关键词: 艾, 表型, 矿物元素, 药用成分, 综合评价

Abstract: In order to study the phenotypic diversity of germplasm resources,and clarify the differences of mineral elements and main medicinal components in different phenotypes of Artemisia argyi,in the present study,inductively coupled plasma mass spectrometry( ICP-MS) was used to detect the contents of 35 mineral elements, including Be, Na, Mg, Al, K, etc.in 4 different phenotypes of A.argyi, and then comprehensive evaluation was carried out by principal component analysis and factor analysis.In addition,high-performance liquid chromatography(HPLC) was used to detect the contents of isotendin,chlorogenic acid and isochlorogenic acid B.The results showed that the contents of 32 mineral elements were significantly different in 4 phenotypes of A.argyi(P<0.05).With the content of each element as a variable,a total of 4 principal components were extracted, and the variance contribution rates were 70.092%,11.055%,9.485%,and 3.743%,respectively.According to the calculation rule of adding the product of the load of each principal component (F₁—F₄) and the variance contribution rate, the comprehensive evaluation function was obtained as F=0.700 9F₁+0.110 6F₂+0.094 9F₃+0.037 4F₄.Accordingly,the phenotype 1 and phenotype 2 had the highest scores.The content of eupatilin in phenotype 1 was the highest(3. 01 mg/g),which was significantly higher than that of phenotype 2 and phenotype 3,but the difference between phenotype 4 and phenotype 1 was not significant;the contents of chlorogenic acid in phenotype 1 and phenotype 3(3.56 mg/g and 4.59 mg/g, respectively)were significantly higher than those in phenotype 2 and 4;The similar result was found for isochlorogenic acid B in phenotype 1 and phenotype 3 ( 4.91 mg/ g and 5.10 mg/g, respectively) when compared with phenotype 2,but not with phenotype 4. These results indicate that phenotype 1 can be the priority in the selection of 4 cultivars based on the comprehensive consideration of the content of mineral elements and
medicinal components.

Key words: Artemisia argyi, Phenotype, Mineral element, Medicinal component, Comprehensive evaluation