矮牵牛F3’5’H 基因在花瓣呈色中的作用解析

doi:10.15933/j.cnki.1004-3268.2021.12.013

河南农业科学 ›› 2021, Vol. 50 ›› Issue (12): 121-127.DOI: 10.15933/j.cnki.1004-3268.2021.12.013

矮牵牛F3’5’H 基因在花瓣呈色中的作用解析

符真珠¹，王锐¹，张泰然¹，王慧娟¹，高杰¹，李艳敏¹，蒋卉¹，王利民¹，袁欣¹，李彦邦²，张和臣¹

（1.河南省农业科学院园艺研究所，河南郑州 450002；2.上海交通大学农业与生物学院，上海 200240）

收稿日期:2021-08-26 出版日期:2021-12-15 发布日期:2022-01-28
通讯作者: 张和臣（1979-），男，河南濮阳人，副研究员，主要从事观赏植物花色形成及相关机理研究。E-mail：zhc5128@126.com 李彦邦（1986-），男，山东沂南人，讲师，主要从事观赏植物花色形成及细胞生物学相关研究。E-mail：yanbang.li@sjtu.edu.cn
作者简介:符真珠（1982-），女，河南信阳人，助理研究员，博士，主要从事观赏植物花色形成及组培快繁相关机理研究。 E-mail：pearlgh2005@163.com
基金资助:
河南省自然科学基金项目（212300410360）；浦江人才计划项目（19PJ1406700）

Identification of F3’5’H Gene in Petunia and the Roles in Petal Coloration

FU Zhenzhu¹，WANG Rui¹，ZHANG Tanran¹，WANG Huijuan¹，GAO Jie¹，LI Yanmin¹，JIANG Hui¹，WANG Limin¹，YUAN Xin¹，LI Yanbang²，ZHANG Hechen¹

（1.Horticultural Research Institute，Henan Academy of Agricultural Sciences，Zhengzhou 450002，China；2.School of Agriculture and Biology，Shanghai Jiao Tong University，Shanghai 200240，China）

Received:2021-08-26 Published:2021-12-15 Online:2022-01-28

摘要/Abstract

摘要： 为了揭示F3’5’H 基因对矮牵牛花瓣的呈色作用，对其进行生物信息学分析和功能验证。结果表明，矮牵牛基因组中共有HF1 和HF2 两个位点编码F3’5’H 基因。不同类型的矮牵牛具有不同的F3’5’H基因型。F3’5’H1^R27在第3个内含子上有1个dTPH9 转座子的插入，使其产生2个类型的转录本，其中1个转录本的非正常编码是矮牵牛花瓣呈现粉色的主要原因。在矮牵牛M1×R27中超表达不同类型的F3’5’H 基因，除F3’5’H1^R27外，其他基因型都能正常行使分子功能，促使转基因后代的矮牵牛花瓣呈现紫色。

关键词: 矮牵牛, F3’5’H, 花色, 分子机制

Abstract: In order to reveal the coloration effect of F3’5’H gene on petunia petals，bioinformatics analysis and functional identification of F3’5’H gene were conducted.The results showed that there were two gene loci of HF1 and HF2 encoding F3’5’H in petunia genome and different species of petunia contained different F3’5’H genotypes. Among them，F3’5’H1^R27 had a dTPH9 transposon insertion in the third intron，which caused two types of transcripts.The abnormal coding of one transcript was the main cause of petunia pink petals.All genotypes could perform molecular functions normally except for F3’5’H1^R27 by overexpressing different types of F3’5’H genes in M1×R27，which promoted the petunia petals of transgenic progeny to be purple.

Key words: Petunia, F3’5’H, Flower color, Molecular mechanism

中图分类号:

S681.6

符真珠, 王锐, 张泰然, 王慧娟, 高杰, 李艳敏, 蒋卉, 王利民, 袁欣, 李彦邦, 张和臣. 矮牵牛F3’5’H 基因在花瓣呈色中的作用解析[J]. 河南农业科学, 2021, 50(12): 121-127.

FU Zhenzhu, WANG Rui, ZHANG Tanran, WANG Huijuan, GAO Jie, LI Yanmin, JIANG Hui, WANG Limin, YUAN Xin, LI Yanbang, ZHANG Hechen. Identification of F3’5’H Gene in Petunia and the Roles in Petal Coloration[J]. Journal of Henan Agricultural Sciences, 2021, 50(12): 121-127.

图/表 4

参考文献

[1] VANDENBUSSCHE M, CHAMBRIER P, BENTO SUZANNE S, et al.Petunia, your next supermodel[J].Frontiers in Plant Science,2016,7:72.

[2] MORITA Y, HOSHINO A. Recent advances in flower color variation and patterning of Japanese morning glory and petunia[J]. Breeding Science,2018,68(1):128-138.

[3] KISHIMOTO S, ODA-YAMAMIZO C, OHMIYA A. Comparison of petunia and calibrachoa in carotenoid pigmentation of corollas[J]. Breeding Science,2019,69(1):117-126.

[4] KISHIMOTO S,ODA-YAMAMIZO C,OHMIYA A. Heterologous expression of xanthophyll esterase genes affects carotenoid accumulation in petunia corollas[J]. Scientific Reports,2020,10(1):1299.

[5] YUKIHISA K,MASAKO F M,YUKO F, et al. Engineering of the rose flavonoid biosynthetic pathway successfully generated blue-hued flowers accumulating delphinidin[J]. Plant and Cell Physiology,2007,48(11):1589-1600.

[6] TANAKA Y, TSUDA S, KUSUMI T. Metabolic engineering to modify flower color[J]. Plant and Cell Physiology,1998,39:1119-1126.

[7] 张泰然,张和臣,武荣花.蓝色花形成分子机理研究进展[J].植物学报,2020,55(2):216-227.

ZHANG T R,ZHANG H C,WU R H. Recent advances on blue flower formation[J].Chinese Bulletin of Botany,2020,55(2):216-227.

[8] NODA N. Recent advances in the research and development of blue flowers[J]. Breeding Science,2018, 68:79-87.

[9] TANAKA Y,BRUGLIER A. Flower colour and cytochromes P450[J].Philosophical Transactions of the Royal Society,2013,368(1612):20120432.

[10] SASAKI N, NAKAYAMA T. Achievements and perspectives in biochemistry concerning anthocyanin modification for blue flower coloration[J].Plant and Cell Physiology,2015,56(1): 28-40.

[11] BRUGLIERA F,TAO G Q,TEMS U,et al. Violet/blue chrysanthemums-metabolic engineering of anthocyanin biosynthetic pathway results in novel petal colours[J]. Plant and Cell Physiology,2013,54(10):1696-1710.

[12] BOMBARELY A, MOSER M, AMRAD A, et al. Insight into the evolution of the Solanaceae from the parental genomes of Petunia hybrida[J]. Nature Plants,2016,2(6):16074.

[13] CHEN S,MATSUBARA K,OMORI T, et al. Phylogenetic analysis of the genus Petunia(Solanaceae) based on the sequence of the Hf1 gene[J]. Journal of Plant Research,2007,120:385-397.

[14] SEITZ C, AMERES S, SCHLANGEN K,et al. Multiple evolution of flavonoid 3’,5’-hydroxylase[J]. Planta,2015,242:561-573.

[15] ZHANG H,KOES R,SHANG H,et al. Identification and functional analysis of three new anthocyanin R2R3-MYB genes in Petunia[J].Plant Direct,2019,3(1):e00114.

[16] 戴思兰,洪艳.基于花青素苷合成和呈色机理的观赏植物花色改良分子育种[J].中国农业科学,2016,49(3):529-542.

DAI S L, HONG Y. Molecular breeding for flower colors modification on ornamental plants based on the mechanism of anthocyanins biosynthesis and coloration[J]. Scientia Agricultura Sinica,2016, 49(3):529-542.

[17] KOES R,VERWEIJ W,QUATTROCCHIO F. Flavonoids:A colorful model for the regulation and evolution of biochemical pathways[J].Trends in Plant Science,2005,10(5):236-242.

[18] ISHIGURO K, TANIGUCHI M, TANAKA Y. Functional analysis of Antirrhinum kelloggii flavonoid 3’ -hydroxylase and flavonoid 3’,5’ -hydroxylase genes; critical role in flower color and evolution in the genus Antirrhinum[J]. Journal of Plant Research,2012,125:451-456.

[19] NAKATSUKAT,NISHIHARA M,MISHIBA K,et al. Two different transposable elements inserted in flavonoid 3’,5’-hydroxylase gene contribute to pink flower coloration in Gentiana scabr[J]. Molecular Genetics and Genomics,2006,275:231-241.

[20] VIKHOREV A, STRYGINA K, KHLESTKINA E. Duplicated flavonoid 3’-hydroxylase and flavonoid 3’,5’-hydroxylase genes in barley genome[J]. PeerJournal,2019,7:e6266.

[21] QUATTROCCHIO F, WING J, VANDER W K, et al. Molecular analysis of the anthocyanin2 gene of Petunia and its role in the evolution of flower color[J]. The Plant Cell,1999,11:1433-1444.

[22] ALBERT N, DAVIES K, LEWIS D, et al. A conserved network of transcriptional activators and repressors regulates anthocyanin pigmentation in eudicots[J]. The Plant Cell, 2014,26(3):962-980.

[23]TSUDA S, FUKUI Y, NAKAMURA N, et al. Flower color modification of Petunia hybrida commercial varieties by metabolic engineering[J]. Plant Biotechnology, 2004,21(5):377-386.

矮牵牛F3’5’H 基因在花瓣呈色中的作用解析

Identification of F3’5’H Gene in Petunia and the Roles in Petal Coloration

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 4

参考文献

相关文章 15

编辑推荐

Metrics

[1]	张林霞, 张蔚, 张书婷, 孙苗苗, 张晓敏, 李志能, 刘国锋. 矮牵牛花朵大小及相关性状遗传分析[J]. 河南农业科学, 2024, 53(2): 118-127.
[2]	方宇辉, 韩留鹏, 华夏, 赵明忠, 郭瑞, 齐学礼, 胡琳. 小麦抗寒性研究进展[J]. 河南农业科学, 2022, 51(4): 1-10.
[3]	蒋朵朵, 李林. 2 种茄科植物花色变化的生理生化机制[J]. 河南农业科学, 2021, 50(8): 133-145.
[4]	宋申, 单守明, 李倩, 刘思凡. 库源关系对北玫葡萄果实花色苷成分积累及品质的影响[J]. 河南农业科学, 2021, 50(6): 109-115.
[5]	王平利, 夏璐, 魏战勇. DNA 病毒基因组复制与宿主细胞DDR 信号网络互作的研究进展[J]. 河南农业科学, 2021, 50(12): 1-9.
[6]	赵峻丽, 陈雯, 石敏. CBF在植物低温胁迫响应中的激活与调控研究进展[J]. 河南农业科学, 2020, 49(4): 1-6.
[7]	蒋卉, 袁欣, 冯乃馨, 张晶, 李艳敏, 符真珠, 高杰, 董晓宇, 王慧娟, 王利民, 张和臣. 矮牵牛同源多倍体诱导及其初期表型差异分析[J]. 河南农业科学, 2019, 48(9): 111-116.
[8]	张曼，郑云冰，范可可，陈军洲，尚玉萍，刘艺平，贺丹. 不同时期观赏海棠叶色和花色变化规律研究[J]. 河南农业科学, 2019, 48(5): 106-112.
[9]	程楠，项黛徽，沈雅园，付建新，张超. 桂花β-胡萝卜素羟化酶基因的克隆与序列分析[J]. 河南农业科学, 2019, 48(1): 99-104.
[10]	王阳，吴亚蓓，齐睿，田耕，白雪，李永华，张开明. 低温下不同H₂O₂抑制剂对四季秋海棠花色素苷合成的影响[J]. 河南农业科学, 2018, 47(11): 110-115.
[11]	陈震，郑洋，杨习武，吕丹丹，徐春丽，朱素琴. 植物体内ABA水平的动态调节机制研究进展[J]. 河南农业科学, 2016, 45(12): 1-6.
[12]	张强，张晓伟，原玉香，姚秋菊，赵艳艳，蒋武生，魏小春，王志勇. 甘蓝Ogura胞质雄性不育系发生同源异型转化的分子机制研究[J]. 河南农业科学, 2015, 44(5): 111-116.
[13]	岳桦，于淼. 卫矛叶片花色苷的提取剂稳定性研究[J]. 河南农业科学, 2014, 43(4): 110-113.
[14]	刘会超;贾文庆;尤扬;周艳霞. 连翘花色苷的提取及其稳定性研究[J]. 河南农业科学, 2010, 39(6): 121-127.
[15]	刘海英;崔长海;王改红;刘歆;王立云;高晗. 氨卞西林钠和马来酰肼对菊花形态、花色和花期的影响[J]. 河南农业科学, 2008, 37(12): 107-110.