河南农业科学 ›› 2019, Vol. 48 ›› Issue (10): 147-154.DOI: 10.15933/j.cnki.1004-3268.2019.10.022

• 畜牧·兽医 • 上一篇    下一篇

6种中药体外抗猪繁殖与呼吸综合征病毒的作用

刘云1,2，朱善元1，龚祝南2，秦枫1，夏文龙1,吴双1，吴晓洁1   

  1. (1.江苏农牧科技职业学院/江苏省兽用生物制药高技术研究重点实验室，江苏 泰州 225300；2.南京师范大学 生命科学学院，江苏南京 210000）
  • 收稿日期:2019-03-31 出版日期:2019-10-15 发布日期:2019-10-15
  • 通讯作者: 秦 枫（1979-），女，山东烟台人，副教授，〖JP3〗在读博士研究生，主要从事天然药物与动物疾病防治研究与教学工作。E-mail:qinancy922@163.com
  • 作者简介:刘 云（1992-），女，江苏泰州人，硕士，主要从事中兽药抗病毒研究。E-mail:837917672@qq.com
  • 基金资助:
    江苏省高等学校自然科学研究面上项目（18KJB230002）；江苏高校“青蓝工程”资助项目（00000217008）；江苏农牧科技职业学院“凤凰人才工程”项目（10434014001）；江苏省大学生创新项目（201912806026Y）

Effects of Six Traditional Chinese Medicines against Porcine Ｒeproductive and Ｒespiratory Syndrome Virus in Vitro

 LIU Yun1,2，ZHU Shanyuan1，GONG Zhunan2，QIN Feng1，XIA Wenlong1，WU Shuang1，WU Xiaojie1   

  1.  ( 1.Jiangsu Agri-animal Husbandry Vocational College/Jiangsu Provincial Key Laboratory of Veterinary Bio-pharmaceutical High-tech Ｒesearch,Taizhou 225300,China; 2.College of Life Sciences,Nanjing Normal University,Nanjing 210000,China)
  • Received:2019-03-31 Published:2019-10-15 Online:2019-10-15

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摘要： 为研究荆芥、委陵菜、啤酒花、翻白草、百部和虎杖6种中药体外抗猪繁殖与呼吸综合征病毒（PRRSV）的作用，建立了Marc-145细胞模型，在研究其对Marc-145细胞最大安全质量浓度的基础上，结合细胞形态观察和MTT法，对PRRSV进行阻断、抑制、灭活3种作用方式的测定。结果显示，荆芥、委陵菜、啤酒花、翻白草、百部和虎杖对Marc-145细胞的最大安全质量浓度分别为0.25 mg/mL、1.25 mg/mL、0.63 mg/mL、0.31 mg/mL、1.56 mg/mL和0.47 mg/mL。在体外抗PRRSV作用的试验中，从细胞形态观察发现，委陵菜和虎杖药物组均未出现明显的细胞病变，其余4种药物组都出现了不同程度的细胞病变；从对PRRSV的抑制率来看，荆芥对PRRSV的阻断、抑制和直接灭活作用的最高抑制率分别为80.6%、52.2%和56.5%，委陵菜3种作用方式对PRRSV的最高抑制率分别为115.8%、93.0%和125.4%，啤酒花3种作用方式对PRRSV的最高抑制率分别为61.5%、32.0%和82.4%，翻白草3种作用方式对PRRSV的最高抑制率分别为15.6%、129.4%和57.7%，百部3种作用方式对PRRSV的最高抑制率分别为120.2%、61.8%和71.9%，虎杖3种作用方式对PRRSV的最高抑制率分别为110.4%、94.3%和130.8%。综合细胞形态观察和抑制率结果，委陵菜和虎杖抗PRRSV的效果最好，其次是荆芥和百部。

关键词: 猪繁殖与呼吸综合征病毒, 中药, 抗病毒； 体外； 细胞病变； 抑制率

Abstract:  In order to study the effects of six traditional Chinese medicines，such as Nepeta cataria L.,Potentilla chinensis Ser.,Humulus lupulus Linn.,Potentilla discolor Bge.,Stemona japonica(Blume) Miq.and Ｒeynoutria japonica Houtt.,against porcine reproductive and respiratory syndrome in vitro,the Marc 145 cell model was established in this experiment.Based on the study of their maximum safe mass con centration on Marc-145 cells,combined with the observation of cell morphology and MTT method,the three actions of blocking,inhibiting and inactivating the virus were determined,respectively.The results showed that the maximum safe mass concentrations of Nepeta cataria L.,Potentilla chinensis Ser.,Humu lus lupulus Linn.,Potentilla discolor Bge.,Stemona japonica(Blume) Miq.and Ｒeynoutria japonica Houtt.on Marc-145 cells were 0.25 mg/mL，1.25 mg/mL，0.63 mg/mL，0.31 mg/mL，1.56 mg/mL， and 0.47 mg/mL.In the experiment of anti-PＲＲSV effect in vitro，it was found from the cell morphology observation that under the three modes of action on PＲＲSV，the cells treated by Potentilla chinensis Ser.and Ｒeynoutria japonica Houtt. had no obvious lesions，and the treatment of other four drugs showed dif ferent degrees of lesions.From the inhibition rate of PＲＲSV，the highest inhibition rates of blocking，inhi bition and direct inactivation of Nepeta cataria L.were 80.6%，52.2% and 56.5%,respectively.The highest inhibition rates of PＲＲSV by three modes of action of Potentilla chinensis Ser.were 115.8%,93.0% and 125.4%,respectively.The highest inhibition rates of PＲＲSV in the three modes of action of Humulus lupulus Linn.were 61.5%，32.0% and 82.4%，respectively.The highest inhibition rates of PＲＲSV in the three modes of action of Potentilla discolor Bge.were 15.6%,129.4% and 57.7%,re spectively.The highest inhibition rates of PＲＲSV in the three modes of action of Stemona japonica (Blume) Miq. were 120.2%，61.8% and 71.9%,respectively.The highest inhibition rates of PＲＲSV in the three modes of action of Ｒeynoutria japonica Houtt.were 110.4%，94.3% and 130.8%,respective ly.Combined with morphological observation and inhibition rate results，Potentilla chinensis Ser.and Ｒey noutria japonica Houtt.had the best anti-PＲＲSV effect，followed by Nepeta cataria L.and Stemona japon ica(Blume) Miq．

Key words: Porcine reproductive and respiratory syndrome virus, Traditional Chinese medicine, Antivirus; In vitro, Cytopathy, Inhibition rate

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