关键词: 天麻, 病害, 多样性, 真菌群落, 高通量测序

Abstract: The study of the differences in fungal community structure in healthy and diseased Gastrodia elata provides clues for the investigation of the mechanism of Gastrodia disease.Traditional culture method and high-throughput sequencing technology were used to analyze the changes of fungal commu ity structure in healthy and diseased plants tubers.The results of culture analysis showed that the healthy and diseased Gastrodia tubers fungi covered 3 phyla and 14 genera.Among them, there were 8 genera including Trichoderma and Talaromyces in healthy Gastrodia.There were 10 genera including Cladosporium,Alternaria and Fusarium in the Gastrodia elata strain.A total of 1 692 OTUs(operational taxonomic units) were obtained.The classification of species showed that at the phylum level,the main groups of fungi in the healthy and diseased groups of Gastrodia were basically the same,mainly including Ascomycota and Basidiomycota,but there were obvious differences in the relative abundance of fungi in healthy and diseased tubers of Gastrodia elata.At the genus level,the composition and relative abundance of fungi healthy and diseased Gastrodia were very different.Among them, Ilyonectria, Helotiales and Fusarium may be the pathogenic bacteria group that caused diseases of Gastrodia.It can be seen that both the traditional culture method and the high-throughput sequencing technology show that there are significant differences in the fungal community structure between healthy and diseased Gastrodia elata.The occurrence of Gastrodia elata disease is related to the change of the fungal community structure in Gastrodia elata,suggesting that it can be prevented by adding superior probiotics in the early stage.

Key words: Gastrodia elata, Disease, Diversity, Fungal community, High-throughput sequencing