关键词: 畜禽清肺颗粒, 响应面法, 中药发酵, 制备工艺

Abstract: In order to solve the technical problems of low extraction efficiency of traditional Chinese herb，single factor experiment combined with response surface methodology was carried out to improve thepreparation process of livestock and poultry Qingfei granules，including fermentation extraction，extract extraction，alcohol precipitation，granule formulation，granulation，drying and straightening granulation，etc.，and the content of active ingredients of the modified livestock and poultry Qingfei granules was determined.The results showed that the optimal preparation conditions of livestock and poultry Qingfei granules were as follows：inoculation ratio was 3%，culture temperature was 37℃ and culture time was 72 h；the fermented traditional Chinese medicine was extracted twice with 8 times of water，1.5 h each time；the extractum was soaked in 70% ethanol under the condition of relative density of 1.35 for 24 h；the extractum was mixed with auxiliary materials at a ratio of 1∶7.6，and the auxiliary materials were lactose and dextrin with the ratio of 1∶2；the wet mixing time was 30 min with 80% ethanol，and then they were passed through a 1.40 mm aperture sieve，and mixed for 30 min；the wet particles were dried at 70℃ for 4 h and coarse granules could be prepared with a 1.40 mm aperture sieve of a rocking granulator. Compared with traditional livestock and poultry Qingfei granules，the contents of epigoitrin and quercetin 3⁃O⁃β⁃D⁃glucose⁃7⁃O⁃β⁃D⁃gentiobioside were increased by 22.67% and 25.39% respectively，and the contents of liquiritin and ammonium glycyrrhizinate were doubled in modified livestock and poultry Qingfei granules.

Key words: Livestock and poultry Qingfei granule, Response surface method, Herb fermentation, Preparation process