河南农业科学 ›› 2026, Vol. 55 ›› Issue (6): 120-131.DOI: 10.15933/j.cnki.1004-3268.2026.06.012

• 园艺 • 上一篇    下一篇

阳光玫瑰葡萄VvABF基因家族鉴定与采后ClO2处理的表达分析

袁一林1,2,岳红3,王良睿4,李宇轩4,郑璞帆1,陈婷5,苗云刚6,李晓雪1,谭丽平7   

  1. (1.天津市农业科学院 农产品保鲜与加工技术研究所[国家农产品保鲜工程技术研究中心(天津)]/蔬菜生物育种国家重点实验室/农业农村部农产品贮藏保鲜重点实验室/天津市农产品采后生理与贮藏保鲜重点实验室,天津 300384;2.天津大学药物科学与技术学院,天津300072;3. 天津科技大学食品科学与工程学院,天津300457;4.天津大学 生命科学学院,天津 300072;5.福建省农业科学院 果树研究所,福建 福州 350013;6.山东华旭包装 制品有限公司,山东 淄博 255130;7.北京金田麦国际食品有限公司,北京 101407)
  • 收稿日期:2025-12-20 接受日期:2026-02-12 出版日期:2026-06-15 发布日期:2026-06-18
  • 通讯作者: 李晓雪,助理研究员,博士,主要从事果蔬采后分子生物学机制研究。E-mail:m15822139395@163.com 谭丽平,高级工程师,硕士,主要从事农产品加工及贮藏工程研究。E-mail:tlp0913@126.com
  • 作者简介:袁一林,在读本科生,研究方向:生物信息学。E-mail:739283740@qq.com
  • 基金资助:
    国家级十四五重点专项(2024YFD2100104);天津市自然科学基金青年项目(24JCQNJC01120);天津市科技帮扶项目(23ZYCGSN00920,24ZYCGSN00940);福建省科技计划项目(2023R1086)

Identification of VvABF Gene Family and Expression Analysis under Post⁃harvest ClO2Treatment in Shine⁃Muscat Grape

YUAN Yilin1,2,YUE Hong3,WANG Liangrui4,LI Yuxuan4,ZHENG Pufan1,CHEN Ting5,MIAO Yungang6,LI Xiaoxue1,TAN Liping7   

  1. (1.Institute of Agricultural Product Preservation and Processing Technology,Tianjin Academy of Agricultural Sciences[National Agricultural Product Fresh⁃Keeping Engineering and Technology Research Center(Tianjin)]/National Key Laboratory of Vegetable Biological Breeding/Key Laboratory of Agricultural Product Storage and Preservation,Ministry of Agriculture and Rural Affairs/Tianjin Key Laboratory of Postharvest Physiology and Storage and Preservation of Agricultural Products,Tianjin 300384,China;2.School of Pharmaceutical Science and Technology,Tianjin University,Tianjin 300072,China;3.School of Food Science and Engineering,Tianjin University of Science and Technology,Tianjin 300457,China;4.School of Life Sciences,Tianjin University,Tianjin 300072,China;5.Fruit Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China;6.Shandong Sinocoroplast Packing Co.,Ltd.,Zibo 255130,China;7.Beijing Jintianmai International Food Co.,Ltd. ,Beijing 101407,China)
  • Received:2025-12-20 Accepted:2026-02-12 Published:2026-06-15 Online:2026-06-18

摘要: ABA响应元件结合因子(ABRE binding factor,ABF)是bZIP转录因子的一个重要亚家族,在脱落酸(Abscisic acid,ABA)依赖型和非依赖型信号通路中发挥关键作用。通过生物信息学方法,从阳光玫瑰葡萄转录组测序数据中鉴定VvABF家族成员,对其进行生物信息学分析,并结合qRT-PCR分析其在不同贮藏天数和不同二氧化氯(ClO2)浓度处理下的表达模式。结果表明,阳光玫瑰葡萄VvABF家族共有13个成员,可分为3个亚家族,每个成员含有3~6个外显子,在10条染色体上均有分布,共4对基因存在共线性关系。VvABF家族蛋白均为亲水蛋白且均不稳定,整个VvABF家族共有7个保守基序和6个保守结构域超家族,可能与非特异性丝氨酸/苏氨酸蛋白激酶如D7TPP4_VITVI等相互作用。此外,8 g/kgClO2处理下VvABF2VvABF4VvABF9VvABF13表达量显著提高。ClO2可能通过提高VvABF2VvABF4VvABF9VvABF13基因表达量来减轻采后阳光玫瑰葡萄果实褐变与衰老。

关键词: 阳光玫瑰葡萄, ABF转录因子, 采后贮藏品质, ClO2保鲜处理, 转录组测序, 生物信息学分析

Abstract: Abscisic acid(ABA)⁃responsive element(ABRE)binding factor proteins(ABF)constitute a crucial subfamily of bZIP transcription factors that play pivotal roles in both ABA⁃dependent and ABA⁃independent signaling pathways.Through bioinformatics approaches,members of the VvABF family were identified from transcriptome sequencing data of Shine⁃Muscat grape,followed by bioinformatics analysis.Their expression patterns under different storage days and varying chlorine dioxide(ClO2)concentration treatments were further investigated using qRT⁃PCR analysis.The results showed that the VvABF family comprised thirteen members dividing into three subfamilies and each member contained three to six exons,and the VvABF family members distributed across ten chromosomes with four gene pairs exhibiting collinear relationships.The VvABF family proteins were all hydrophilic and unstable.The entire VvABF family had seven conserved motifs and six conserved structural domains superfamily,which might interact with non⁃specific serine/threonine protein kinases such as D7TPP4_VITVI. Notably,8 g/kg ClO2treatment significantly increased the expression levels of VvABF2VvABF4VvABF9,and VvABF13.ClO2may mitigate post⁃harvest browning and aging of Shine⁃Muscat grape fruits by increasing the expression levels of VvABF2VvABF4VvABF9,and VvABF13genes.

Key words: Shine?Muscat grape, ABF transcription factor, Post?harvest storage quality, ClO2preservation treatment, Transcriptome sequencing, Bioinformatics analysis

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