关键词: 香菜, 秋水仙素, 染色体加倍, 四倍体诱导

Abstract: Using Australian four season large leaf coriander seeds as experimental materials，different mass fractions of colchicine（0.1%，0.2%，0.4%，0.6%，0.8%）and different time periods（2，4，6，8，10 h）were used for mutagenesis treatment to study the effect of colchicine on the induction of tetraploid coriander.The results showed that with the increase of colchicine concentration and the prolongation of treatment time，the germination rate and seedling rate of coriander seeds showed a significant downward trend.Among them，0.4% colchicine treatment for 2 h was the optimal induction condition，with the highest germination rate，seedling rate，and induction rate of 80%，60%，and 18.7%，respectively.The physiological indicators of coriander seedlings significantly improved，with chlorophyll a+b，soluble protein，and soluble sugar contents of 1.49，9.87，and 17.54 mg/g，respectively，which increased by 88.61%，58.97%，and 53.88% compared to the control.Through cytological identification，it was found that compared with diploid plants，tetraploid plants showed a significant reduction in the number of stomata per unit area of their leaves，an increase in stomata，and a doubling of the chromosome number in root tip cells（4n=44）.In addition，tetraploid coriander plants also exhibited excellent traits such as wide and thick green leaves，thicker and longer purple stems，strong aroma，and larger flowers and

Key words: Coriander, Colchicine, Chromosome doubling, Tetraploid induction