关键词: 猕猴桃； 腐烂病； 病原菌鉴定； 序列分析； 致病性检测, 拟茎点霉菌； 镰刀菌； 黄瓜织球壳菌

Abstract: In order to clarify the types of the pathogens of fruit rot disease of main kiwifruit varieties in Guizhou Province,and provide theoretical basis for the prevention and control of field fruit diseases,the diseased fruits of Hongyang and Guichang kiwifruit collected in Shuicheng County and Xiuwen County of Guizhou Province from 2018 to 2019 were used as experimental materials.The pathogens were identified through pathogen isolation,morphological observation,molecular detection and pathogenicity detection.The phylogenetic analysis of pathogen species based on their sequences were carried out.The results showed that four kinds of pathogens including Phomopsis cauloides, Fusarium, Alternaria alternata,Plectosphaerella cucumerina were isolated, and their detection rates were 10%, 60%, 20%, 10%respectively. The fruit disease of kiwifruit caused by the infection of Plectosphaerella cucumerina was reported for the first time in China.

Key words: Kiwifruit, Canker disease, Pathogen identification, Sequence analysis, Pathogenicity test, Phomopsis cauloides, Fusarium, Plectosphaerella cucumerina