河南农业科学 ›› 2021, Vol. 50 ›› Issue (9): 9-15.DOI: 10.15933/j.cnki.1004-3268.2021.09.002

• 作物栽培·遗传育种 • 上一篇    下一篇

玉米籽粒相关性状的QTL 定位

王新涛1,李保叶1,杨青2,代资举3,郝俊杰1   

  1. (1. 河南省农业科学院植物保护研究所,河南郑州450002;2. 河南省农业科学院芝麻研究中心,河南郑州 450002;3. 河南省作物分子育种研究院,河南郑州450002)
  • 收稿日期:2021-05-20 出版日期:2021-09-15 发布日期:2021-10-12
  • 通讯作者: 郝俊杰(1978-),男,河南登封人,研究员,博士,主要从事玉米病害研究。E-mail:haojjds@163.com
  • 作者简介:王新涛(1983-),男,河南辉县人,助理研究员,博士,主要从事玉米遗传育种研究。E-mail:tao-450002@163.com
  • 基金资助:
    河南省科技攻关计划项目(192102110026,202102110155);河南省农业科学院科研发展专项(2020CY011,2020YQ33)

QTLs Mapping for Traits Related with Kernel in Maize

WANG Xintao1,LI Baoye1,YANG Qing2,DAI Ziju3,HAO Junjie1   

  1. (1.Plant Protection Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;2.Sesame Research Center,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;3.Henan Academy of Crop Molecular Breeding,Zhengzhou 450002,China)
  • Received:2021-05-20 Published:2021-09-15 Online:2021-10-12

摘要: 为探究玉米籽粒百粒质量、粒长和粒宽的遗传机制,以郑58×D863F构建的包含241个家系的重组自交系(RIL)群体为材料,进行2 a不同环境下的百粒质量、粒长和粒宽QTL定位分析。结果表明,玉米百粒质量、粒长和粒宽在RIL群体中表现出连续变异,呈正态分布,且性状间呈极显著相关,属于典型的数量性状。在2 a不同环境下共检测到14个QTL,其中,控制百粒质量、粒长和粒宽的QTL分别有6个、3个和5个,分布在第2、3、4、7染色体上,表型贡献率介于6.42%~13.41%。其中,位于第2染色体的控制百粒质量的QTL q100 KW2 连续2 a 的表型贡献率均大于10%,为主效QTL;在第4 染色体umc1891—mmc0371区间内同时检测到控制百粒质量、粒长和粒宽的QTL,这些QTL可能包含调控籽粒性状的关键基因。

关键词: 玉米, 重组自交系群体, QTL, 百粒质量, 粒长, 粒宽

Abstract: In order to study the genetic mechanism of 100⁃kernel weight,kernel length and kernel width in maize,a recombinant inbred line(RIL)population including 241 lines derived from Zheng 58×D863F was used as material,and QTLs for 100⁃kernel weight,kernel length and kernel width were studied from 2018 to 2019 under two different environments. In RIL population,100⁃kernel weight,kernel length and kernel width showed continuous variations with a normal distribution and significant correlations,and belonged to typical quantitative traits. A total of 14 QTLs were detected in two years under two different environments. The numbers of QTLs for 100⁃kernel weight,kernel length and kernel width were 6,3 and 5,respectively. These QTLs were detected on chromosomes 2,3,4 and 7,and the PVE(phenotypic variance explained)values were 6. 42%—13. 41%. The PVE values of major QTL(q100KW2)for 100⁃kernel weight detected on chromosome 2 were above 10% under two environments. The QTLs for 100⁃kernel weight,kernel length and kernel width were detected in umc1891—mmc0371 on chromosome 4,which might contain key genes regulating grain traits.

Key words: Maize, RIL population, QTL, 100?kernel weight, Kernel length, Kernel width

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