河南农业科学 ›› 2020, Vol. 49 ›› Issue (3): 47-53.DOI: 10.15933/j.cnki.1004-3268.2020.03.007

所属专题: 中草药种子引发专题

• 作物栽培·遗传育种 • 上一篇    下一篇

不同试剂及温度对解除滇重楼种子休眠的影响

汪佳维,王华磊,赵致,刘红昌,罗春丽,陈松树   

  1. (贵州大学 农学院/贵州省药用植物繁育与种植重点实验室,贵州 贵阳 550025)
  • 收稿日期:2019-08-31 出版日期:2020-03-15 发布日期:2020-03-15
  • 通讯作者: 王华磊(1979-),男,山东兖州人,教授,博士,主要从事药用植物栽培研究。E-mail:3027865861@163.com
  • 作者简介:汪佳维(1994-),女,贵州遵义人,在读硕士研究生,研究方向:药用植物栽培。E-mail:854473604@qq.com
  • 基金资助:
    贵州省生物学一流学科建设项目(GNYL[2017]009);贵州省药用植物繁育与种植人才基地项目(黔人领发[2013]15号);贵州省特色粮油作物栽培与生理生态研究科技创新人才团队项目(黔科合平台人才[2019]5613号)

Effects of Different Reagents and Temperature on Removing Seed Dormancy of Paris polyphylla var.yunnanensis

WANG Jiawei,WANG Hualei,ZHAO Zhi,LIU Hongchang,LUO Chunli,CHEN Songshu   

  1. (College of Agriculture,Guizhou University/Guizhou Key Laboratory of Propagation and Cultivation on Medicinal Plants,Guiyang 550025,China)
  • Received:2019-08-31 Published:2020-03-15 Online:2020-03-15

摘要: 为筛选解除滇重楼种子休眠的最佳方法,分别研究了不同质量浓度赤霉素(GA)、氟啶酮(FL)浸种和不同层积温度对滇重楼种子发芽和胚率变化的影响。结果表明,GA单独处理、GA+FL组合、恒温20℃沙藏都可有效打破滇重楼种子的休眠,其中GA单独处理下发芽情况最好的是300 mg/L GA处理,其发芽率和发芽势分别为79.00%、54.33%;GA+FL组合处理下300 mg/L GA+10 mg/L FL处理发芽率最高(81.33%),300 mg/L GA+0 mg/L FL处理发芽势最高(54.33%),200 mg/LGA+10 mg/L FL处理烂种率最高(4.33%);不同沙藏温度处理中恒温20℃沙藏处理下滇重楼种子胚率增长最快。综合滇重楼种子的发芽情况来看,GA+ FL组合及恒温20 ℃沙藏这2种处理解除滇重楼种子休眠的效果优于其他处理,即300 mg/L GA+10 mg/L FL为最佳浸种处理,恒温20℃为最佳沙藏温度。

关键词: 滇重楼;种子休眠, 氟啶酮;赤霉素;层积处理;发芽率, 发芽势, 胚率

Abstract: The effects of different concentrations of gibberellin(GA),fluridone(FL) to soak the seeds and different stratification temperature on seed germination and embryo rate of Paris polyphylla var.yunnanensis were studied respectivly to screen the best way to revive the seed of Paris polyphylla var.yunnanensis.The results showed that GA treatment alone,GA+FL combination and sand storage at constant temperature of 20℃ could effectively break the dormancy of the seeds.Among them,the best germination was at 300 mg/L with GA treatment alone, and the germination rate and germination potential were 79.00% and 54.33% respectively.The highest germination rate was 81.33% with GA+FL combination(300 mg/L GA+10 mg/L FL),and the highest germination potential was 54.33% with 300 mg/L GA+0 mg/L FL,and the rotten seed rate was 4.33% with 200 mg/L GA+10 mg/L FL.The seed embryo rate of Paris polyphylla var.yunnanensis increased fastest under constant temperature of 20 ℃ in different temperatures with sand storage.By all accounts of the seeds germination of Paris polyphylla var.yunnanensis,the effects of GA+FL combination and constant temperature of 20℃ sand storage were better than other treatments.That is to say,300 mg/L GA+10 mg/L FL is the optimal seed soaking combination,and constant temperature of 20℃ is optimal for sand storage.

Key words: Paris polyphylla var.yunnanensis, Seeds dormancy, Fluridone, Gibberellin, Stratification;Germination rate, Germination potential, Embryo rate

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