关键词: 牡丹, 油用凤丹, 杂交授粉, 混合授粉, 促花粉萌发剂

Abstract: Both the suitable pollination cultivars and pollen germination promoting reagents were screened in this paper to improve the yield of oil Fengdan. Pollens of 16 representative ornamental peony cultivars and Fengdan from four plantations(Kaiyuan campus and Zhoushan campus of Henan University of Science and Technology,Luoyang East Garden and Songxian KuquTownship) were selected as the male parents,and Fengdan from four different altitude bases[Zhoushan campus of Henan University of Science and Technology(altitude of 197—215 m),Luoyang East Garden(altitude of 119—121 m),Songxian Kuqu Township(altitude of 369—371 m) and Luanchuan Diaoyutai Village(altitude of 703—710 m)] were selected as the female parents. Furthermore,the mixed pollens with different pollen cultivars were selected randomly to spray them on Fengdan in blooming stage,subsequently the seed setting rate,grain number,100-grain weight and the average yield of each flower were measured and compared.The results showed that male parent for the cross pollination combination with the highest seed setting rate and the highest average yield of each flower in Zhoushan campus was Fengdan cultivar from Kaiyuan campus,and the seed setting rate reached up to 96.67%,which was 1.4 times of the natural control,while the average yield of each flower was 16.21 g,which was 1. 8 times of the natural control.In Songxian Kuqu Village,the male parent for cross pollination combination with the highest seed setting rate was Fengdan cultivar from Zhoushan campus, and the seed setting rate was 60.00%, which was 1.1 times of the natural control,while Japan Crown got the highest average yield of each flower of 3.47 g as the male parent for cross pollination combination,which was 6.1 times of the natural control.In East Garden,the male parent for cross pollination combination with the highest seed setting rate was Riyuejin cultivar, which was 96.67% and 1.5 times of the natural control, and Roufurong got the highest average yield of each flower,which was 9.01 g and 1.5 times of the natural control. In Luanchuan Diaoyutai Village,the male parent for cross pollination combination with the highest seed setting rate was Fengdan cultivar from Zhoushan campus,which was 100.00% and 1.3 times of the natural control, and Riyuejin got the highest average yield of each flower,which was 12.70 g and 2.7 times of the natural control.The results of mixed pollination test showed that the best male parent combination with the highest seed setting rate was Fengdan(East Garden)+Wujinyaohui+Roufurong,which was 80.00% and 1.2 times of the natural control,while another combination with the highest average yield of each flower was Wujinyaohui+Riyuejin+Luoyanghong+Fengdan (East Garden), which was 17.02 g and 2.8 times of the natural control.The results of pollen germination promoting reagent test showed that the boric acid(0.08 g/L) or sucrose(150 g/L)+boric acid(0.08 g/L) PEG-6000(200 g/L) could improve the seed setting rate and yield of Fengdan as spraying regent.Therefore it was suggested that Riyuejin,Roufurong,Japan crown and Fengdan cultivars from different altitude bases could be good male parents, and the comprehensive treatments such as mixed pollination or spraying pollen germination promoting agent could also be used to improve the yield of oil Fengdan.

Key words: Paeonia ostii, Oil Fengdan, Hybrid pollination, Mixed pollination, Pollen germination promoting agent