关键词: 小麦, TaHsc70-2, 克隆, 生物信息学, 表达分析, 非生物胁迫

Abstract: The heat shock protein gene TaHsc70‑2 was cloned from the wheat cultivar Chinese Spring.Subsequently，bioinformatics analysis was performed，and its expression patterns under various hormone treatments［auxin（IAA），gibberellin（GA₃），zeatin（ZT），and abscisic acid（ABA）］and stress conditions（drought，salt，high temperature，and low temperature） were analyzed. The results showed that three TaHsc70‑2 genes，namely TaHsc70‑2‑5A，TaHsc70‑2‑5B，and TaHsc70‑2‑5D，were cloned，which encoded proteins of 648，647，and 648 amino acids，respectively. The gene structure，protein tertiary structure，and conserved motif arrangements and positions of these three genes were highly similar to those of Hsc70‑2 genes in Arabidopsis thaliana and Brachypodium distachyon.The TaHsc70‐2 protein sequences were highly conserved，and showed close phylogenetic relationships with orthologous proteins from Hordeum vulgare and Brachypodium distachyon. Protein‐protein interaction prediction analysis revealed that TaHsc70‐2 proteins had interaction relationships with proteins containing J‐domain and HATPase_c domain. The promoter regions of TaHsc70‑2 genes contained various cis‐acting elements associated with response to hormone，growth and development，and light signaling. TaHsc70‑2 genes were expressed in different tissues at various developmental stages of wheat，with relatively higher expression levels in roots.The expression of TaHsc70‑2 genes was differential under different hormone treatments，and was upregulated under ZT，high temperature，and drought treatments. It is hypothesized that TaHsc70‑2 genes play an important role in wheat growth，development，and responses to abiotic stress.

Key words: Wheat, TaHsc70?2, Cloning, Bioinformatics, Expression analysis, Abiotic stress