关键词: 鹅细小病毒, 分离鉴定, 糖基化位点, 基因重组分析

Abstract: To elucidate the genomic characteristics and genetic evolutionary patterns of the prevalent goose parvovirus（GPV） strain in Dingxing County，Hebei Province，the pathogen was isolated and identified from liver tissue samples of 35‑day‑old geese suspected of dying from gosling plague in a goose farm located in Dingxing County. The virus was inoculated into the goose embryo allantoic cavity，and the morphology of the virus particles was observed by transmission electron microscopy（TEM）. The whole genome sequence of a GPV isolate was obtained by PCR amplification and sequencing. The genome structure was further analyzed by sequence alignment，potential glycosylation sites were predicted，and recombination detection and phylogenetic analysis were carried out. The results showed that this isolate caused the production of large amounts of uric acid salts in the allantoic fluid during goose embryo culture；TEM observation revealed viral particles with a diameter of approximately 20 nm.Animal pathogenicity tests indicated a 53. 3% mortality rate in goslings. Whole genome sequencing and analysis revealed that the isolate had a genome length of 5 046 bp and was named HB‑DX strain（GenBank accession number：OR544341.1）. Sequence alignment revealed that the HB‑DX strain was similar to RC16，82‑0321，YZ99‑6 and other strains，and there were two 14 bp deletions in the UTR region at both ends of the genome. The prediction of glycosylation sites showed that the non‑structural protein 1（NS1）coding region of this strain contained 4 potential glycosylation sites，while the structural protein 1（VP1）coding region contained 5. Recombination analysis indicated that the HB‑DX strain underwent two recombination events，with the European pathogenic B strain as the major parent and the Chinese vaccine strain SYG61V as the minor parent. Phylogenetic analysis showed that the nucleotide homology of this isolate was 93.3%—99.6% with GPV branch，and 92.9%—94.4% with NGPV（novel goose parvovirus）branch，while its homology was significantly lower at 80.0%—84.1% with MDPV（muscovy duck parvovirus） branch. In conclusion，HB‑DX is a highly virulent GPV strain originated through recombination.

Key words: Goose parvovirus, Isolation and identification, Glycosylation site, Gene recombination analysis