河南农业科学 ›› 2025, Vol. 54 ›› Issue (9): 43-52.DOI: 10.15933/j.cnki.1004-3268.2025.09.005

• 作物栽培·遗传育种 • 上一篇    下一篇

MgCl2 胁迫对紫花苜蓿萌发期和苗期生长的影响及耐盐指标筛选

魏晓娜1,张晨1,祁乐萍1,沈迎芳1,2,3   

  1. (1.青海民族大学 生态环境与资源学院,青海 西宁 810007;2. 国家民委青藏高原资源化学与生态环境保护重点实验室,青海 西宁 810007;3.青海省特色经济植物高值化利用重点实验室,青海 西宁 810007)
  • 收稿日期:2025-03-17 接受日期:2025-05-07 出版日期:2025-09-15 发布日期:2025-09-12
  • 通讯作者: 沈迎芳(1987-),女,青海西宁人,副教授,博士,主要从事青藏高原植物逆境生物学研究。E-mail:syfnc@126.com
  • 作者简介:魏晓娜(1998-),女,山东淄博人,在读硕士研究生,研究方向:青藏高原植物逆境生物学。E-mail:1697652839@qq.com
  • 基金资助:
    青海省自然科学基金项目(2025-ZJ-717);青海民族大学2023年度校级规划项目(23GH12);青海省昆仑英才“中青年科j技人才”托举项目(2023QHSKXRCTJ28);青海民族大学2024年研究生创新项目(54M2024003)

Effects of MgCl2 Stress on Alfalfa Growth and Screening of Salt Tolerance Indexes at Germination and Seedling Stages

WEI Xiaona1,ZHANG Chen1,QI Leping1,SHEN Yingfang1,2,3   

  1. (1.The College of Ecological Environment and Resources,Qinghai Minzu University,Xining 810007,China;2.Key Laboratory of Resource Chemistry and Eco‑environmental Protection in Qinghai‑Tibet Plateau,State Ethnic Affairs Commission,Xining 810007,China;3.Qinghai Provincial Key Laboratory of High Value Utilization of Characteristic Economic Plants,Xining 810007,China)
  • Received:2025-03-17 Accepted:2025-05-07 Published:2025-09-15 Online:2025-09-12

摘要: :设置不同浓度(0、40、80、120、160、200 mmol/L)MgCl2溶液,研究MgCl2胁迫对紫花苜蓿种子萌发和幼苗生长、生理指标的影响,并采用灰色关联度分析法筛选耐盐指标,以期为紫花苜蓿响应MgCl2胁迫的应答机制研究及盐碱地治理提供一定的理论基础。结果表明,随着MgCl2浓度增加、处理时间延长,紫花苜蓿种子萌发、幼苗生长受到的抑制程度越来越大,在MgCl2浓度为200 mmol/L时,种子不萌发,盐害指数为1.0。紫花苜蓿种子萌发指标中与耐盐性关联较大的指标是鲜质量、发芽率和发芽指数,关联度分别为0.948、0.927和0.927;苗期生长、生理指标中与耐盐性关联较大的指标是叶绿素含量、超氧化物歧化酶(SOD)活性和过氧化氢酶(CAT)活性,关联度分别为0.941、0.920和0.917。综上,紫花苜蓿萌发期和苗期的主要耐盐指标分别为鲜质量、发芽率、发芽指数和叶绿素含量、SOD活性、CAT活性。

关键词: 紫花苜蓿, MgCl2, 种子萌发, 幼苗生长, 耐盐指标

Abstract: Different concentrations(0,40,80,120,160,200 mmol/L)of MgCl2 solution were set up to study the effects of MgCl2 stress on the seed germination,seedling growth and physiological indexes of alfalfa.The gray correlation analysis was used to screen the salt tolerance indexes,with the aim of providing a certain theoretical basis for the research on the response mechanism of alfalfa to MgCl2 stress and the governance of saline‑alkali land. The results showed that with the increase of MgCl2 concentration and treatment time,the inhibition of alfalfa seed germination and seedling growth became more and more severe.When the MgCl2 concentration was 200 mmol/L,the seeds did not germinate and the salt damage index was 1.0.Among the germination indexes of alfalfa seeds,the fresh weight,germination rate and germination index were more closely related to salt tolerance,with correlation degrees of 0.948,0.927 and 0.927,respectively.Among the seedling growth and physiological indexes,the chlorophyll content,superoxide dismutase(SOD) activity and catalase(CAT) activity were more closely related to salt tolerance,with correlation degrees of 0.941,0.920 and 0.917,respectively. In conclusion,the main salt tolerance indexes of alfalfa are fresh weight,germination rate,germination index and chlorophyll content,SOD activity,CAT activity at germination and seedling stages,respectively.

Key words: Alfalfa, MgCl2, Germination of seeds, Seedling growth, Salt tolerance indexes

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