agricultural Products，Tianjin 300384，China；2.School of Pharmaceutical Science and Technology，Tianjin University，Tianjin 300072，China；3.School of Food Science and Engineering，Tianjin University of Science and Technology，Tianjin 300457，China；4.School of Life Sciences，Tianjin University，Tianjin 300072，China；5.Fruit Research Institute，Fujian Academy of Agricultural Sciences，Fuzhou 350013，China；6.Shandong Sinocoroplast Packing Co.，Ltd.，Zibo 255130，China；7.Beijing Jintianmai International Food Co.，Ltd. ，Beijing 101407，China）

Received:2025-12-20 Accepted:2026-02-12 Published:2026-06-15 Online:2026-06-18

摘要/Abstract

摘要： ABA响应元件结合因子（ABRE binding factor，ABF）是bZIP转录因子的一个重要亚家族，在脱落酸（Abscisic acid，ABA）依赖型和非依赖型信号通路中发挥关键作用。通过生物信息学方法，从阳光玫瑰葡萄转录组测序数据中鉴定VvABF家族成员，对其进行生物信息学分析，并结合qRT-PCR分析其在不同贮藏天数和不同二氧化氯（ClO₂）浓度处理下的表达模式。结果表明，阳光玫瑰葡萄VvABF家族共有13个成员，可分为3个亚家族，每个成员含有3~6个外显子，在10条染色体上均有分布，共4对基因存在共线性关系。VvABF家族蛋白均为亲水蛋白且均不稳定，整个VvABF家族共有7个保守基序和6个保守结构域超家族，可能与非特异性丝氨酸/苏氨酸蛋白激酶如D7TPP4_VITVI等相互作用。此外，8 g/kgClO₂处理下VvABF2、VvABF4、VvABF9和VvABF13表达量显著提高。ClO₂可能通过提高VvABF2、VvABF4、VvABF9和VvABF13基因表达量来减轻采后阳光玫瑰葡萄果实褐变与衰老。

关键词: 阳光玫瑰葡萄, ABF转录因子, 采后贮藏品质, ClO₂保鲜处理, 转录组测序, 生物信息学分析

Abstract: Abscisic acid（ABA）⁃responsive element（ABRE）binding factor proteins（ABF）constitute a crucial subfamily of bZIP transcription factors that play pivotal roles in both ABA⁃dependent and ABA⁃independent signaling pathways.Through bioinformatics approaches，members of the VvABF family were identified from transcriptome sequencing data of Shine⁃Muscat grape，followed by bioinformatics analysis.Their expression patterns under different storage days and varying chlorine dioxide（ClO₂）concentration treatments were further investigated using qRT⁃PCR analysis.The results showed that the VvABF family comprised thirteen members dividing into three subfamilies and each member contained three to six exons，and the VvABF family members distributed across ten chromosomes with four gene pairs exhibiting collinear relationships.The VvABF family proteins were all hydrophilic and unstable.The entire VvABF family had seven conserved motifs and six conserved structural domains superfamily，which might interact with non⁃specific serine/threonine protein kinases such as D7TPP4_VITVI. Notably，8 g/kg ClO₂treatment significantly increased the expression levels of VvABF2，VvABF4，VvABF9，and VvABF13.ClO₂may mitigate post⁃harvest browning and aging of Shine⁃Muscat grape fruits by increasing the expression levels of VvABF2，VvABF4，VvABF9，and VvABF13genes.

Key words: Shine?Muscat grape, ABF transcription factor, Post?harvest storage quality, ClO₂preservation treatment, Transcriptome sequencing, Bioinformatics analysis

中图分类号:

S663.1

袁一林, 岳红, 王良睿, 李宇轩, 郑璞帆, 陈婷, 苗云刚, 李晓雪, 谭丽平. 阳光玫瑰葡萄VvABF基因家族鉴定与采后ClO₂处理的表达分析[J]. 河南农业科学, 2026, 55(6): 120-131.

YUAN Yilin, YUE Hong, WANG Liangrui, LI Yuxuan, ZHENG Pufan, CHEN Ting, MIAO Yungang, LI Xiaoxue, TAN Liping. Identification of VvABF Gene Family and Expression Analysis under Post⁃harvest ClO₂Treatment in Shine⁃Muscat Grape[J]. Journal of Henan Agricultural Sciences, 2026, 55(6): 120-131.

图/表 11

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阳光玫瑰葡萄VvABF基因家族鉴定与采后ClO₂处理的表达分析

Identification of VvABF Gene Family and Expression Analysis under Post⁃harvest ClO₂Treatment in Shine⁃Muscat Grape

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