关键词: 玉米, 拟南芥, 光敏色素C, 去黄化反应, 避荫性

Abstract: In order to study the similarities and differences of PHYC functions between maize and Arabidopsis and the functions in photomorphogenesis and shade avoidance of two maize PHYC genes,we cloned two maize PHYC genes,analyzed the functional domains of maize PHYC and phylogenetic tree of PHYC proteins maize and common plant species by bioinformatics,and verified the functions of two maize PHYC genes in Arabidopsis by transgenic techniques.The results showed that the coding regions of ZmPHYC1 and ZmPHYC2 from maize inbred line B73 were cloned.The coding region length was 3 405 bp,and encoded a protein with 1 135 amino acid residues with molecular weight of 126.14 ku and 126.07 ku.Similar to the PHYC proteins of Arabidopsis and rice,ZmPHYC1 and ZmPHYC2 contained a PAS_2domain,a GAF domain,a PHY domain,two PAS repeat domains,a histidine kinase A domain and a histidine kinase like-ATPase domain. Amino acid sequence homology analysis showed that ZmPHYC1 and ZmPHYC2 had the highest homology with the sorghum PHYC protein.ZmPHYC1 and ZmPHYC2 could not only complement long hypocotyl phenotype caused by Arabidopsis phyC-2 mutant under red light,but also promote the de-etiolation of seedlings under continuous red and blue light conditions,and inhibit the elongation of hypocotyl under shade condition.For promotion of seedling de-etiolation and inhibition of shade avoidance,ZmPHYC1 play more important role than ZmPHYC2.

Key words: Zea mays, Arabidopsis thaliana, Phytochrome C, De-etiolation, Shade avoidance