关键词: OsPIL15, 磷, 氮, 水稻, 吸收, 转运

Abstract: To analyze the molecular mechanism of rice(Oryza sativa)phytochrome interacting factors-like 15 gene(OsPIL15)regulating rice phosphorus and nitrogen uptake in rice,firstly,the response of OsPIL15 gene to low phosphorus was analyzed by using Transcriptome ENcyclopedia of Rice database, then,Nipponia(Nip) and Ospil15 mutant were cultured under normal phosphorus (300 μmol/L NaH₂PO₄)and low phosphorus (10 μmol/L NaH₂PO₄) nutrient solution,the root and shoot biomass,phosphorus and nitrogen contents of rice seedlings were measured,and the absorption efficiency,transport coefficient and utilization efficiencies of phosphorus and nitrogen were calculated.The results showed that OsPIL15 responded to low phosphorus stress,the decrease ranges of root and shoot length of Ospil15 mutant were less than those of Nip under low phosphorus condition,and the decrease range of dry shoot weight of Ospil15 mutant was basically the same as that of Nip,but the decrease range of dry root weight of Ospil15 was less than that of Nip.Under normal phosphorus condition,the phosphorus content in root of Ospil15 mutant was significantly higher than that of Nip,but the phosphorus content in shoot of Ospil15 mutant was not significantly different from that of Nip. Under low phosphorus condition,the phosphorus contents in root and shoot of Ospil15 mutant were significantly higher than those of Nip.The phosphorus uptake efficiencies of Ospil15 mutant were higher than those of Nip under normal phosphorus and low phosphorus conditions,the phosphorus transport coefficient of Ospil15 mutant was lower than that of Nip under normal phosphorus condition,and higher than that of Nip under low phosphorus condition.Ospil15 mutant also had an effect on rice nitrogen uptake.Under normal phosphorus condition,the nitrogen content in shoot of Ospil15 mutant was significantly higher than that of Nip,but nitrogen content in root of Ospil15 mutant was not significantly different from Nip.Under low phosphorus condition,the nitrogen content in root was significantly higher than that of Nip,but there was no significant difference in shoot.The nitrogen uptake efficiencies of Ospil15 mutant were higher than those of Nip under normal phosphorus and low phosphorus conditions,and the nitrogen transport coefficient was lower than that of Nip under low phosphorus condition.The phosphorus and nitrogen utilization efficiencies of Ospil15 mutant were higher than those of Nip under both normal and low phosphorus conditions.In summary,the Ospil15 mutant increased the absorption and utilization of phosphorus and nitrogen in rice.Under low phosphorus stress,the OsPIL15 mutant increased the transport of phosphorus from root to shoot,and inhibited the transport of nitrogen from root to shoot.The results showed that OsPIL15 gene negatively regulated the uptake and utilization of phosphorus and nitrogen in rice.

Key words: OsPIL15, Phosphorus, Nitrogen, Rice, Absorption, Transportion